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Author: search.filters.author.Chysirichote, TeerinSubject: search.filters.subject.Centella asiatica

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    pH-driven mechanistic segmentation and enzyme-assisted deconstruction for the selective release of centelloids from Centella asiatica
    (2026-05-01)
    Chysirichote, Teerin
    ;
    Aroonsong, Soysruang
    The effect of pre-extraction treatment of Centella asiatica (L.) using different pre-treatment parameters, namely, pH (4.0–6.0) and treatment time (15–105 min), in combination with enzymatic pretreatment, on centelloid extracts obtained by ultrasound-assisted extraction was investigated. It was observed that the pre-extraction treatment of plant samples at all tested pH values in combination with enzymatic deconstruction led to distinct synergistic effects. Liberation of centelloids was found to occur mainly at two different pretreatment regimes, namely, Phase I (Proton-induced pectin relaxation and rapid glycoside liberation), which led to maximum glycoside yield at pH 4.0 within 15 min (18.20 ± 0.15 mg/g madecassoside and 8.51 ± 0.15 mg/g asiaticoside), and Phase II (Enzyme-mediated collapse and aglycone re-adsorption), in which, at pH 5.0–6.0, the role of enzymatic contribution in enhancing the extraction of aglycones was found to be negligible. Optimum yields and quality of centelloid aglycones were achieved at pH 6.0, where maximal yields were obtained at 60 min and declined thereafter. The observed decline in centelloid aglycone yields at prolonged treatment time could be attributed to the extensive enzymatic fragmentation and subsequent collapse of the disrupted plant matrix, leading to non-specific re-adsorption of the bioactive triterpenes. This study provides a sustainable and cost-effective framework for optimizing extract composition through precise pH and temporal control, offering an efficient methodology for the green separation of bioactive centelloids.
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    Optimization of ethanolic extraction of asiaticoside and asiatic acid: A stability study and correlation of dielectric constant
    (2025-06-01)
    Chysirichote, Teerin
    ;
    Wattanasiriwit, Waewwan
    ;
    Uraipong, Chatchaporn
    This study optimized the extraction of asiaticoside and asiatic acid from Centella asiatica using an ultrasonic-assisted extraction method. The research focused on ethanol concentration, extraction temperature, and extraction duration to maximize yield efficiency. In addition, the stability of the extracted compounds was assessed under different temperature and pH conditions for practical applications. The highest asiaticoside yield was achieved using 53.7 % ethanol at 50.0 °C for 35 min., whereas asiatic acid extraction was most efficient using 63.3 % ethanol at 64.3 °C for 28 min. A significant correlation was observed between the dielectric constant of the extraction conditions, which was affected by solvent concentration and temperature, and extraction efficiency. Stability tests showed that temperature had a more pronounced impact than pH, with degradation accelerating at higher temperatures. The findings emphasize the importance of maintaining processing conditions below 70 °C and within an acidic to neutral pH range. These results provide valuable insights for industrial applications in pharmaceutical, cosmetic, and nutraceutical sectors, underscoring the necessity of controlled conditions to preserve the bioactive properties of Centella asiatica extracts.