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Access type: UnknownAuthor: search.filters.author.Yamada, Yuzo

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    Statistical optimisation of culture conditions for biocellulose production by Komagataeibacter sp. PAP1 using soya bean whey
    (2014-02-18)
    Suwanposri, Amornrat
    ;
    Yukphan, Pattaraporn
    ;
    Yamada, Yuzo
    ;
    Ochaikul, Duangjai
    A low-cost medium based on soya bean whey (SBW) was prepared as a by-product from agriculture and optimised for biocellulose (BC) production by Komagataeibacter sp. PAP1. The optimal conditions for BC production are: pH 6.21, 1.61% ethanol concentration (v/v) and 28.4°C. The use of optimised medium based on SBW increased BC production 3.6 fold compared to standard Hestrin-Schramm (HS) medium. The BC film produced from the optimised medium was stronger and more impermeable to water vapour and oxygen compared to the one produced from the standard HS medium. These properties allow SBW film to be developed into eco-friendly food packaging for oxygen-sensitive products. Its high water absorption capacity allows its use in biomedical application such as wound dressing. Our results demonstrate that SBW can be used as an alternative low-cost substrate for BC production on commercial scale. © 2014 by Maejo University, San Sai, Chiang Mai, 50290 Thailand.
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    Identification and biocellulose production of Gluconacetobacter strains isolated from tropical fruits in thailand
    (2013-03-28)
    Suwanposri, Amornrat
    ;
    Yukphan, Pattaraporn
    ;
    Yamada, Yuzo
    ;
    Ochaikul, Daungjai
    Two hundred and four strains of biocellulose (BC)-producing Gluconacetobacter strains were isolated from 48 rotten tropical fruits collected in Thailand. Twenty-nine representative isolates were selected from each of the 16 isolation sources and identified by morphological, physiological and biochemical characteristics and 16S rRNA gene sequence analysis. The selected 29 isolates were divided into seven subgroups within the Gluconacetobacter xylinus group of the genus Gluconacetobacter and identified as Gluconacetobacter oboediens (subgroup I, five isolates), Gluconacetobacter rhaeticus (subgroup II, one isolate), Gluconacetobacter hansenii (subgroup III, seven isolates), Gluconacetobacter swingsii (subgroup IV, two isolates) and Gluconacetobacter sucrofermentans (subgroup V, two isolates). The remaining isolates were grouped into subgroups VIa (three isolates) and VIb (nine isolates). All the isolates were cultured in Hestrin-Schramm (HS) medium statically at 30°C for 7 days to determine cellulose production capability. Of the 29 isolates, isolate PAP1 (subgroup VIb, unidentified) gave the highest yield (1.15 g/L) of BC. However, the BC yield increased threefold (3.5 g/L) when D-glucose in HS medium was replaced by D-mannitol. © 2013 by Maejo University, San Sai, Chiang Mai, 50290 Thailand. Reproduction is permitted for noncommercial purposes.
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    Description of Komagataeibacter gen. nov., with proposals of new combinations (Acetobacteraceae)
    (2012-11-23)
    Yamada, Yuzo
    ;
    Yukphan, Pattaraporn
    ;
    Vu, Huong Thi Lan
    ;
    Muramatsu, Yuki
    ;
    Ochaikul, Duangjai
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    Subdivision of the genus Gluconacetobacter Yamada, Hoshino and Ishikawa 1998: The proposal of Komagatabacter gen. nov., for strains accommodated to the Gluconacetobacter xylinus group in the α-Proteobacteria
    (2012-06-01)
    Yamada, Yuzo
    ;
    Yukphan, Pattaraporn
    ;
    Vu, Huong Thi Lan
    ;
    Muramatsu, Yuki
    ;
    Ochaikul, Duangjai
    The genus Gluconacetobacter is divided into two groups phylogenetically, phenotypically and ecologically: the Gluconacetobacter liquefaciens group and the Gluconacetobacter xylinus group. For the latter group, the genus Komagatabacter is newly introduced, and the type species of the new genus is designated as Komagatabacter xylinus (Brown 1886) comb. nov. Twelve species of the Gluconacetobacter xylinus group are transferred to the new genus as new combinations. © Springer-Verlag and the University of Milan 2011.