KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/123456789/1

Browse

Settings

Author: search.filters.author.Chareonsap, P. P.Subject: search.filters.subject.Dimocarpus longan

Search Results

Now showing 1 - 2 of 2
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Identification of phenolic compounds and evaluation of biological activities of methanolic extracts obtained from two varieties of longan (Dimocarpus longan) peels
    (2022-01-01)
    Chantarasaka, S.
    ;
    Chareonsap, P. P.
    ;
    Poeaim, S.
    Dimocarpus longan belongs to the Sapindaceae family, which are revealed as cryptic species. In Thailand, Dimocarpus longan spp. longan var. longan such as Edor is the most cultivated. Another Dimocarpus longan spp. longan var. obtusus (Dimocarpus obtusus), called Lum-Yai Thao, is found in Eastern Thailand. However, there is still little information on the biological activity of Thao. Hence, this research was designed to identify phenolic compounds and evaluate the biological activities of methanolic extracts obtained from peels of two longan varieties: Edor VS Thao. For the total phenolic compound, no significant differences were observed between Edor and Thao. For antioxidant activity using DPPH, ABTS, and FRAP assays, the analogs showed Edor had more activity than Thao. The disc diffusion test at 5 mg/disc showed that Edor peels extract effectively inhibited the growth of bacteria, but not Thao extracts which were strong against Propionibacterium acnes. However, both of them also did not have activity against Escherichia coli and Staphylococcus epidermidis.For anti-tyrosinase activity, both variety extracts had less effective anti-tyrosinase activity. In addition, anti-inflammatory activity was evaluated by measuring nitric oxide (NO) generated from SNP and using LPSstimulated RAW 264.7 cells which showed excellent activity.In cytotoxicity using MTT assay, the results demonstrated that the methanolic extract of longan peel from Thao exhibited low cytotoxicity against both L929 and HaCaT. On the other hand, the extract from Edor exhibited cytotoxic activity. These results suggested that Thao peel extracts are different from Edor peel extract. Thao peel extracts showed excellent anti-inflammatory and little cytotoxic activity for L929 and HaCaT cell lines and antibacterial against P. acnes. Therefore, Thao peel extracts could be used in preparing products for antiinflammatory supplements and cosmetics.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Biological activities of the methanolic extracts from two varieties of Dimocarpus longan seeds
    (2018-12-01)
    Natungnuy, K.
    ;
    Chareonsap, P. P.
    ;
    Poeaim, S.
    Dimocarpus longan belongs to Sapindaceae which two varieties are found in Thailand. In addition to commercial longan (D. longan ssp. longan var. longan), in the eastern part of Thailand found another type of flora longan called Thao (D. longan ssp. longan var. obtusus). The total phenolic content and various biological activities was investigated including antioxidant, antibacterial, anti-tyrosinase and cytotoxic activities of the methanolic extracts from two varieties of these longan seeds. The results showed that the most activity of Edor (var. longan) seed extract presented higher biological activities than Thao (var. obtusus) seed extract. The total phenolic content by Folin-Ciocalteu method related to antioxidant activity in DPPH, ABTS and FRAP assays. In antibacterial activity using disc diffusion method both varieties of extracts (5 mg/disc) inhibited Staphylococcus aureus, Micrococcus luteus and Bacillus subtilis. However, in anti-tyrosinase activity by the dopachrome method Thao revealed similar activity to the commercial longan cultivar Edor with 50% inhibitory concentration (IC <inf>50</inf> ) values of 504.73 and 527.04 µg/ml, respectively. In cytotoxic activity using MTT assay with L929 (Mouse fibroblast cell line), Thao seed extracts were non-cytotoxic activity and stimulated skin cell lines, while Edor showed low cytotoxic activity. Therefore, their bioactive compounds should be studied further for developing longan seed extracts to cosmetic and pharmaceutical products in the future.