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Author: search.filters.author.Deewatthanawong, R.Subject: search.filters.subject.Secondary metabolite

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    Effect of calcium phosphate on growth, pigment and secondary compound accumulation in Cannabis sativa L. callus
    (2026-05-01)
    Tebdoie, C.
    ;
    Thipphaaut, T.
    ;
    Deewatthanawong, R.
    ;
    Kongchinda, P.
    ;
    Singhavorachai, P.
    The application of calcium phosphate in the form of tricalcium phosphate (Ca3(PO4)2) on cannabis (Cannabis sativa L.) callus cultures for 7 days significantly increased fresh and dry weights at concentrations ranging from 800 to 2000 mg/L, with no statistical differences between concentrations. However, higher calcium phosphate concentrations led to reductions in pigments and DPPH radical scavenging activity. By contrast, callus cultures treated with 800 and 1600 mg/L showed enhanced total phenolic content and carotenoids, while total flavonoid content increased proportionally. These findings suggested that calcium phosphate concentration influenced the biosynthesis of secondary metabolites in cannabis callus cultures.
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    GC-MS analysis and biopesticide properties of different crude extracts of Annona squamosa and Annona muricata
    (2019-01-01)
    Deewatthanawong, R.
    ;
    Kongchinda, P.
    ;
    Deewatthanawong, P.
    ;
    Pumnuan, J.
    ;
    Insung, A.
    Most common compounds identified by GC-MS analysis of ethanolic extracts from leaves of Annona squamosa (sweetsop) and Annona muricata (soursop) were neophytadiene, hexadecanoic acid, and trans-caryophyllene. Hexadecenoic acid and ethyl oleate were identified in seeds of A. muricata, while only methyl-p-tert-butyl phenylacetate was identified in seeds extract of A. squamosa. Crude extracts were tested for their antifungal activities against postharvest plant pathogens, insecticidal activities against brown planthopper, Nilaparvata lugens (Stål) and cassava mealybug, Phenacoccus manihoti Matile-Ferrero. Results showed low inhibitory effect (less than 20%) for the pathogenic fungi insecticidal test against cassava mealybug of all crude brown planthopper treated with 5% seed extract of A. squamosa, indicating its potential as an alternative botanical insecticide. Further studies are required to improve yield and extract quality.