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Potential enzymatic microbes for releasing plant nutrients and siderophore production
(2026-05-01)
Thongsri, B.
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Song, J. J.
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Soytong, K.
Burkholderia cepacia strain S001-08, Pseudomonas aeruginosa strain S001-11 and Pseudomonas fluorescens strain S001-15 were isolated from soil planted to Durian and selected for the properties to solubilize phosphate for plant nutrient and siderophore production for bioremediation. These selected strains were morphological and molecular phylogenic identification to confirm species level. B. cepacia strain S001-08, P. aeruginosa strain S001-11 and P. fluorescens strain S001-15 are found to be active responding as phosphorous solubilizers and producing siderophores. These active strains are being investigated to be synergistic effect and developing to be biological product as biostimulant and bioremediation. The developing biological product is promising to apply for crop production as an agricultural input for modern organic agriculture.
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Synergistic role of PGPR and fungal isolates in mitigating As and Pb stress and promoting growth of Arachis hypogaea L.
(2026-01-01)
Sathya, C.
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Sridhar, D.
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Soytong, K.
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Song, J. J.
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Lalitha, S.
Heavy metals, also known as toxic metals, are major environmental pollutants that adversely impact all forms of life, disturbing soil ecology and reducing agricultural productivity. Certain indigenous microbes possess remarkable tolerance to these metals and play a vital role in restoring contaminated soils. In the present study, plant growth-promoting rhizobacteria and fungi were isolated from the rhizosphere and evaluated for their metal tolerance potential. Among the bacterial isolates (Pseudomonas alcaliphila PAS1, Pseudomonas aeruginosa PAS2, Pseudomonas toyotomiensis PTS3, Bacillus subtilis BSS1) expressing resistance to arsenic and lead which observed up to concentrations of 100–1200 ppm on nutrient agar. The fungal isolate Aspergillus japonicus (AJ01) exhibited tolerance up to 200 ppm of Pb and 500 ppm of As. Pot culture experiments were using Arachis hypogaea grown under As and Pb stresses with treatments of PGPR, PGPF, Compost and chemical control. Pseudomonas alcaliphila PAS1 was significantly enhanced plant growth by 100% and Pseudomonas aeruginosa PAS2 by 60% in contaminated soil, indicating strong tolerance and growth-promoting potential. Pseudomonas alcaliphila PAS1 increased chlorophyll content by 92.3% under As stress as compared to the control, while Pseudomonas toyotomiensis PTS3 showed a 70% increased, demonstrating high metal tolerance. In contrast, As and Pb stressed plants showed adverse effects. These findings highlighted the potential of selected PGPR and fungi as eco-friendly alternatives for the remediation and recovery of heavy metal-contaminated soils in Arachis hypogaea cultivation.
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Antagonistic effects of Chaetomium globosum and Trichoderma viride against Chili Anthracnose
(2026-01-01)
Rohaendi, P.
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Sudjatmiko, S.
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Pamekas, T.
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Soytong, K.
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Song, J. J.
The research demonstrated that Trichoderma viride and Chaetomium globosum inhibited Colletotrichum acutatum by 90.30% and 88.20%, respectively, primarily via competition for nutrients/space and parasitism, with no observed antibiosis. the field trials revealed that both fungi significantly reduced anthracnose severity. Trichoderma viride applications at 1, 2, and 3 g/L decreased disease incidence by 80.68%, 91.16%, and 92.27%, respectively, while Chaetomium globosum reduced it by 87.61–92.65%. Both exceeded synthetic fungicide Antracol (83.48–89.37% reduction) as a controlled treatment. Attack intensity followed a similar trend, with Trichoderma viride (95.12–97.46% suppression) and Chaetomium globosum (92.76–96.83%) surpassing Antracol (89.95–93.80%). Additionally, Trichoderma viride and Chaetomium globosum enhanced salicylic acid levels in chili plants, suggesting induced systemic resistance. These findings highlighted the potential of antagonistic fungi as sustainable biocontrol agents which would develop to be biofungicide, offering effective anthracnose management while reducing chemical residues.
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AI-driven precision agriculture in Eurasia-coordinated innovation for belt and road agricultural transformationin
(2025-11-01)
Mitrokhin, M.
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Soytong, K.
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Song, J. J.
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Mitrokhin, E.
International collaboration in AI based technologies in agriculture is essential factor to increase output and agriculture productivity. AI horizons/ alliance program aimed to increase awareness and accessibility of new agro markets and optimize supply routes in Eurasia. Precision agriculture is commonly used term that enables AI based instruments into practical agriculture works. Precision agriculture in Eurasia is undergoing a transformative wave powered by artificial intelligence (AI) and cross-border innovation. This article examines how AI-driven technologies including agro-drones for aerial crop monitoring, satellite-based NDVI analysis, image recognition for pest and disease detection, advanced weather forecasting models, and decision support systems – are being deployed across Great Eurasia and Belt and Road Initiative (BRI) countries to boost agricultural productivity and sustainability. A particular focus is given to the Eurasian context countries like China, Kazakhstan, Uzbekistan, Russia and Thailand are leveraging AI to overcome climate and labor challenges, increase yields, and optimize resource use. The AI Horizons Alliance, a Sber-driven consortium, is highlighted as a coordinating body facilitating the exchange of AI technologies and expertise across borders. Real-world case studies demonstrate tangible benefits, such as up to 20% increases in crop yields, and 30% improvements in efficiency, and significant cost savings, achieved through AI-enabled precision farming. The article discusses the collaborative efforts under BRI frameworks, the integration of traditional knowledge with cutting-edge digital tools, and the role of international organizations in guiding this tech-driven agricultural transformation. Challenges from data sharing to capacity building are considered alongside prospects for broader adoption. The findings underscore that AI-driven precision agriculture, underpinned by coordinated Eurasian partnerships, holds to promise for enhancing food security and sustainable development across the region. It is a part of innovation organic agriculture.
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Biosurfactant from Streptomyces zaomyceticus HUS20 isolated from the Indian Himalayan Region (IHR) and its antibacterial activity
(2025-07-01)
Kishorekumar, A.
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Radhakrishnan, M.
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Manigundan, K.
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Ranjani, S.
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Thenmozhli, G. S.
Actinobacteria, which are known to synthesize bioactive molecules with significant industrial and medicinal applications. In order to assess the possibility for producing biosurfactant (BS), this study identified culturable actinobacteria from the high-altitude soils of Drama Valley, Dugtu Village in the Indian Himalayan Region (IHR). Actinobacteria were isolated from pre-treated soil samples using nutrient agar, starch casein nitrate agar, and actinomycetes isolation agar, which were enhanced with 1% crude oil. Using the haemolytic assay, oil displacement test, emulsification activity, drop collapse assay, and penetration assay, morphologically different colonies were proved for the production of BS. In this study, a promising biosurfactant-producing strain, strain HUS20 showed considerable emulsification index (65%) and a 30 mm zone in the oil displacement test. Using 16S rRNA sequencing, the strain HUS20 was shown to be 100% identical to Streptomyces zaomyceticus (GenBank accession: OQ996835.1). in HR LC MS analysis, the crude biosurfactant extract showed the presence of more than 164 metabolites. Additionally, the crude biosurfactant demonstrated strong antibacterial action against foodborne pathogens, with Bacillus cereus showing the greatest suppression (24 mm zone at 100 mg/ml). The potential of actinobacteria from harsh Himalayan habitats as a useful resource for the synthesis of biosurfactants is highlighted by this work. Streptomyces zaomyceticus HUS20 is found to be promising strain for isolation of potential biosurfactant molecule.
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Bio-theraprutic intervention for dengue infection-present scernario on plant-based remedies: A review article
(2024-03-01)
Lavanya, D.
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Parthasarathy, K.
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Soytong, K.
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Sounderrajan, V.
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Manickam, R.
Dengue virus (DENV), a member of the Flaviviridae, is the causative organism of Dengue hemorrhagic fever and Dengue Shock Syndrome. It exists as 4 serotypes (DEN1-4), all of which can cause full spectrum of the disease. Dengue is one of the most re-emerging arboviral diseases in the world with a record of 390 million cases globally, putting 3.9 billion people at the risk of infection annually. With dengue being enlisted as a high burden disease in the tropical countries, there is a dire need to bring about efficient therapeutics that help to prevent and treat the diseased population. The present mode of treatment of Dengue is based on the management of symptoms and includes the use of Non-steroidal Anti-inflammatory Drugs (NSAIDs), antibiotics, antipyretics and corticosteroids. These provide symptomatic treatment and also bring about undesirable side effects. Presently, there are no approved vaccines or antivirals for Dengue treatment in India. It is thus important to consider the biology of the virus with special focus on identification of effective anti-viral targets for therapy. With the alleviation of symptoms alone being insufficient for the disease treatment, there is a shift in research to find alternative forms of medicine for the treatment of the disease. Herbal remedies have a long history of use in ancient medical systems and are now widely accepted as safe, natural substitutes for pharmaceutical treatments. But in order to guarantee consistency and safety, the growing demand for these items calls for strict quality control methods. In particular, leaves of Carica papaya and Nilavembu kudineer are potential candidates for this purpose. This review focuses on bringing to light the various therapeutic interventions presently in use and emphasize the significance of quality control techniques in regulating various aspects of the development, manufacturing, and distribution of herbal products.
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Organic agriculture practises and approaches to certification of Organic agriproducts: Review article
(2024-01-01)
Chumak, K.
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Soytong, K.
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Song, J. J.
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Mitrokhin, M.
Organic certification has encouraged for reliable the consumer demand to get safety food. Association of Agricultural Technology in Southeast Asia (AATSEA) has started irganically certified in the year of 2015 by following the organic standard from many certify bodies eg., International Federation of Organic Agriculture Movements (IFOAM) by BioAgriCert, organic USDA etc. The main target is to examine organic production without application of agrochemicals (chemical fertlizers, chemical pesticides and other synthrtic chemicals) through environmental protection surrounding of soil, water, air and biodiversity for sustainability. In 2022 AATSEA has proposed an dynamic evolution of modern organic agriculture for certification. AATSEA Organic standard is approved by the committee which concerns to the final organic products will not detect toxic agrochemicals, formalin, nitrate/nitrite, non-GMO, low standard of heavy metals as well as Escherichia coli, Samonella sp. The burgeoning logistics and supply chains for agricultural products across Eurasia necessitate robust interaction and expert dialogue among relevant certification bodies. This is crucial for fostering harmonisation and mutual recognition of organic products between RusQuality, Russia and AATSEA Organic certification.
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Fish gut microbiota: a source of novel metabolites – A review article
(2023-03-01)
Jisha, K.
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Gayathri, G.
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Gopikrishnan, V.
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Song, J. J.
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Soytong, K.
Similar to human beings, fish harbor microorganisms in their gastrointestinal tract (GIT). Fishes are diverse groups of gut microbiota, including protists, fungi, yeasts, viruses, bacteria, and archaea. In addition to serving as a barrier against infections, these microbes that inhabit the gastrointestinal tract of fish play a role in nutrition, physiology, immunity, and life span. All fish have gut microbiota; however, the makeup of these communities varies depending on the fish's life stages, the habitat they live in, their nutrition, the seasons, their trophic level, etc. For both recreational and commercial fisheries, it is crucial to comprehend the bacterial make-up of fish microbiomes. The gut microbiota aids in the development of methods for modifying the gut microbiota of the target fish species to enhance aquaculture quality. These gut microbes are an invaluable, essential source of novel, promising bioactive compounds with significant biological activity. The natural product secondary metabolites from specific strains of Chaetomium spp. may develop to be biomedicine for sustainable protection. This review provides a comprehensive knowledge of the composition of the gut microbiota of fish, their development, changes in the living environment, their modification, and their applications.
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Exploration of earthwormcast associated actinobacteria for plant growth promoting properties
(2023-01-01)
Ashok Kumar, S.
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Karthiga, P.
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Rajalakshmi, G.
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Gopikrishnan, V.
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Radhakrishnan, M.
Due to their wide varieties of chemicals with a variety of biological actions, earthworms, cast, and gut are potential sources of significant biotechnological interest. The finding new microorganisms in peculiar and uncharted habitats were investigated. Actinobacteria are exceptional makers of secondary metabolites and have a wide range of bioactive substances of economic significance. In the current investigation, actinobacteria from earthworm casting were isolated for their potential antibacterial capabilities. Earthworm cast from the Kanchipuram agricultural area had a total of 12 actinobacterial colonies. Human and plant pathogens such as Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa and Ralstonia solanacearum were tested against each actinobacterial cultures. The selected potential culture was examined for the presence of Indole Acetic Acid (IAA), Phosphate (P) solubilization, extracellular enzymes such as cellulase and amylase. For the promising active trains, optimization, characterization and taxonomy were carried out. Colonies with various morphologies were separated and subcultured for testing their activities. Six of the twelve isolates proved positive for antibacterial activity against one or more pathogens. Of the six isolates tested, strain EWCA 21 was chosen for further screening because it had the greatest effectiveness against tested pathogens. IAA, Phosphate (P) solubilization, and extracellular enzyme activities such amylase and cellulase were demonstrated in EWCA21. Carbon and nitrogen sources expressed an increase in activity and output during optimization. The putative strain was determined to be Streptomyces based on phenotypic analysis. The study concluded that the strain EWCA21 would be developed as a crucial resource for biofertilizer applications.
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New discovery of natural product nanoparticles constructed from active metabolites from Chaetomium brasiliense for immunity to brown spot of rice var. RD 47
(2023-01-01)
Vareeket, R.
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Song, J. J.
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Soytong, K.
Natural product nanoparticles constructed from active metabolites from Chaetomium brasiliense is firstly presented as a new elicitor for immunity to brown spot of rice var. RD 47 caused by Drechslera oryzae. The nano-CBH, nano-CBE, nano-CBM from C. brasiliense at 15 μg/ml are treated to rice leaves after 3 days expressed R<inf>f</inf> of 0.61 which supposing to be Oryzalexin C. Moreover, ethyl acetate, hexane and methanol crude extract derived from C. brasiliense that shown to be inhibited D. oryzae which the ED<inf>50</inf> were 0.24, 0.32 and 0.35 μg/ml, respectively. Nano-CBH, nano-CBE, nano-CBM inhibited D. oryzae with ED<inf>50</inf> values of 5.86, 4.92, and 2.86 μg/ml, respectively. All nanoparticles derived from C. brasiliense were more actively responded to suppress the pathogen inoculum of D. oryzae at the lower concentration than crude extracts at lower concentrations.

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