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Item type:Publication, Valorization of banana peel for citric acid production under solid state fermentation with aspergillus Niger(2020-01-01)Chysirichote, T.Valorization of banana peel (BP) through solid state fermentation (SSF) was aimed in this research. The appropriate conditions of citric acid (CA) production by SSF of Aspergillus Niger were investigated. Firstly, the optimum initial pH of the BP and the aeration rate were studied by conducting SSF in a 250-mL flask and 2-L glass column, respectively. It was found that the initial pH of the BP and aeration rates affected the CA production. The results showed that the initial pH of 5.0 and 1.0 vvm aeration were appropriate for the CA production of A. Niger using BP as a substrate. The problem of rising temperature during SSF was found when applying the optimum condition to the SSF in the 20-L packed bed bioreactor (PBB), which caused a decrease in the CA production compared to that of the glass column. The cooling air jacket constructed to the PBB to remove the heat during the SSF helped increase the CA production from that in the PBB. The maximum CA production in the 20-L air-jacketed PBB was 124.0±19.2 mg g<sup>-1</sup><inf>DS</inf>. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Cellulase production by Aspergillus Niger ATCC 16888 on copra waste from coconut milk process in layered packed-bed bioreactor(2018-07-01)Chysirichote, T.Solid-state fermentation (SSF) has been used to produce value-added products from agricultural and food processing wastes, but the majority of SSF production has been conducted on a small scale due to the limitations of heat and mass transfer. This research aimed to reduce the temperature changes in packed-bed bioreactor during the cellulase fermentation of Aspergillus Niger ATCC 16888 on copra waste from coconut milk process. The three configurations of bioreactor used in this study consisted of one-(control), two- and three-layered packed-bed bioreactors. Total volumes of packed-bed bioreactor and bed material were 127 and 57 L, respectively. The sterilized air 30 °C was forced from the bottom of bioreactor at 0.20 vvm. The highest cellulase production (5.0 ± 0.4 FPU g<sup>-1</sup> <inf>DS</inf>) was obtained from the bottom zone of the three-layered bioreactor after fermenting for 1 d. However, the greatest growth was found at the top one, in which a large amount of aerial mycelium was detected.