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Author: search.filters.author.Chayaprasert, Wilailack

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    Plant physiological impacts and flavonoid metabolic responses to uptake TiO2 nanoparticles
    (2020-04-01)
    Sompornpailin, Kanokporn
    ;
    Chayaprasert, Wilailack
    The presence of Titanium dioxide nanoparticles (TiO<inf>2</inf> NPs) contamination in the environment is of concern because of their oxidative effect on organisms. Transgenic tobaccos that overexpressed PAP1, a MYB75 gene, were used to investigate the response of plants to the presence of TiO<inf>2</inf> NPs and the results were compared to those for wild type (WT) plants. The experiment was performed under tissue culture conditions with daylight fluorescence. The physiological responses of the plants under moderately low concentrations of TiO<inf>2</inf> NPs (20-40 mg L<sup>-1</sup>) were analyzed in relation to metabolic responses and the results were compared to those of plants under zero TiO<inf>2</inf> conditions. Under conditions of 20 mg L<sup>-1</sup> TiO<inf>2</inf>, WT and PAP1 plants showed better physiology than plants under other conditions. These plants had higher chlorophyll and carotenoid levels, and better membrane stability than plants under non TiO<inf>2</inf> conditions. The WT plants grown in medium at 40 mg L<sup>-1</sup> TiO<inf>2</inf> showed deteriorated physiology, while PAP1 plants grown under the same condition were shown various changes in physiology depending on the line. Moreover, the content of total soluble sugar (TSS) and flavonoids in the extracts of plant were increased in response to the concentrations of TiO<inf>2</inf>. However, all PAP1 transgenics had flavone and flavonol contents that were approximately 2-3 times the levels found in WT plants, while TSS and anthocyanin subgroup levels were not different among the WT and transgenic plants. Excessive nanoparticles can induce oxidative damage in cells, and it appears that PAP1 transgenics can alleviate such damage by enhancing flavonoid accumulation.
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    Effects of modulated concentration of ZnO nanoparticles on enhancing biosynthesis of metabolites and protecting plant membrane
    (2019-01-01)
    Chayaprasert, Wilailack
    ;
    Sompornpailin, Kanokporn
    Nowadays, nanoparticles are raising their applications around the world. ZnO nanoparticles are extensively used in the industrial and agricultural processes because of low price and less toxicity. Nevertheless, the pros and cons, effects of ZnO nanoparticles are still under discussing. This research aimed to study the protective effects of ZnO nanoparticles on plant physiology at the cellular level by analyzing biomaterial levels in relation to the stability of cellular membranes. Shoots of wild type and tt8 transgenic tobaccos were grown under tissue culture condition in the medium, adding 0, 10, 20 mg/L ZnO nanoparticles. Under media conditions adding 10, 20 mg/L ZnO nanoparticles, tobacco increased accumulation of plant metabolites (total soluble sugar and flavonoids). In these ZnO conditions, wild type and transgenic tobaccos enhanced total soluble sugar about 30-50% and 20-30%, respectively. The highest content of total soluble sugar was found in 20 mg/L ZnO nanoparticle condition. In similarity to the content of total soluble sugar, tobacco plants showed the highest average contents of flavone, flavonol and anthocyanin after growing in 20 mg/L ZnO nanoparticle conditions, 92.8%, 61.39% and 48.81% in wild type and 73.49%, 52.39% and 81.68% in transgenics, respectively. Tobaccos under 10 mg/L ZnO media condition increased the accumulation of total soluble sugar and flavonoid contents less than those under 20 mg/L ZnO media condition. However, these plants showed the lowest average percentage of cell membrane injury, 19.5% in wild type and 10-18% in transgenic tobaccos, following with plant under 20 mg/L ZnO nanoparticle and non ZnO nanoparticle conditions. Therefore, 10-20 mg/L ZnO nanoparticles in the media showed the induction of plant metabolites, especially in transgenics, and the enhancements of plant protection when compared with the same line tobacco under condition of non ZnO nanoparticles. Ten mg/L ZnO nanoparticle media is the best condition in protecting cellular membrane of tobacco.