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Author: search.filters.author.Bunbamrung, NantiyaSubject: search.filters.subject.Streptomyces

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    Streptomyces tyrfis sp. nov., an actinobacterium isolated from Thai peat swamp forest soil and its promising secondary metabolites
    (2026-12-01)
    Kottip, Penkawee
    ;
    Klanbut, Khanungkan
    ;
    Fukasem, Poowadon
    ;
    Pittayakhajonwut, Pattama
    ;
    Intaraudom, Chakapong
    An actinomycete strain, PTD5-9<sup>T</sup>, was isolated from peat swamp forest soil in Narathiwat Province, Thailand. Chemotaxonomic features and phylogenetic analysis based on the 16S rRNA gene sequence placed this strain within the genus Streptomyces. It exhibited the highest sequence similarity to Streptomyces gelaticus NRRL B-2928ᵀ (99.38%) and Streptomyces sanglieri NBRC 100784ᵀ (99.31%). However, the average nucleotide identity based on MUMmer (ANIm) values (88.48–88.56%), the average amino acid identity (84.37–84.97%), and digital DNA–DNA hybridization (dDDH) estimates (31.2–36.5%) were significantly below the accepted thresholds for species demarcation, supporting its classification as a new species within the genus. Accordingly, the name Streptomyces tyrfis sp. nov. is proposed. The crude extract derived from the culture broth of strain PTD5-9ᵀ exhibited potent antibacterial activity against Gram-positive bacteria (MIC ≤ 250 µg/mL). Subsequent chemical analysis led to the identification of ten bioactive secondary metabolites: isocycloheximide (1), (2R,4S,6E)-anhydrocycloheximide (2), actiphenol (3), (−)-phenatic acid A (4), 8-O-methyltetrangomycin (5), 8-O-methylrabelomycin (6), 4-hydroxy-8-O-methylrabelomycin (7), tetrangulol methyl ether (also known as X-14881 E) (8), (−)-elmonin (9), and 2-phenylacetamide (10). Among these, compounds 3, 6, 7, and 8, which were isolated in sufficient quantities, exhibited notable inhibitory activity against Mycobacterium tuberculosis, with MIC values ranging from 3.13 to 25.0 µg/mL. Furthermore, compounds 6, 7, and 8 demonstrated pronounced cytotoxicity against human small cell lung cancer (NCI-H187) cells (IC₅₀: 1.10–7.80 µM), and against human breast cancer (MCF-7) cells (IC₅₀: 1.21–24.6 µM).
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    Streptomyces telluris sp. nov., a promising terrestrial actinobacterium with antioxidative potentials
    (2023-06-01)
    Thayanuwadtanawong, Onnicha
    ;
    Duangupama, Thitikorn
    ;
    Bunbamrung, Nantiya
    ;
    Pittayakhajonwut, Pattama
    ;
    Intaraudom, Chakapong
    An actinomycete strain, AA8<sup>T</sup>, which produced a long straight chain of spores (verticillati type), was isolated from the rhizosphere soil of Mangifera indica in Bangkok, Thailand. A polyphasic taxonomic study was carried out to establish the taxonomic position of the strain. Strain AA8<sup>T</sup> formed a tight taxonomic position in the 16S rRNA gene tree with Streptomyces roseifaciens MBT76<sup>T</sup>. In contrast, the genome-based taxonomic analysis showed that strain AA8<sup>T</sup> shared low average nucleotide identity-BLAST (94.1%), the digital DNA–DNA hybridization (58.2%), and the average amino acid identity (93.6%) values with S. roseifaciens MBT76<sup>T</sup>. Moreover, a combination of physiological and biochemical properties indicated that strain AA8<sup>T</sup> was distinguished from all Streptomyces species with effectively published names. Strain AA8<sup>T</sup>, therefore, represents a novel species of Streptomyces, and the name Streptomyces telluris is proposed for the strain. The type strain is AA8<sup>T</sup> (= TBRC 8483<sup>T</sup> = NBRC 113461<sup>T</sup>). The chemical investigation led to the isolation of nine known compounds (compounds 1–9). Among these compounds, compound 7 (3,4-dihydroxybenzaldehyde) possesses strong antioxidant activity equal to ascorbic acid, a powerful antioxidative agent.
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    Antibacterial, antitubercular, antimalarial and cytotoxic substances from the endophytic Streptomyces sp. TBRC7642
    (2020-04-01)
    Bunbamrung, Nantiya
    ;
    Intaraudom, Chakapong
    ;
    Dramae, Aibrohim
    ;
    Thawai, Chitti
    ;
    Tadtong, Sarin
    Eight previously undescribed naturally-occurring compounds, including abyssomycins Y - Z, methyl aeruginoate, desferri-ferrithocin-4-hydroxyphenethylester, streptomethiocins A - B, furaquinocin I, and streptolactone, along with eleven known compounds were isolated from the endophytic Streptomyces sp. TBRC7642. The chemical structures were determined based on spectroscopic means including 1D, 2D NMR spectroscopy and mass spectrometry. The absolute configurations were assigned by relying on CD spectra and their optical rotations. In addition, the isolated compounds were evaluated for biological activity, such as antimalarial, antitubercular, antibacterial (both Gram-positive and Gram-negative bacteria), as well as for cytotoxicity against MCF-7, NCI–H187, and Vero cells.