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Author: search.filters.author.Gao, XinghuaHas files: No

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    Ultrathin Cu-TCPP Nanosheet-Based Electrochemical Microsensor for Detecting the Immunosuppressive Drug Mycophenolic Acid
    (2023-12-22)
    Deng, Mingshi
    ;
    Jin, Wei
    ;
    Yang, Wenjuan
    ;
    Tian, Lingling
    ;
    Gao, Xinghua
    Mycophenolic acid (MPA), which is a potent immunosuppressant, has been widely used to prevent organ rejection after organ transplantation. In clinical practice, it is essential to monitor the free MPA concentration. Herein, convenient and fast electrochemical microsensing technology is developed for directly detecting the MPA concentration. To implement this method, ultrathin copper-based metal-organic framework (Cu-MOF) nanosheets with a thickness of about 6.6 nm are used to detect MPA. An analysis of electrochemical behavior reveals the direct electrochemical catalytic oxidation mechanism of MPA molecules on a Cu(II) tetrakis(4-carboxyphenyl)porphyrin (Cu-TCPP)-modified electrode. Furthermore, electrochemical testing results show that the ultrathin Cu-TCPP nanosheet-based microsensor exhibits high sensitivity and specificity for MPA in the nano- to micromolar range, with a detection limit of 10 nM. X-ray absorption spectroscopy (XAS) characterization reveals that the unsaturated metal ions in ultrathin nanosheets are the active sites responsible for boosting the catalytic oxidation of MPA molecules. High-performance liquid chromatography-tandem mass spectrometry further shows the catalytic oxidation product of MPA and that the phenolic hydroxyl group in the MPA molecule is the active site where the catalytic reaction occurs. The considerable sensing performance of the Cu-TCPP nanosheet-based microsensor proves that it is possible to develop a fast and convenient method for the direct high-frequency monitoring of immunosuppressive drugs.
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    RhIr@MoS2 nanohybrids based disposable microsensor for the point-of-care testing of NADH in real human serum
    (2020-08-01)
    Ji, Dongqing
    ;
    Ying, Zi
    ;
    Zhang, Yuan
    ;
    Chen, Wei
    ;
    Janyasupab, Metini
    Dihydronicotinamide adenine dinucleotide (NADH) is an important enzyme in all living cells, which is found to be abnormally expressed in cancer cells. Since it is redox-active, an electrochemical detection method would be suitable for monitoring its concentration in biological fluids. Here we present a strategy for specific determination of NADH in real human serum by using RhIr@MoS<inf>2</inf> nanohybrids based microsensor. To implement the protocol, RhIr nanocrysrals are in-situ grown onto MoS<inf>2</inf> interlayers forming a nanohybrid structure (RhIr@MoS<inf>2</inf>). After being locally deposited on an electrochemical microsensor, it could be used for the analysis of NADH. The developed RhIr@MoS<inf>2</inf> nanohybrids based microsensor possesses the ability for analyzing NADH at the applied potential of 0.07 V (much lower than most reported values). The detection limit is evaluated as low as 1 nmol/L even in bovine serum albumin (BSA) media. In addition, the sampling analysis of human serum from cancer patients and health controls shows that the microsensor displays good diagnostic sensitivity and specificity, illustrating that this developed detection technique is a relatively accurate method for measuring NADH in biological fluids. The proposed electrochemical microsensor assay also owns the benefits of convenience, disposable and easy processing, which make it a great possibility for future point-of-care cancer diagnosis.