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Author: search.filters.author.Hoondee, PatcharapornSubject: search.filters.subject.Astaxanthin

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    Enhancement of astaxanthin production in novel red yeast Rhodosporidiobolus sp. SP3-3/4 and genomic characterization
    (2025-10-01)
    Phuengjayaem, Sukanya
    ;
    Kingkaew, Engkarat
    ;
    Hoondee, Patcharaporn
    ;
    Tanasupawat, Somboon
    Astaxanthin is a red carotenoid and potent antioxidant with anti-inflammatory, anti-cancer, neuroprotective, and immuno-enhancing benefits. This study focused on enhancing astaxanthin production using a novel red yeast, strain SP3-3/4, through genomic analysis. Strain SP3-3/4 shared 99.66% sequence identity in the D1/D2 domain of the large subunit rRNA gene with Rhodosporidiobolus ruineniae CBS 5001<sup>T</sup>. Whole-genome analysis indicated 82.54% similarity to R. ruineniae JCM 8097<sup>T</sup>, with average nucleotide identity (ANI) values below 80% for other strains. The ANI result, lower than the 95% cutoff, confirms SP3-3/4 as a novel species, Rhodosporidiobolus sp. SP3-3/4. Key astaxanthin synthesis genes, including CrtE, CrtYB, CrtI, CrtS, CrtR, CrtW, CrtO, and CrtZ, were annotated. Astaxanthin production was verified using HPLC and LC-MS. Optimization of medium composition and physical parameters identified optimal conditions as 20 g/L sucrose, 3 g/L yeast extract, and 5 g/L peptone, with an initial medium pH of 6.5. Maximum cell growth and astaxanthin content, reaching 9.615 g/L and 0.46 mg/g DCW, respectively, were achieved after 5 days of cultivation, corresponding to an astaxanthin yield of 6.93 mg/L and a productivity rate of 1.39 mg/L/day. Notably, a maximum productivity of 1.45 mg/L/day occurred in 3 days. These findings elucidated comprehensive optimization of astaxanthin production, incorporating functional genomic assessment and analysis of protein-encoded genes of astaxanthin-producing yeast Rhodosporidiobolus sp. SP3-3/4. Such insights represent valuable biological resources with significant implications for diverse biotechnological and bioinformatics applications.
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    Item type:Publication,
    Diversity, astaxanthin production, and genomic analysis of Rhodotorula paludigena SP9-15
    (2023-07-01)
    Phuengjayaem, Sukanya
    ;
    Kingkaew, Engkarat
    ;
    Hoondee, Patcharaporn
    ;
    Rojsitthisak, Pornchai
    ;
    Sritularak, Boonchoo
    Astaxanthin is a carotenoid known for its powerful antioxidant properties. This study focused on isolating yeast strains capable of producing astaxanthin from flower and fruit samples collected in Thailand. Out of 115 isolates, 11 strains were identified that produced astaxanthin. Molecular identification techniques revealed that these isolates belonged to two species: Rhodotorula paludigena (5 isolates) and Rhodosporidiobolus ruineniae (6 isolates). Whole-genome analysis of one representative strain, R. paludigena SP9-15, identified putative candidate astaxanthin synthesis-associated genes, such as CrtE, CrtYB, CrtI, CrtS, CrtR, CrtW, CrtO, and CrtZ. High-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) confirmed astaxanthin production. Further optimization of astaxanthin production was carried out by investigating the effects of various factors on the growth rate and astaxanthin production. The optimal conditions were 40 g/L glucose as a carbon source, pH 7.5, and cultivation at 25 °C with 200 rpm for 3 days. Under these conditions, R. paludigena SP9-15 synthesized biomass of 11.771 ± 0.003 g/L, resulting in astaxanthin with a content of 0.558 ± 0.018 mg/g DCW (dry cell weight), an astaxanthin yield of 6.565 ± 0.238 mg/L, and astaxanthin productivity of 2.188 ± 0.069 g/L/day. These findings provide insights into astaxanthin production using red yeast strains from Thailand and highlight the potential of R. paludigena SP9-15 for further application.