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Author: search.filters.author.Grandjean, FredericSubject: search.filters.subject.Wolbachia

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    Item type:Publication,
    Distribution of Wolbachia infection in butterflies (Lepidoptera): First systematic report from Thailand
    (2023-01-01)
    Boonsit, Panukorn
    ;
    Wiwatanaratanabutr, Itsanun
    ;
    Ross, Perran A
    ;
    Grandjean, Frederic
    ;
    Austin, Chris
    Wolbachia is a genus of Rickettsia-like bacteria that infects a broad range of insect species throughout the world. It often influences host reproduction to facilitate its transmission to their offspring and spread through populations. The occurrence and ecology of Wolbachia has not been surveyed in detail in Thai butterflies. In this study, we conduct the first systematic survey of Wolbachia infection by sampling 623 specimens from 46 butterfly species from 5 families obtained from tropical forests in 3 geographic regions of Thailand. Wolbachia infections were detected using three PCR primer sets: 16S rRNA, ftsZ and wsp. The results showed evidence for widespread Wolbachia infection in Thai butterflies. The Wolbachia presence was confirmed in 291 individuals from 17 butterfly species of 2 families, comprising 86 individuals from the Central Region (32 males and 54 females), 72 individuals from the Northeast Region (25 males and 47 females), and 133 individuals from the West Region (46 males and 87 females). This report will be useful for understanding the distribution of Wolbachia in butterflies from Thailand.
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    Impacts of temperature and crowding on sex ratio, fecundity and Wolbachia infection intensity in the copepod, Mesocyclops thermocyclopoides
    (2016-11-01)
    Wiwatanaratanabutr, Itsanun
    ;
    Grandjean, Frederic
    Wolbachia are a group of intracellular bacteria that cause reproductive alterations in arthropods. Here, we describe the effects of two environmental factors (crowding and temperature) on phenotypic expression of feminization, the host's fecundity and Wolbachia infection intensity among life cycle stages in the naturally Wolbachia-infected copepod, Mesocyclops thermocyclopoides. The copepod was first found to be co-infected with Wolbachia A- and B-supergroups Wolbachia strains based on wsp primers. The relative Wolbachia infection intensity within individuals was determined using quantitative real-time PCR and was significantly higher in the B-supergroup than in the A-supergroup. Experimental results of temperature effect on bacterial density in each developmental stage revealed a significant decrease in Wolbachia infection intensity following exposure to high temperature (37 °C) in both sexes and implied that Wolbachia might survive in room temperature (25 °C) better than in high temperature. Experimental results of crowding effects on Wolbachia infection intensity suggested a negative correlation between copepod nauplii and Wolbachia infection intensity. No effect of rearing temperature on the sex ratio was reported although the fecundity was significantly decreased by high temperature. The results showed that Wolbachia infection intensity to be correlated with crowding conditions and was decreased following exposure of elevated temperature.