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Access type: UnknownAuthor: search.filters.author.Deewatthanawong, P.

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    Plant host selection for spore production of Glomas mosseae
    (2022-04-01)
    Deewatthanawong, R.
    ;
    Bannajit, W.
    ;
    Deewatthanawong, P.
    ;
    Bunya-Atichart, K.
    Five species including Ipomoea aquatica, Triticum aestivum, Oryza sativa, Zea mays, and Vigna radiata were used as plant hosts for spore production of Glomas mosseae. Seeds of all plant species were surface sterilized and grown in sterilized growing medium with a ratio of 1 part soil to 8 part sand. Each pot of each plant species was inoculated with 30 spores of G. mosseae and compared with the control without inoculum. Each treatment consisted of five replicates containing four pots. Half-strength Hoagland solution was applied once a week and plant growth parameters including diameter, height, and number of leaves were recorded every week for 2 months. Spores of G. mosseae in the rhizosphere, root colonization, and plant dry mass were measured after 2 months of inoculation. Results showed that G. mosseae improved plant growth of all plant species, with maximum increase in plant diameter found in Z. mays; while, maximum effects on plant height and root dry mass were observed in V. radiata. Results of spore formation suggested Z. mays as the most suitable host for mass spore production with the highest spore numbers of 97.4 100 g<sup>-1</sup> soil; however, no significant differences in root colonization were shown among the five plant species.
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    Influence of geographical and genetic variation on GABA content in Annona muricata grown in Thailand
    (2020-12-08)
    Deewatthanawong, R.
    ;
    Tontiworachai, B.
    ;
    Kongchinda, P.
    ;
    Chanapan, S.
    ;
    Sriwilaiwan, S.
    Annona is a genus of tropical fruit trees in the family Annonaceae. Fruit pulp of Annona muricata contained the highest amount of gamma-aminobutyric acid (GABA) among Annona species. GABA content results from many factors including genetic makeup and environment. Influences of genetic variation and geographical environment on GABA content in fruit pulp of A. muricata were determined. To study genetic variation, sequences of ITS2 and psbA-trnH were used to construct phylogenetic trees. Young leaves of A. muricata were collected from different geographical locations including Chumphon, Trang, Nakhon Ratchasima, Nakhon Si Thammarat, Buriram, Nong Khai, Udon Thani, and Prachuap Khiri Khan Provinces in Thailand. Sequences of ITS2 and psbA-tmH were aligned and data sets were analyzed using maximum likelihood (ML) and neighbor-joining (NJ) methods. ITS2 sequence variations were observed within and among samples from different locations. A. muricata fruit harvested from eight provinces, namely Chumpon, Trang, Nakhon Ratchasima, Nakhon Si Thammarat, Buriram, Nong Khai, Udon Thani, and Prachuap Khiri Khan contained 19.75, 35.02, 32.91, 28.18, 42.37, 32.17, 65.02, and 31.58 mg 100 g-1 FW GABA, respectively. Findings indicated that both geographical environment and genetic variation had significant effects on GABA content in A. muricata fruit.
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    GC-MS analysis and biopesticide properties of different crude extracts of Annona squamosa and Annona muricata
    (2019-01-01)
    Deewatthanawong, R.
    ;
    Kongchinda, P.
    ;
    Deewatthanawong, P.
    ;
    Pumnuan, J.
    ;
    Insung, A.
    Most common compounds identified by GC-MS analysis of ethanolic extracts from leaves of Annona squamosa (sweetsop) and Annona muricata (soursop) were neophytadiene, hexadecanoic acid, and trans-caryophyllene. Hexadecenoic acid and ethyl oleate were identified in seeds of A. muricata, while only methyl-p-tert-butyl phenylacetate was identified in seeds extract of A. squamosa. Crude extracts were tested for their antifungal activities against postharvest plant pathogens, insecticidal activities against brown planthopper, Nilaparvata lugens (Stål) and cassava mealybug, Phenacoccus manihoti Matile-Ferrero. Results showed low inhibitory effect (less than 20%) for the pathogenic fungi insecticidal test against cassava mealybug of all crude brown planthopper treated with 5% seed extract of A. squamosa, indicating its potential as an alternative botanical insecticide. Further studies are required to improve yield and extract quality.