KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/123456789/1

Browse

Search Results

Now showing 1 - 10 of 16

Some of the metrics are blocked by your
consent settings
Characterization and physicochemical properties of a novel microencapsulated bio-calcium from Asian sea bass bones
(2025-12-01)
Phengleng, Salinee
;
Sangadkit, Wipavadee
;
Wattanachaisaereekul, Songsak
;
Sirison, Jiraporn
;
Ruangsomboon, Suneerat
Calcium is the most abundant mineral in the human body, yet intake remains insufficient in many populations. Fishbone-derived bio-calcium from Asian sea bass (Lates calcarifer), containing approximately 37.5 % calcium (dry weight), offers a cost-effective source. However, its primary form, hydroxyapatite, has low solubility due to high crystallinity, limiting its application in food fortification. This study aimed to enhance the physicochemical properties of bio-calcium (B) powders by encapsulating them with maltodextrin (M), gum arabic (G), and their combination (MG) at 5 %, 10 %, and 15 % (w/v) using spray drying. A 1:4 (w/w) ratio of B to wall materials was applied at 180 °C (inlet) and 60 °C (outlet) temperatures. Powder yields ranged from 25.2 % (15 % BG) to 30.3 % (15 % BM), with no significant differences (p > 0.05) among treatments. Encapsulated powders had higher lightness (L*) than B. The highest calcium content and encapsulation efficiency were observed in 5 % BG, while BM showed the lowest. Moisture content and water activity remained below 10 % and 0.6 %, respectively. BG had the highest hygroscopicity, while wall concentration had no significant (p > 0.05) impact. Encapsulation improved water solubility index (75.4–86.5 %), especially in BM. Particle sizes ranged from 0.92 µm (10 % BMG) to 2.89 µm (15 % BM), while zeta potentials ranged from -8.71 mV (15 % BM) to -20.90 mV (15 % BMG). Encapsulated powders were more spherical and smoother than B, while BG particles showed aggregation, whereas BMG showed mixed morphologies. These findings suggest that encapsulation enhanced the physicochemical properties of bio-calcium, supporting its potential application in calcium-fortified foods and dietary supplements.
Some of the metrics are blocked by your
consent settings
Cultivation of Wolffia globosa and its application in functional food development
(2025-12-01)
Sirison, Jiraporn
;
Ruangsomboon, Suneerat
;
Jongput, Buppha
;
Aue-umneoy, Dusit
;
Tongsri, Pajongjit
This study assessed the biomass productivity, biochemical composition, and functional food potential of Wolffia globosa cultivated in four nutrient media: AB hydroponic solution, Chlorella medium, 16-16-16 fertilizer, and 16-16-16 supplemented with a vitamin B complex and FeSO<inf>4</inf> (16-16-16-B). Cultivation was conducted for eight weeks, with biomass harvested weekly. Among the treatments, the Chlorella medium produced the greatest biomass yield (133.82–236.00 g FW tank⁻1) and daily productivity (23.90–42.14 g FW m⁻2 day⁻1), significantly surpassing the other media (p < 0.05), followed by 16-16-16-B. Medium replenishment at four weeks initially enhanced growth but was later accompanied by a decline in biomass. Biochemical analysis indicated that the Chlorella medium yielded the highest protein content (46.10 ± 0.93% DW), while the AB medium supported the greatest chlorophyll-a (4.08 ± 0.00% DW) and carbohydrate levels (37.21 ± 1.12% DW); all differences among treatments were statistically significant (p < 0.05). Considering cost-effectiveness and nutritional value, the 16-16-16-B medium was optimal for cultivating W. globosa for food applications. Dried biomass from this medium was incorporated into fresh pasta and fried sweet potato balls, significantly enhancing chlorophyll-a, carotenoids, protein, fiber, and calcium contents. Sensory evaluation using a 9-point hedonic scale showed strong consumer acceptance even at higher inclusion levels. These findings highlight the role of nutrient-enriched media, particularly 16-16-16-B, in enhancing biomass yield and nutritional quality of W. globosa, affirming its potential as a functional food ingredient. Beyond nutritional enhancement, W. globosa-based foods exhibit immunomodulatory and anticancer potential through the antioxidant activity of pigments and other bioactive compounds.
Some of the metrics are blocked by your
consent settings
Enhancing biomass, hydrocarbon and biodiesel properties of green microalga Botryococcus braunii KMITL through gamma and UV radiation exposure
(2024-12-01)
Jongput, Buppha
;
Chiwpreecha, Pattanasak
;
Ruangsomboon, Suneerat
;
Tongsri, Pajongjit
This study investigated the effects of gamma (137Cs, 0–250 Gy) and UV (UV-C, 0–12 h) radiation on growth and biodiesel properties of Botryococcus braunii KMITL. For gamma radiation, maximum biomass (1.37 ± 0.02 g L−1) was achieved with 50 Gy, while a dose of 200 Gy resulted in the highest hydrocarbon content (51.84 ± 0.20%) and yield (0.66 ± 0.01 g L−1). For UV radiation, a 9 h exposure produced the highest biomass (2.45 ± 0.05 g L−1), hydrocarbon content (55.01 ± 1.22%), and yield (1.35 ± 0.04 g L−1). Algae exposed to gamma radiation within the range of 0–150 Gy exhibited C16:0 as the dominant fatty acid methyl ester (FAME), similar to those exposed to UV radiation, while algae exposed to 200–250 Gy displayed C18:1n9t as the dominant FAME. High levels of gamma and UV radiation were observed to lengthen fatty acid chains and increase unsaturated fatty acids. The cetane values of biodiesel from algae exposed to gamma and UV radiation ranged from 64.55 ± 0.14–66.47 ± 0.20 and 59.43 ± 0.04–65.27 ± 0.22, respectively, all meeting standard criteria. Both gamma and UV radiation also improved the saponification value and cold flow properties of the biodiesel. These findings suggest that controlled levels of gamma and UV radiation effectively enhance hydrocarbon yields with significant implications for biofuel production.
Some of the metrics are blocked by your
consent settings
Effect of CO2 Concentration on Growth, CO2 Fixation, and Biochemical Composition of the Microalga Nannochloropsis oculata
(2022-03-01)
Ruangsomboon, Suneerat
;
Chonudomkul, Duenrut
The green marine microalga Nannochloropsis oculata is rich in biochemicals and well known as a suitable source for dietary supplements and biodiesel feedstock. This study investigated the effects of CO<inf>2</inf> supplementation on N. oculata in terms of its biomass, biochemical composition, CO<inf>2</inf> fixation, and biodiesel qualities. Microalgae were cultivated in f/2 medium supplied with CO<inf>2</inf> at 0.04-6%. The culture supplemented with 6% CO<inf>2</inf> produced the highest biomass (1.64±0.04 g L−1) and contents of carotenoids (0.93±0.00 µg g−1), lipids (47.68± 2.80%), eicosapentaenoic acid (EPA; 28.45 mg g−1 biomass), and docosahexaenoic acid (DHA; 22.43 mg g−1 biomass); it also demonstrated the highest CO<inf>2</inf> fixation rate (74.62±2.55 kg m−3y−1) and provided an algal oil with desirable biodiesel qualities, specifically high cetane (52.27-53.50) and low iodine (75.91-82.15 g I<inf>2</inf> 100 g−1) values meeting the biodiesel specifications established by standards in the US (ASTM D6751) and Europe (EN 14214). In sum, CO<inf>2</inf> supplementation during N. oculata cultivation enhanced its production of high-value biomolecules, making this algal species a good source for production of dietary supplements and biodiesel.
Some of the metrics are blocked by your
consent settings
Outdoor open pond batch production of green microalga Botryococcus braunii for high hydrocarbon production: enhanced production with salinity
(2020-12-01)
Ruangsomboon, Suneerat
;
Dimak, Jantra
;
Jongput, Buppha
;
Wiwatanaratanabutr, Itsanun
;
Kanyawongha, Pornthiwa
The aim of this work was to enhance the biodiesel quality and hydrocarbon content of green microalga B. braunii strain KMITL 2 cultivated outdoor under several salinity conditions in a batch production. The enhancement would be such that the microalgal biodiesel qualities met or exceeded the current standard so that it would be a good raw material for biodiesel production. The microalga production was in 300 L open oval ponds, among various salinity levels tested (0–20 ppt), 5 ppt was the best for hydrocarbon production, yielding 54.2 ± 0.9% hydrocarbon content and 5.1 ± 0.4 g L−1 biomass. As the microalga production was scaled up by cultivation in 3,675 L open raceway pond under the 5 ppt condition, the microalga yielded a bit higher hydrocarbon content (58.8 ± 2.9%) but much lower biomass (2.5 ± 0.5 g L−1). The production in both oval and raceway ponds yielded a nearly identical biodiesel property (61.06–67.42 cetane number) which exceeded the value specified in international standards. Therefore, it was concluded that B. braunii strain KMITL 2 can be batch cultivated in an open pond at optimum salinity to yield sufficient hydrocarbon and biomass for biodiesel production.
Some of the metrics are blocked by your
consent settings
Hydrocarbon production and biodiesel properties of a green microalga Botryococcus braunii KMITL 2 cultivated outdoor in open pond and closed photobioreactor
(2018-03-01)
Ruangsomboon, Suneerat
Botryococcus braunii is a promising feedstock for biodiesel production. In general, selection of the most suitable cultivation systems is based on profitable yield and oil quality. The feasibility of using this alga as feedstock for commercial biodiesel production was tested by comparing the cultivation in open pond and closed photobioreactor under the 5 g⋅kg-1 optimum salinity condition previously found, and the results were that the hydrocarbon content (40.7±1.6%) of the alga cultivated in open pond was higher than that cultivated in the photobioreactor and its biomass (2.9±0.1 g⋅L-1) was much higher; its biodiesel properties, however, were nearly identical with cetane values of 48.15-56.99. Drastically different though were the hydrocarbon yields-cultivation in the open pond produced 2.3 times higher yield than the closed photobioreactor. Hence, outdoor cultivation under optimum salinity in open pond was considered suitable for biodiesel production by this algal strain.
Some of the metrics are blocked by your
consent settings
Enhanced hydrocarbon production and improved biodiesel qualities of Botryococcus braunii KMITL 5 by vitamins thiamine, biotin and cobalamin supplementation
(2018-01-01)
Ruangsomboon, Suneerat
;
Sornchai, Piyanuch
;
Prachom, Noratat
Botryococcus braunii, a green microalga which is a potentially good biodiesel feedstock, was isolated from a freshwater fish pond in Bangkok, and the effects of B-vitamins—thiamine, biotin and cobalamin as well as their mixes—on its biomass, hydrocarbon production, carbohydrate content and biodiesel properties were investigated, using a control group that was not supplemented with any vitamins. The addition of thiamine increased the alga's biomass and hydrocarbon content, whereas addition of biotin, cobalamin and mixes of all three vitamins did not increase its hydrocarbon content. Among the vitamins tested, thiamine 150 μg L− 1 was the best for producing high biomass (2.08 ± 0.07 g L− 1), hydrocarbon content (37.29 ± 0.41%), hydrocarbon yield (0.77 ± 0.03 g L− 1) and hydrocarbon productivity (90.32 ± 3.05 mg L− 1 d− 1). Maximum carbohydrate content (386 ± 39 mg g− 1) was achieved by addition of 1 μg L− 1 cobalamin. Compared to the control group, the alga cultured in media supplemented with 100 μg L− 1 thiamine showed better biodiesel properties with lower iodine value (76.42 g I<inf>2</inf> 100 g− 1), cold filter plugging point (9.90 °C) and higher cetane number (54.30). Therefore, thiamine was shown to be a promising culture medium supplement for this strain for using it as a feedstock for biodiesel production. An investigation of vitamin B-related gene in B. braunii KMITL 5 found related genes for thiamine (thi), biotin (bioB and bioF) and cobalamin (metE and metH) vitamins, which are marks of vitamin autotrophy in algae.
Some of the metrics are blocked by your
consent settings
Enhanced growth and hydrocarbon production of Botryococcus braunii KMITL 2 by optimum carbon dioxide concentration and concentration-dependent effects on its biochemical composition and biodiesel properties
(2017-01-01)
Ruangsomboon, Suneerat
;
Prachom, Noratat
;
Sornchai, Piyanast
The purposes of this study were to find the optimum level of supplementing CO<inf>2</inf> for increasing the biomass and hydrocarbon production of B. braunii KMITL 2 (A race) and to determine the effects of CO<inf>2</inf> level on CO<inf>2</inf> fixation and biodiesel properties. The experimental results showed that the alga supplemented with 10% CO<inf>2</inf> produced the highest biomass (1.48 ± 0.02 g L−1) and CO<inf>2</inf> fixation rate (100.43 ± 1.42 mg L−1 d−1) that were 2.7 and 5.3 times higher than the control (0.04% CO<inf>2</inf>). The gravimetric hydrocarbon content (36.82 ± 3.39%) and hydrocarbon titer (0.35 ± 0.03 g L−1) of the alga supplemented with 5% CO<inf>2</inf> were 1.6 and 2.7 times higher than the control. Cultivation of this strain under 5% CO<inf>2</inf> gave the highest hydrocarbon yield and good biodiesel properties with the lowest iodine value and a higher CN value than the ASTM D6751 and EN 14214 fuel standards.
Some of the metrics are blocked by your
consent settings
Effects of different media and nitrogen sources and levels on growth and lipid of green microalga Botryococcus braunii KMITL and its biodiesel properties based on fatty acid composition
(2015-06-08)
Ruangsomboon, Suneerat
This work aimed to find an optimum culture medium for green microalga Botryococcus braunii KMITL and investigate its biodiesel properties based on fatty acid composition. Four different media were tested. Chlorella medium was the best medium for lipid yield. Among four nitrogen sources tested, KNO<inf>3</inf> produced the highest lipid yield. When varied the nitrogen concentrations, this strain gave the highest lipid yield at the highest nitrogen level. When cultivated in the best medium and nitrogen source and level for 30 days, and then cultivated further for 14 days in the medium with no nitrogen, the highest lipid content and yield were 49.94 ± 0.82% and 2.71 ± 0.02 g L-1, respectively. C16:0 fatty acid was the major fatty acid found. Fatty acid profiles of B. braunii KMITL cultivated in Chlorella medium with 1.25 g L-1 KNO<inf>3</inf> gave the best biodiesel properties with the lowest iodine value, maximum cetane number, and lowest degree of unsaturation.
Some of the metrics are blocked by your
consent settings
Enhanced production of polysaccharides and protein content in cyanobacterium, Oscillatoria limnetica as a defense mechanism against low pH and Pb2+
(2015-01-01)
Ruangsomboon, Suneerat
Living biomass has better ability to remove Pb2+from wastewaters as compare to dead biomass. But, living biomass also have some practical limitations, such as, they are not efficient at low pH, and at high concentrations of metal ions. In the present study living biomass of Os cilia tori a Uni tie tic a was assessed for its metal sorption capacity. O. Bmnetica considerably sorbed a laige amount of Pb2+within pH range 3-7. The maximum Pb2+sorption capacity of 0. Unmetica was 434.78 mg/g. As photochemical efficiency data depicted, Pb2+exerted inhibitory effects on photosynthesis of the test cyanobacterium. However, the test cyanobacterium showed considerable tolerance to Pb2+exposure as concentration increased from 0 to 5 mg /L. Defense mechanisms of 0. Bmnetica under Pb2+enriched condition is probably associated with greater accumulation of intracellular polysaccharides (IPS) and protein. Nonetheless, since Pb2+binding capacity of 0. limnetica is significantly high than that of several other biosorbents, it can be considered as a promising biomaterial for removal of Pb2+from wastewaters.

KMITL

Browse

Filters

Settings

Sort By

Results per page

Search Results