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Access type: UnknownAuthor: search.filters.author.Chaiyawan, Neungnut

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    Characterization, comparative genomics, and metabolome analysis of Streptomyces tuberiscabiei sp. nov., a novel actinomycete causing potato scab disease in Long Khot, Chiang Mai Province, Thailand
    (2026-07-01)
    Inthong, Tanchanok
    ;
    Wannawong, Thippawan
    ;
    Mhuantong, Wuttichai
    ;
    Hongprasit, Aphinan
    ;
    Tanasupawat, Somboon
    Three actinomycete strains, WSLK1–3<sup>T</sup>, WSLK1–4, and WSLK1–5, associated with scabby potato tuber, were characterized using polyphasic and genome-based taxonomy. All strains were found to be Gram-stain-positive, filamentous bacteria, including LL-diaminopimelic acid in cell-wall peptidoglycan. Whole-cell sugars were glucose, mannose, rhamnose, and ribose. MK-9(H<inf>6</inf>) and MK-9(H<inf>10</inf>) were major menaquinones; C<inf>16:0</inf>, isoC<inf>16:0</inf>, ante iso-C<inf>15:0</inf>, and anteiso -C<inf>17:0</inf> were major cellular fatty acids; diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol mannoside were major phospholipids; and DNA G + C contents were 71.5 mol%. Phylogenetic analysis based on 16S rRNA gene and genome sequences indicated that strains WSLK1–3<sup>T</sup>, WSLK1–4, and WSLK1–5 are closely related to Streptomyces canus DSM 40017<sup>T</sup> (=JCM 4212<sup>T</sup>), and S. pseudovenezuelae DSM 40212<sup>T</sup> (=JCM 11516<sup>T</sup>), respectively. The 16S rRNA gene sequences, average nucleotide identity based on BLAST (ANIb) and MUMmer (ANIm), and digital DNA-DNA hybridization (dDDH) values among the three strains were 100%, 99.12%, 99.33%, and 94.3%, respectively, indicating that these strains belong to the same species. Strains WSLK1–3<sup>T</sup>, WSLK1–4, and WSLK1–5 showed 88.54–90.62% ANIb, 90.99–92.27% ANIm, and 40.4–45.1% to their closely related type strains: S. canus JCM 4212<sup>T</sup>, and S. pseudovenezuelae JCM 11516<sup>T</sup>. All novel strains were pathogenic, causing necrosis on potato tuber slices, inhibiting plant seedlings, and inducing superficial to raised scab lesions on potato tubers. Based on the phenotypic, chemotaxonomic, pathogenic, and genomic data, strains WSLK1–3<sup>T</sup>, WSLK1–4, and WSLK1–5 could be assigned to the novel species within the genus Streptomyces for which the name Streptomyces tuberiscabiei sp. nov. is proposed. The type strain is WSLK1–3<sup>T</sup> (=TBRC 19150<sup>T</sup> = LMG 33893<sup>T</sup>).
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    Evaluating the safety and efficacy of Lacticaseibacillus paracasei TISTR 2593 as a therapeutic probiotic for obesity prevention
    (2025-01-01)
    Sitdhipol, Jaruwan
    ;
    Niwasabutra, Kanidta
    ;
    Chaiyawan, Neungnut
    ;
    Nuankham, Kamonsri
    ;
    Thanagornyothin, Thanaphol
    Several recent studies have reported the potential of probiotics in reducing body weight and fat mass and improving glucose and lipid metabolism. Therefore, probiotic administration is considered an alternative approach for treating obesity. The objective of this study was to evaluate the probiotic properties and antiadipogenic potential of the strain TISTR 2593. Through whole-genome sequence analysis, the strain TISTR 2593 was identified as Lacticaseibacillus paracasei. L. paracasei TISTR 2593 exhibited γ-hemolytic activity (nonhemolysis) and demonstrated susceptibility to antibiotics, indicating that it is generally safe for consumption. Additionally, this strain displayed desirable probiotic properties, including tolerance to artificial gastric juice and bile salts, adhesion to Caco-2 cells, and the ability to inhibit pathogens. Furthermore, L. paracasei TISTR 2593 exhibited cholesterol-reducing capability and demonstrated antiadipogenic activity. In 3T3-L1 adipocytes, treatment with 10% (w/v) heated L. paracasei TISTR 2593 cells resulted in an approximately 50% reduction in lipid accumulation, similar to the positive control (quercetin). Moreover, L. paracasei TISTR 2593 heat-killed cells dose-dependently decreased the expression levels of CCAAT/enhancer-binding protein-α and peroxisome proliferator-activated receptor-γ, two vital transcription factors involved in the early stage of adipocyte differentiation. These findings suggest that L. paracasei TISTR 2593 possesses probiotic and functional properties, including antiadipogenic activity, supporting its potential as a therapeutic probiotic supplement for preventing obesity. Overall, the results of this study indicate that L. paracasei TISTR 2593 exhibits promising probiotic characteristics and beneficial effects on adipogenesis modulation, reinforcing its potential as a therapeutic option in obesity prevention.
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    Comparative genomics reveals insight into the phylogeny and habitat adaptation of novel Amycolatopsis species, an endophytic actinomycete associated with scab lesions on potato tubers
    (2024-01-01)
    Wannawong, Thippawan
    ;
    Mhuantong, Wuttichai
    ;
    Macharoen, Pipat
    ;
    Niemhom, Nantawan
    ;
    Sitdhipol, Jaruwan
    A novel endophytic actinomycete, strain MEP2-6<sup>T</sup>, was isolated from scab tissues of potato tubers collected from Mae Fag Mai Sub-district, San Sai District, Chiang Mai Province, Thailand. Strain MEP2-6<sup>T</sup> is a gram-positive filamentous bacteria characterized by meso-diaminopimelic acid in cell wall peptidoglycan and arabinose, galactose, glucose, and ribose in whole-cell hydrolysates. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and hydroxy-phosphatidylethanolamine were the major phospholipids, of which MK-9(H<inf>6</inf>) was the predominant menaquinone, whereas iso-C<inf>16:0</inf> and iso-C<inf>15:0</inf> were the major cellular fatty acids. The genome of the strain was 10,277,369 bp in size with a G + C content of 71.7%. The 16S rRNA gene phylogenetic and core phylogenomic analyses revealed that strain MEP2-6<sup>T</sup> was closely related to Amycolatopsis lexingtonensis NRRL B-24131<sup>T</sup> (99.4%), A. pretoriensis DSM 44654<sup>T</sup> (99.3%), and A. eburnea GLM-1<sup>T</sup> (98.9%). Notably, strain MEP2-6<sup>T</sup> displayed 91.7%, 91.8%, and 87% ANIb and 49%, 48.8%, and 35.4% dDDH to A. lexingtonensis DSM 44653<sup>T</sup> (=NRRL B-24131<sup>T</sup>), A. eburnea GLM-1<sup>T</sup>, and A. pretoriensis DSM 44654<sup>T</sup>, respectively. Based on phenotypic, chemotaxonomic, and genomic data, strain MEP2-6<sup>T</sup> could be officially assigned to a novel species within the genus Amycolatopsis, for which the name Amycolatopsis solani sp. nov. has been proposed. The type of strain is MEP2-6<sup>T</sup> (=JCM 36309<sup>T</sup> = TBRC 17632<sup>T</sup> = NBRC 116395<sup>T</sup>). Amycolatopsis solani MEP2-6<sup>T</sup> was strongly proven to be a non-phytopathogen of potato scab disease because stunting of seedlings and necrotic lesions on potato tuber slices were not observed, and there were no core biosynthetic genes associated with the BGCs of phytotoxin-inducing scab lesions. Furthermore, comparative genomics can provide a better understanding of the genetic mechanisms that enable A. solani MEP2-6<sup>T</sup> to adapt to the plant endosphere. Importantly, the strain smBGCs accommodated 33 smBGCs encoded for several bioactive compounds, which could be beneficially applied in the fields of agriculture and medicine. Consequently, strain MEP2-6<sup>T</sup> is a promising candidate as a novel biocontrol agent and antibiotic producer.