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Author: search.filters.author.Eiad-ua, ApiluckSubject: search.filters.subject.Ultrasound

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    Ultrasonic enhancement of lipase-catalyzed transesterification for biodiesel production from used cooking oil
    (2023-06-01)
    Patchimpet, Jaran
    ;
    Zhang, Yi
    ;
    Simpson, Benjamin K.
    ;
    Rui, Xin
    ;
    Sangkharak, Kanokphorn
    This work investigated the ultrasonic irradiation-enhanced transesterification using lipase from the viscera of Nile tilapia (Oreochromis niloticus) to produce biodiesel from used cooking oil (UCO) and methanol. The effects of processing conditions such as irradiation time, ultrasonic frequency, and temperature on biodiesel yield were investigated. Results showed that the use of ultrasound decreased the reaction time from 28 to 3 h with ultrasonic frequency of 16 kHz, methanol to oil molar ratio of 4:1, lipase concentration of 30 kUnit, and reaction temperature of 40 °C. The efficacy of ultrasound was compared with conventional mechanical stirring operated at optimum conditions, and the ultrasonic irradiation coupled with stirring enhanced the transesterification with the highest yield of 97.59%. The fuel properties of the produced biodiesel in this study desirably met the recommended biodiesel standards as prescribed by EN 14214 and ASTM D 6751, suggesting the biodiesel obtained from ultrasound-assisted lipase-catalyzed transesterification of UCO with methanol is a promising alternative for conventional biodiesels and diesels. Graphical abstract: [Figure not available: see fulltext.].
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    Thermoseparating aqueous two-phase system for lipase recovery and partitioning from Nile tilapia viscera: Biochemical properties and effect of ultrasound
    (2021-06-01)
    Patchimpet, Jaran
    ;
    Sangkharak, Kanokphorn
    ;
    Eiad-ua, Apiluck
    ;
    Klomklao, Sappasith
    Lipase from Nile tilapia (Oreochromis niloticus) viscera were partitioned and recovered using a thermoseparating aqueous two-phase system (T-ATPS). Different phase partitioning parameters, including type and concentration of salts, concentration of EOPO, NaCl addition, the EOPO phase/distilled water ratio and temperature were optimized. In the primary ATPS, lipase was satisfactorily partitioned to the EOPO-rich top phase in the optimum system composed of 20% crude enzyme, 40% EOPO 3900, 10% (NH<inf>4</inf>)<inf>2</inf>SO<inf>4</inf> and 4% NaCl. In the secondary ATPS, the optimum ratio between the EOPO-rich top phase and distilled water was 1:1 (w/w) and the optimum temperature for inducing phase separation was 60 °C. After thermoseparation, water solution containing lipase and EOPO were formed in the top and bottom phase of this step, respectively. Under the optimal partitioning condition, the yield (64.45%) and purification fold (PF: 6.30-fold) were obtained. Additionally, the lipases retained in the salt-rich bottom phase in the primary ATPS were successfully recovered from the recycling step no more than three times. The total yield of 93.59% was obtained from this separation system. The partitioned enzyme exhibited optimal activity at pH 8.5 and 40 °C and was stable at a temperature range of 0–40 °C and a pH range of 8–10. It showed high tolerance in the presence of ethanol and methanol. The effect of ultrasound on the partitioned lipase activity was also studied. The highest lipase activity was achieved when the sample was treated with ultrasound at 180 W and 24 kHz for 20 min at 40 °C, under which the activity was increased by 110.73% over the control. Therefore, the T-ATPS was found to be an attractive technique for the recovery and partial purification of lipase from Nile tilapia viscera. Also, ultrasound could obviously improve its activity.