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Plant regeneration of Dimocarpus scandens (Winit & Kerr) Boonsuk & Chantar. (Lamyai-Thao) by tissue culture technique
(2025-05-01)
Jitwetkul, A.
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Poeaim, A.
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Pongtongkam, P.
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Chareonsap, P. P.
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Poeaim, S.
The minimum contamination percentage (25%) was recorded in the experiment using 0.2% mercuric chloride (HgCl2) for 20 min, which was observed to be a 50% germination rate after 2 weeks of culture. The maximum germination rate (68%) was obtained on Murashige and Skoog (MS) solid medium supplemented with 1.0 mg/L 6-benzylaminopurine (BAP), giving a shoot length of 48.00 mm. and root length of 31.40 mm. The optimal condition of nodal segment sterilization was recorded in the experiment using 0.1% HgCl2 for 10 min, which gave 80% survival explants. For shoot induction, it was worked on MS solid medium with 0.5 mg/L 6-benzylaminopurine (BAP) that induced the highest average length of 11.31 mm. The highest root formation frequency (50%) and root length (3.0 mm) were achieved on an MS medium containing 2.0 mg/L indole-3-butyric acid (IBA). The plantlets were acclimatized and established in the soil.
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Plant regeneration of Bauhinia purpurea by tissue culture technique
(2024-05-01)
Saparam, W.
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Poeaim, A.
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Pongtongkam, P.
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Chareonsap, P. P.
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Poeaim, S.
Results showed that the seed of Bauhinia purpurea germination was archived 100% from half-strength MS medium supplemented with 3.0 mg/L Gibberellic acids (GA3) and gave 71.137 mm for the highest seedling. The optimal condition of nodal explants sterilization was E5, which gave 92% of survival explants. For the shoot induction method, the results showed that 24.270 mm was the highest proliferation of shoots achieved from MS medium supplemented with 1.0 mg/L 6-benzylaminopurine (BAP). The highest number of roots was 2.867 roots, and the highest root length was 33.453 mm, obtained at half-strength MS medium complemented with 0.25 mg/L Indole-3-acetic acid (IAA). The 73.33% of plantlets were survived after being transferred to plastic pot.
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Effects of growth regulators on in vitro propagation of Sophora tomentosa L. (Necklace pod)
(2024-05-01)
Rungprateeppaiboon, N.
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Chareonsap, P. P.
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Pongtongkam, P.
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Poeaim, A.
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Poeaim, S.
Seeds of Sophora tomentosa were sterilized and germinated on MS medium with BAP (1 mg/L), which was observed to be 75% germination rate with the highest amount of multiple shoots (3 shoots per seed) and an average length of 29.56 mm. The germination rate was reduced to 33.33% by 1% NaCl. Multiple shoot induction was worked on MS medium with 2.0 mg/L of TDZ that induced the highest shoot bud formation rate (46.67%) with the highest amount of shoot bud (10.71 buds per culture), while 1.0 mg/L GA3 showed the highest average length of 34.13 mm. For root induction, 20% rooting was formed in 1/2 MS medium with a combination of 0.5 mg/L IAA and BAP with 0.1% AC, with 3 roots per culture and an average root length of 15.36 mm. After acclimatization for 16 weeks, 70% of the plantlets successfully survived.
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Genetic characterization of cassava (Manihot esculenta Crantz) cultivars using ISSR molecular marker
(2024-05-01)
Poeaim, S.
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Sansanee, S.
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Chantaraprasit, T.
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Chareonsap, P. P.
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Tangthirasunun, N.
The genetic diversity of 22 cassava varieties using the inter-simple sequence repeat (ISSR) molecular marker was investigated. Genetic similarity was derived from the matrix of simple similarity coefficients using the unweighted pair group method with arithmetic average (UPGMA). The genetic similarity coefficient ranged from 0.73 to 0.94. The similarity coefficient at 0.73, cluster analysis was clustered to be 22 cassava varieties into two significant groups. The first group was the sweet variety of cassava used for consumption, consisting of Rayong2, Hana-tee and variegated leaves used for decoration. Another group, the bitter variety of cassava used for industry, consisted of Rayong1, Rayong3, Rayong5, Rayong7, Rayong9, Rayong11, Rayong15, Rayong60, Rayong72, Rayong86-13, Rayong90, Kasetsart50, Kasetsart72, Huay Bong60, Huay Bong80, Huay Bong90, Pirun1, Pirun2 and unknown. The sweet and bitter variety contained different levels of cyanide content. So, the ISSR marker might be able characterize two major cassava varieties.
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Study on optimization for in vitro propagation of Dalbergia oliveri by plant tissue culture
(2024-03-01)
Nonthasila, S.
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Pongtongkam, P.
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Chareonsap, P. P.
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Poeaim, A.
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Poeaim, S.
The experimental findings demonstrated that the highest proliferation frequency (100%), along with the maximum number of shoots per explant (3.33±0.52 shoots) and an average shoot length of 30.04±1.70 mm, were achieved in the Murashige and Skoog (MS) medium supplemented with 2.0 mg/l of 6-benzylaminopurine (BAP). Subsequently, upon reaching a height of 3 to 4 cm, the in vitro micro shoots were transferred to a ¼ MS medium enriched with 0.75 mg/l of indole-3-acetic acid (IAA). This particular regimen resulted in the highest number of roots per shootlet (3.50±0.58) with an average root length of 34.19±0.79 mm. The acclimatization phase of the plantlets to the soil was successful, demonstrating a commendable survival rate of 80%.
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Micropropagation of Ironwood (Xylia xylocarpa (Roxb.) Taub.) by tissue culture
(2024-03-01)
Panudom, S.
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Pongtongkam, P.
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Chareonsap, P. P.
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Poeaim, A.
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Poeaim, S.
It was found that the sterilization procedure was highly effective, resulting in a 100% survival rate of Xylia xylocarpa (Roxb.). After sterilization, the seeds were cultured on a half-strength Murashige and Skoog (MS) medium without adding plant growth regulators (PGRs). A maximum germination percentage of 100% was achieved after 2 weeks of culture. 1.5 cm-sized node segments from both seeds and mother trees were used for shoot induction. These node segments were treated with the same sterilization conditions as the seeds but with the addition of 0.1% HgCl2. The node segments achieved a 90% survival rate despite the additional chemicals. The node segments from seeds were then cultured on a full-strength MS medium supplemented with 0.50 mg/l of BAP. The results showed a maximum number of shoots, reaching 4.10±1.20 per explant and a mean shoot length of 3.87±0.30 cm. The part of node segments derived from mother trees showed 3.50±1.27 shoots per explant, accompanied by a mean shoot length of 3.18±0.89 cm when using BAP concentration of 0.25 mg/l after 12 weeks of cultivation. The rooting medium contained half-strength MS medium supplemented with 0.75 mg/l of IBA. After 8 weeks of being cultured in the rooting medium, the shoots exhibited a maximum rooting percentage of about 80%. The technique provides a valuable method for rapidly propagating and conserving this plant species in a controlled and efficient manner.
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Assessing genetic diversity of Yellow Star tree using SRAP markers and efficacy of their endophytic fungi in biological control
(2023-05-01)
Poeaim, S.
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Tangthirasunun, N.
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Chareonsap, P. P.
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Poeaim, A.
The result showed that 52 fragments (64.22%) were polymorphic bands from seven combinations of Sequence-Related Amplified Polymorphism (SRAP) markers. The genetic relationship was analyzed using the UPGMA method by NTSYpc software version 2.11X showed a similarity coefficient ranging from 0.59 to 0.98. Endophytic fungi were isolated from fresh leaves of Yellow Star (Schoutenia glomerata King subsp. peregrina (Craib) Roekm. & Hartono and identified by morphology and DNA barcoding. The fungi were identified in Alternaria alternata, Colletotrichum siamense, Diaporthe arengae, Phyllosticta capitalensis, and Xylaria cubensis. The dual culture test evaluated the efficacy of endophytic fungi isolated from Yellow Star tree against Colletotrichum gloeosporioides and Phomopsis asparagi causing disease in asparagus. The results revealed that X. cubensis had the potential to inhibit the spore germination of the C. gloeosporioides ASGC06 and P. asparagi ASGP04, which inhibited spore germination of 99.97 and 100 percent, respectively. However, this fungus should be further studied for biological control and bioactive secondary metabolites.
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Identification of phenolic compounds and evaluation of biological activities of methanolic extracts obtained from two varieties of longan (Dimocarpus longan) peels
(2022-01-01)
Chantarasaka, S.
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Chareonsap, P. P.
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Poeaim, S.
Dimocarpus longan belongs to the Sapindaceae family, which are revealed as cryptic species. In Thailand, Dimocarpus longan spp. longan var. longan such as Edor is the most cultivated. Another Dimocarpus longan spp. longan var. obtusus (Dimocarpus obtusus), called Lum-Yai Thao, is found in Eastern Thailand. However, there is still little information on the biological activity of Thao. Hence, this research was designed to identify phenolic compounds and evaluate the biological activities of methanolic extracts obtained from peels of two longan varieties: Edor VS Thao. For the total phenolic compound, no significant differences were observed between Edor and Thao. For antioxidant activity using DPPH, ABTS, and FRAP assays, the analogs showed Edor had more activity than Thao. The disc diffusion test at 5 mg/disc showed that Edor peels extract effectively inhibited the growth of bacteria, but not Thao extracts which were strong against Propionibacterium acnes. However, both of them also did not have activity against Escherichia coli and Staphylococcus epidermidis.For anti-tyrosinase activity, both variety extracts had less effective anti-tyrosinase activity. In addition, anti-inflammatory activity was evaluated by measuring nitric oxide (NO) generated from SNP and using LPSstimulated RAW 264.7 cells which showed excellent activity.In cytotoxicity using MTT assay, the results demonstrated that the methanolic extract of longan peel from Thao exhibited low cytotoxicity against both L929 and HaCaT. On the other hand, the extract from Edor exhibited cytotoxic activity. These results suggested that Thao peel extracts are different from Edor peel extract. Thao peel extracts showed excellent anti-inflammatory and little cytotoxic activity for L929 and HaCaT cell lines and antibacterial against P. acnes. Therefore, Thao peel extracts could be used in preparing products for antiinflammatory supplements and cosmetics.
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The effects of abscisic acid and sorbitol on the slow growth in vitro preservation of Mahesak (Tectona grandis L.)
(2020-09-01)
Tongsad, P.
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Laipasu, P.
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Chareonsap, P. P.
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Poeaim, Anurag
The conservation of teak (Tectona grandis L.) by slow growth was proceeded out to determine appropriate methods in vitro preservation using osmotically active compounds and growth retardants. Calli and node explants were cultured on Murashige and Skoog (MS) and Woody Plant Medium (WPM) media supplemented with 1, 2 and 3 mg. L-1abscisic acid (ABA) containing 30 g.L-1sucrose and hormone-free MS and WPM media containing 0.1, 0.2 and 0.3 M sorbitol for reduced the growth rate. Result showed that preservation of calli on MS and WPM media supplemented with 0.2 and 0.3 M sorbitol and node explants were cultured on WPM medium supplemented with 0.2 and 0.3 M sorbitol inhibited the growth and maintained the quality of calli and node explants for 12 weeks. It was found that ABA could not inhibit the growth rate after cultures for 12 weeks. Callus and node explant survival from slow growth preservation were able to regrowth which successfully (100%) tested on WPM medium supplemented with 0.25 mg.L-16-benzylaminopurine (BAP) containing 30 g.L-1sucrose after for 2 weeks.
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The effect of plant growth regulator and in vitro conservation of teak (Tectona grandis L.) by tissue culture
(2018-12-01)
Tongsad, P.
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Laipasu, P.
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Chareonsap, P. P.
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Poeaim, A.
The plant regeneration of teak (Tectona grandis L.) was reported. Result showed that the highest number of shoot induction was 55.49 cm., in length of shoots which tested at 0.25 mg·L -1 BAP, and averaged 40.29 cm which tested at 0.25 mg·L -1 mT after 4 weeks. The shoots were elongated using shoot tip explants of cryopreservation on teak by encapsulation-dehydration (EnDe), encapsulation-vitrification (EnVi) and vitrification (Vi) techniques. The shoot tips were used as explants, encapsulated apices were precultured on hormone-free MS medium containing 0.3 M sucrose for 2 days, dehydrated, and immersed in liquid nitrogen for 24 h after that thawed in thermal bath at 40 ºC and washed in unloading solution. Vi and EnVi protocols were used to treat with a loading solution, dehydrated by plant vitrification solution 2 (PVS2). EnDe protocol were encapsulated by desiccated air-dry in laminar air flow and dehydrated with silica gel. Results showed that Vi gave an excellent shoot tip explants for survival and regrowth. Vi were unable to survival. This experiment was preliminary searched to improve and develop the teak for cryopreservation.

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