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Application of Melaleuca cajuputi Powell crude extract on controlling postharvest anthracnose disease in Gross Michel banana (Musa (AAA group) ‘Kluai Hom Thong’)
(2026-03-01)
Montri, N.
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Leujantuak, P.
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Tongsri, V.
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Deewatthanawong, R.
;
Bunya-Atichart, K.
Exporting Gross Michel bananas, known as ‘Kluai Hom Thong’ in Thailand, to Japan involves several considerations to ensure compliance with Japan’s stringent food safety and quality standards, especially regarding pesticide residues and other contaminants. The research aimed to evaluate the impact of various concentrations of Melaleuca cajuputi Powell crude extract on controlling postharvest anthracnose disease in Gross Michel bananas, focusing on developing a non-toxic production method. The 70% ripening stage bananas were dipped with 0, 800, 1,000, and 1,200 mg L-1 crude extract for 2 min before spraying with 10-6 spore mL-1 of Colletotrichum musae spore suspension, followed by a 2-day incubation, subsequent spraying with 500 mg L-1 of ethephon solution, and a further 1-day incubation at ambient temperature (29±2°C). The shelf-life of banana, weight loss, total soluble solids, and surface morphology of banana peel were analyzed. Results showed that applying M. cajuputi Powell crude extract could extend shelf-life and maintain the quality of banana fruits. The 800 mg L-1 crude extract resulted in 2.13% weight loss, with peel density at 14.80 N cm-3 and pulp density at 6.23 N cm-3. Additionally, it resulted in the lowest percentage of anthracnose disease at 7.5% and the longest shelf life of 8 days.
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Paclobutrazol enhances yield and secondary compound accumulation in Curcuma longa L.
(2024-11-01)
Montri, N.
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Chauytam, S.
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Deewatthanawong, R.
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Bunya-Atichart, K.
The results indicated that the application of different concentrations of PBZ at 60 days preharvest had an impact on yield after harvest at 9 months while the color of turmeric remained relatively consistent. In all treatments, the rhizome color was yellow to orange. The fresh weight of the rhizome showed no significant differences within the range of 0-800 mg/L PBZ treatments, with the lowest yield observed in 1,200 mg/L PBZ treatment. The dry weight percentage was highest when treated with 800 mg/L PBZ for the mother rhizome and 600 mg/L PBZ for the finger rhizome. Regarding the accumulation of secondary compounds, highly significant differences among the treatments were found for the content of total flavonoids and total phenolics while curcumin showed significant differences. The treatment with 1,200 mg/L PBZ had the highest total phenolics content at 78.18 mg GAE/gDW and total flavonoids at 295.54 mg QUE/gDW. PBZ-treated plants also showed higher curcumin content and percentage than the non-treated plants. These findings indicated that the application of PBZ has the potential to improve both the yield and quality of turmeric, which is advantageous for cultivating this valuable medicinal plant.
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Application of calcium chloride on yield and quality of Capsicum annuum L.
(2024-09-01)
Montri, N.
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Sengseng, S.
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Deewatthanawong, R.
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Bunya-Atichart, K.
Application of calcium chloride on yield and quality of Capsicum annuum L. 'Super Hot' were investigated. The experiments were conducted in randomized complete block design (RCBD). 0, 20, 40, 60, 80, and 100 mg L-1calcium chloride was sprayed preharvest at the orange-red ripening stage. The ripe red fruits were harvested. Yield and quality in terms of fruit color and secondary compound content were measured. The results showed that spraying with various concentrations of calcium chloride did not affect the percentage of dry weight and color of fruit but significantly influenced fresh and dry weight, capsaicin, total phenolics, and total flavonoid contents. Preharvest spraying of calcium chloride at 40 mg L-1treatment gave the maximum fresh and dry weight and total phenolics at 1,257.83 g, 361.35 g, and 44.75 mg GAE g-1DW, respectively. A high content of total flavonoid at 63.50 and 6.75 mg QE g-1DW was observed in 20 to 40 mg L-1treatment, while high contents of capsaicin contents at 64.32 mg g-1 DW were achieved in 80 mg L-1,.
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Effect of plant growth regulators on in vitro seedling growth of Papilionanthe hookeriana (Rchb.f.) Schltr.
(2023-02-01)
Montri, N.
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Charoensrimuang, S.
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Deewatthanawong, R.
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Montri, W.
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Bunya-Atichart, K.
The effect of plant growth regulators on the in vitro seedling growth of Papilionanthe hookeriana (Rchb.f.) Schltr. was evaluated. The protocorms were cultured in VW media supplemented with combinations of indole acetic acid (IAA) and thidiazuron (TDZ) at the concentrations of 0, 0.1 and 0.2 mg L-1. The 2 leaf stage seedlings were cultured on VW media supplemented with cytokinins; 6-furfurylaminopurine (kinetin: K), benzyladenine (BA) and TDZ or auxins; IAA, indole-3-butyric-acid (IBA), α-naphthalene acetic acid (NAA) at the concentrations of 0, 2 and 4 mg L-1 for 2 months. The results found that combinations of IAA and TDZ had higher protocorm fresh weight when compared with control. Adding with 0.1 and 0.2 mg L-1 IAA alone were promoted protocorm fresh weight. On the other hand, combinations of 0.1 mg L-1 IAA and 0.2 mg L-1 TDZ also improved the fresh weight number. The combinations of TDZ and IAA treatments were achieved for the number of 1st stage protocorm. For 2 leaf stage seedling culture, treated with cytokinins was not significantly different in shoot length, seedling fresh weight and leaf number. However, cytokinins suppressed root number compared to untreated. The maximum root numbers at 4.21 roots were found in free cytokinins medium. Seedling fresh weight and leaf numbers were significantly increased when increased the concentration of auxins. Our results indicated that auxins and cytokinins played an instrumental role for P. hookeriana seedling growth.
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Plant host selection for spore production of Glomas mosseae
(2022-04-01)
Deewatthanawong, R.
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Bannajit, W.
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Deewatthanawong, P.
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Bunya-Atichart, K.
Five species including Ipomoea aquatica, Triticum aestivum, Oryza sativa, Zea mays, and Vigna radiata were used as plant hosts for spore production of Glomas mosseae. Seeds of all plant species were surface sterilized and grown in sterilized growing medium with a ratio of 1 part soil to 8 part sand. Each pot of each plant species was inoculated with 30 spores of G. mosseae and compared with the control without inoculum. Each treatment consisted of five replicates containing four pots. Half-strength Hoagland solution was applied once a week and plant growth parameters including diameter, height, and number of leaves were recorded every week for 2 months. Spores of G. mosseae in the rhizosphere, root colonization, and plant dry mass were measured after 2 months of inoculation. Results showed that G. mosseae improved plant growth of all plant species, with maximum increase in plant diameter found in Z. mays; while, maximum effects on plant height and root dry mass were observed in V. radiata. Results of spore formation suggested Z. mays as the most suitable host for mass spore production with the highest spore numbers of 97.4 100 g-1 soil; however, no significant differences in root colonization were shown among the five plant species.
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Environmental factors affect Postharvest quality of gnetum gnemon L. Leaves
(2013-11-05)
Bunya-Atichart, K.
;
Kangtang, S.
The effects of light intensity, relative humidity and temperature on the quality of Gnetum gnemon L. (melinjo) leaves were studied. The immature melinjo leaves were used in all experiments. The results showed that the leaf color change and shelf life of melinjo leaves stored under normal light or additional light of neon light (light intensity ~13 μmol m-2 s-1) in day time were not significantly different. Melinjo leaves were sprayed with water before packing in polyethylene (PE) bag under low, moderate, and high relative humidity then moving to a stored at room temperature (29°C). It was found that leaves packed in PE bag with moderate relative humidity had the longest shelf life about 17.6 days. Moreover, the effect of temperature on shelf life and quality of melinjo leaves was determined. Melinjo leaves packed in 12 holes-perforated PE bag were stored at 4, 8, 12, 29 and 35°C. The results showed that melinjo leaves storage at 8°C had the longest shelf life (29.4 days) and leaf color did not change to yellow color. The major loss of melinjo leaves quality after harvest and during storage was leaf wilting and decay but not leaf yellowing. In conclusion, the recommendation to keep the postharvest quality of melinjo leaves is to spray leaves with water before packing in perforated PE bag and store at 8°C.

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