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Author: search.filters.author.Arananant, J.

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    In vitro propagation from nodal segments of Arachis glabrata cultivar Florigraze
    (2020-09-01)
    Pholjad, A.
    ;
    Pongtongkam, P.
    ;
    Arananant, J.
    ;
    Poeaim, A.
    Arachis glabrata Benth. of rhizome perennial peanut is used as a forage plants with higher nutrient value. The nodal segments were used to propagate in vitro by 0.2% of HgCl<inf>2</inf>plus antibiotic and cefotaxime and preservative for plant tissue culture media active (PPM) at 15 min. It was the most effective procedure for disinfecting plant parts (survival of 73.33%). Shoot induction used to the nodal segments of A. glabrata cultivar Florigraze were cultured on MS medium combination with plant growth regulators in cytokinin group at different concentrations 0.5, 0.75, 1, 2, 3 and 5 mg L<sup>-1</sup>of 6-benzylaminopurine (BAP), N6- furfuryladenine (Kn), meta-Topolin (mT) and Thidiazuron (TDZ). The maximum growth rate was 66.67% on 5 mg L<sup>-1</sup>of mT was the highest shoot length of 18.43 mm after 30 days. Root induction was achieved on MS medium supplemented with 2 mg L<sup>-1</sup>of α-Naphthaleneacetic acid (NAA) and 0.2% Activated Charcoal (AC) after 8-12 days cultured when compared with MS medium without plant growth regulators at the basal condition. Thereafter, the plantlets were transferred into the soil for acclimatization. It is the first report using the nodal segment in vitro propagation.
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    In vitro effect of gamma irradiation and plant growth regulators (PGRs) for induction and development of Stylosanthes hamata cv. Verano
    (2019-01-01)
    Ngoenngam, L.
    ;
    Pongtongkam, P.
    ;
    Arananant, J.
    ;
    Poeaim, S.
    ;
    Poeam, A.
    Pasture legumes was developed by tissue culture technique using gamma irradiation. Seeds of Stylosanthes hamata cv. Verano were received 7 doses of radiation (5, 10, 15, 20, 25, 30 and 45 Krad) and control (without irradiation) cultured on MS medium. Free Plant Growth Regulators (PGRs) and 30 g/L sucrose treatment found that 30 Krad decreased the percentage of shoot and root length of 31.7% and 20.51% respectively in 30 days. While seeds without irradiation were 100% germination. The radiation doses was reduced the growth rate by half in 7 days GR <inf>50</inf> (3 <inf>0)</inf> at 39.82 Krad. The lethal dose (LD <inf>50</inf> ) of Stylosanthes hamata cv. Verano was 26.14 Krad and the higher irradiation level increased death rate. Seeds on MS medium with 3 mg/L of meta-Topolin (mT) resulted at 60 days after irradiation which higher than 50 % in all treatments. Moreover, every dose of gamma rays stimulated shoots and roots after 60 days.
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    In vitro effect of plant growth regulators (PGRs) for callus induction and plant regeneration from suspension of Hamata (Stylosanthes hamata cv. Verano)
    (2018-12-01)
    Ngoenngam, L.
    ;
    Pongtongkam, P.
    ;
    Arananant, J.
    ;
    Poeaim, S.
    ;
    Poeaim, A.
    The plant regeneration from seed of Hamata (Stylosanthes hamata cv. Verano) was found. Seeds were induced on MS medium (Murashige and Skoog, 1962) with different concentration of cytokinin were used 0.5, 1 and 3 mg/L 6-benzyladenine (BAP) meta-Topolin (mT) or Thidizuron (TDZ), respectively. The result showed that the highest percentage of seed induction was 65% and average shoots 8.66 shoots per seed on MS medium with 3 mg/L mT for 12 weeks cultivation. For node of hamata were study on callus and shoot induction on MS medium with 0.5 mg/L IBA combined with 0.5, 1 and 3 mg/L TDZ. The highest percentage of callus and shoot induction was 100% gave average areas of callus at 11192.84 mm <sup>3</sup> and the number of shoots per callus was 13.50 shoots on MS medium with 0.5 mg/L IBA combined 3 mg/L TDZ for 8 weeks cultivation Cell suspension culture of callus that developed from node of hamata. The friable callus was transferred to liquid MS medium with 0.5 mg/L IBA combined with 3 mg/L TDZ for 4 weeks cultivation After that transferred to MS medium with 3 mg/L mT for shoots regeneration. The percentage of shoots generation from callus was shown on 90% and the number of shoots per callus was 14.00 shoots for 12 weeks cultivation. Transferred shoot to root medium containing of MS medium with Indole-3-butyric acid (IBA) Naphthaleneacetic acid (NAA) or Indole-3-acetic acid (IAA) at 0.1, 0.3 and 0.5 mg/L. The result showed that the highest percentage of root induction was 100% and gave averaged roots at 11.00 <sup>+</sup> 1.87 roots per shoot on MS medium with 0.3 mg/L NAA for 12 weeks cultivation.