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Author: search.filters.author.Borompichaichartkul, ChaleedaSubject: search.filters.subject.Anti-glycation activity

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    Item type:Publication,
    Enzymatic hydrolysis and biological activities of Konjac glucomannan hydrolysate in different degree of polymerisation
    (2024-11-01)
    Pomsang, Pachara
    ;
    Ayuni, Dwi
    ;
    Phumsombat, Putthapong
    ;
    Eugelio, Fabiola
    ;
    Fanti, Federico
    The study explores the enhanced functional and bioactive properties of Konjac Glucomannan Hydrolysate (KGMH) by partially degrading Konjac Glucomannan (KGM) using β-mannanase over 60 min. KGM concentrations (40% and 50% w/w) were treated with 200–300 U g<sup>−1</sup> of the enzyme over 60 min. The hydrolysis of KGM was monitored by colorimetry, with DP values ranging from 4.20 to 6.16 for 40% KGM and 4.10 to 4.60 for 50% KGM. MALDI-TOF-MS analysis confirmed typical oligosaccharides with DP values from 2 to 9 and some acetyl substitutions. The optimal conditions of 40% KGM with 250 U g<sup>−1</sup> enzyme and varying hydrolysis times produced KGMHs with a wide range of DPs, demonstrating in vitro antioxidant and anti-glycation activities. The results showed significant bioactivities (P < 0.05) positively correlated with lower DP values. This study emphasises the potential of KGMH as a novel functional food ingredient, highlighting its bioactive properties and the significant impact of DPs on the biological functionality of saccharides.