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Author: search.filters.author.Hong, Jeong HwaHas files: No

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    Production of fiber hydrolysate from bamboo shoot with antioxidative properties by enzymatic hydrolysis
    (2019-06-04)
    Karnjanapratum, Supatra
    ;
    Kaewthong, Pensiri
    ;
    Takeungwongtrakul, Sirima
    ;
    Sae-Leaw, Thanasak
    ;
    Hong, Jeong Hwa
    Bamboo shoots possess a rich source of dietary fiber for Asian countries as well as Thailand, which give various beneficial physiological effects for human beings. Dietary fibers in soluble form could provide better texture and would be easier to apply in food products. This study aimed to prepare fiber hydrolysate with high solubility and antioxidant activity from bamboo (Bambusa vulgaris) shoot. The fiber hydrolysate from bamboo shoot (FHBS) was prepared by stepwise enzymatic hydrolysis including amylase (1%, w/w), cellulase (1, 2, 3%, w/w) and papain (1%, w/w). The released fiber yield of FHBS increased with increasing cellulose levels in dose dependent manner (P≤0.05). It was found that the process with 1% (w/w) amylase for 1 h and 3% (w/w) cellulase for 3 h followed by 1% (w/w) papain for 1 h at 50 ºC, rendered the highest released fiber yield (92.10±1.10%). The resultant FHBS contained 5.76±0.21 % of total dietary fiber with total sugar and reducing sugar contents of 1431.22±46.01 and 918.91±10.57 mg/g solid, respectively. The FHBS exhibited antioxidant activities including ABTS radical scavenging activities (ABTS), DPPH radical scavenging activities (DPPH), ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC). Therefore, FHBS with antioxidant activities could be effectively prepared by using enzymatic hydrolysis and suitable to apply in the fiber fortified products.
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    Evaluation of antioxidant activities, total phenolic content (TPC), and total catechin content (TCC) of 10 sugar apple (Annona squamosa L.) cultivar peels grown in Thailand
    (2018-12-01)
    Manochai, Benya
    ;
    Ingkasupart, Pajaree
    ;
    Lee, Sang Hyun
    ;
    Hong, Jeong Hwa
    Antioxidant activities, total phenolic content (TPC), and total catechin content were analyzed in the ethanol extracts of 10 sugar apple (Annona squamosa L.) cultivar peels grown in Thailand. Antioxidant activity assays carried out in the this study were the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric ion reducing antioxidant power (FRAP), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation scavenging activity, and oxygen radical absorbance activity (ORAC). ‘Nhur Thong’, ‘Nung Keaw’, and ‘Fai Keaw’ showed high percentage fruit peel (over 38.0%). ‘Nhur Thong’, ‘Nung Keaw’, and ‘Fai Keaw’ ethanol extracts possessed high antioxidant capacities in the majority of methods used, and thus could be potential candidate of natural antioxidants. ‘Fai Keaw’ showed the best activity in the ABTS (1.57 mmol of trolox/g dry sample), FRAP (0.43 mmol of trolox/g dry sample), and ORAC assays (2.84 mmol of trolox/g dry sample), as well as the highest content of TPC (140.4 mg) per gram dry sugar apple peel among the 10 cultivars. TPC showed a positive correlation compared with all antioxidant activity assays. In conclusion, ‘Fai Keaw’ could be utilized as an excellent phenolic compound source for functional food products.
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    β-glucosidase producing Bacillus isolated from Thua-nao, an indigenous fermented soybean food in Thailand
    (2017-10-01)
    Meannui, Nutwara
    ;
    Riebroy, Siriporn
    ;
    Tangwatcharin, Pussadee
    ;
    Hong, Jeong Hwa
    ;
    Sumpavapol, Punnanee
    The characteristics of commercial dried flatten Thua-nao collected from Northern of Thailand, namely oven dried (LPO) and sun dried (LPS) samples from Lamphun, sun dried sample from Chiang Mai (CMS) and sun dried sample from Chiang Rai (CRS),were investigated. The variations of chemical and physical characteristics were found among these Thua-nao products. Seventeen isolates of spore-forming bacteria were obtained from such products. Among these, four isolates (LPO-2, LPO-4, LPS-2 and CMS-1) were identified as Bacillus subtilis group by API 50CHB kit. For cellulolytic enzyme production, CMS-1 exhibited the highest clear zone on tryptic soy agar supplemented with carboxymethyl cellulose (P<0.05). Contrastingly, LPO-2 had the highest β-glucosidase activity with the lowest clear zone of cellulolytic enzyme activity (P<0.05). From 16S rRNA gene sequencing result, LPO-2 could be identified as Bacillus sp. and closely related to B. subtilis group.