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Author: search.filters.author.Charunanthasunthon, Worasan

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    Functional and genomic characterization of Pediococcus pentosaceus NR4-4 reveals a safe bacteriocinogenic probiotic with strong anti-listerial activity and prebiotic metabolism
    (2026-05-01)
    Ratthanachot, Natnarin
    ;
    Woraprayote, Weerapong
    ;
    Janyaphisan, Thitiphorn
    ;
    Tanasupawat, Somboon
    ;
    Ochaikul, Duangjai
    The growing interest in bacteriocin-producing lactic acid bacteria from traditional fermented foods has highlighted the value of strains that combine antimicrobial activity, probiotic functionality, and genome-supported safety. This study presents an integrated functional and probiogenomic characterization of Pediococcus pentosaceus NR4-4 isolated from Thai fermented mustard greens. The strain was found to produce a heat-stable class IIa bacteriocin with pronounced inhibitory activity against Listeria monocytogenes . In addition, NR4-4 exhibited strong tolerance to simulated gastrointestinal conditions and demonstrated effective adhesion to Caco-2 intestinal epithelial cells. In vitro safety evaluations confirmed that the strain was non-hemolytic and did not exhibit cytotoxic effects toward AGS or Caco-2 cell lines. Whole-genome sequencing verified species identity and revealed the presence of pediocin-related gene clusters, carbohydrate-active enzymes associated with fructooligosaccharide utilization, and a favorable genome-based safety profile. Notably, no virulence factors, biogenic amine biosynthesis pathways, plasmids, or transferable antimicrobial resistance determinants were detected. The preferential metabolism of fructooligosaccharides further suggests compatibility with synbiotic formulations. Overall, these findings indicate that P. pentosaceus NR4-4 is a genomically supported, safe, and multifunctional probiotic candidate with potential applications in food biopreservation and functional food development.
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    Antioxidant Activity, γ-Aminobutyric Acid, and Genome Analysis of Lactiplantibacillus plantarum FL13-2 from Fermented Rice Flour
    (2025-09-01)
    Songsumanus, Apakorn
    ;
    Pinyosnit, Natawadee
    ;
    Kingkaew, Engkarat
    ;
    Panngeun, Thanapol
    ;
    Charunanthasunthon, Worasan
    Ten rod-shaped isolates of lactic acid bacteria (LAB) from the traditional fermented rice flour, khao-khab were identified as belonging to the genus Lactobacillus based on phenotypic characteristics. Group I isolates (FL12-1, FL18-1, FL19-1S, FL23-1, FL24-1, FL25-1, and FL26-1) were closely related to Limosilactobacillus fermentum, exhibiting 16S rRNA gene sequence similarity of 99.4%–100%. Group II included FL13-2 and FL22-2 (Group IIA) and FL17B (Group IIB), closely related to Lactiplantibacillus plantarum (99.9%–100%) and Lactiplantibacillus pentosus (100%), respectively. All isolates demonstrated strong antioxidant potential, with 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity—measured by the standard DPPH assay—ranging from 78.49±1.01% to 91.18±3.95%, surpassing that of ascorbic acid. Strain FL13-2, identified as L. plantarum through genomic analysis (Average Nucleotide Identity (ANI) 98.5%, digital DNA-DNA hybridization (dDDH) 92.5%), produced 2.43 g/L of γ-aminobutyric acid (GABA). It harbored key biosynthesis and transport genes (gadB, gadC, gatABCD, pdxK). Phylogenetic analysis confirmed high sequence similarity of these genes with other LAB strains, supporting their functional roles. The gad system was implicated in acid resistance and GABA production under low pH conditions. Genome annotation revealed bacteriocin genes (Enterocin X β, Plantaricin E, and F) and indicated low pathogenicity and absence of virulence factors. Antimicrobial resistance genes (vanH, vanT, vanY) were detected; however, these are intrinsic to LAB and associated with essential cell wall biosynthesis rather than acquired resistance. Additionally, the presence of the qacJ efflux pump and hlyIII gene—both commonly found in probiotic strains—further supports the safety profile of FL13-2. These findingshighlight L. plantarum FL13-2 as a promising multifunctional probiotic candidate with potent antioxidative, GABA-producing, and antimicrobial properties.
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