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Author: search.filters.author.Hunsub, Panusorn

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    Biodiesel production from spent coffee grounds via ethanolic extraction and supercritical ethanol transesterification: technoeconomic analysis and life cycle assessment
    (2026-01-01)
    Supang, Wirasinee
    ;
    Ngamprasertsith, Somkiat
    ;
    Nimmanterdwong, Prathana
    ;
    Sakdasri, Winatta
    ;
    Hunsub, Panusorn
    Previous studies have developed biodiesel production methods from spent coffee grounds (SCGs) via ethanolic extraction and supercritical ethanol transesterification (EE-SET) to minimize the energy consumption associated with solvent evaporation. In this study, we perform a technoeconomic analysis (TEA) and a life cycle assessment (LCA) to evaluate the profitability and sustainability of EE-SET. The material and energy balances in the TEA and LCA were derived from Aspen Plus<sup>®</sup> V12 based on experimental data from previous research. The results from the base case indicated that EE-SET was unprofitable and posed a considerable environmental burden by disposing of defatted SCG (DSCG) as solid waste due to two main issues. First, the amount of DSCG was 4.8 times greater than the total biodiesel and glycerol produced. Second, landfilling DSCG incurred waste treatment costs and induced greenhouse gas (GHG) emissions simultaneously. Selling DSCG as a feedstock for solid fuel pellets offers an effective strategy for achieving profitability and sustainability. As such, EE-SET addresses the economic drawback of generating profit from selling DSCG while reducing waste treatment costs. Using DSCG as a solid fuel for environmental benefits increases ash disposal credit and substantially reduces GHG emissions. The incorporation of pelletizing units or biomass power plants into EE-SET represents a promising avenue for future research.
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    Life cycle assessment of spray-drying encapsulation of crude peptides produced from defective green coffee beans
    (2025-03-01)
    Hunsub, Panusorn
    ;
    Ngamprasertsith, Somkiat
    ;
    Prichapan, Nattapong
    ;
    Sakdasri, Winatta
    ;
    Karnchanatat, Aphichart
    The use of agricultural waste for the production of value-added ingredients was investigated. Defective green coffee beans were utilized as alternative feedstocks for the production of peptides. The crude peptides were produced by enzymatic hydrolysis, followed by membrane filtration and centrifugation. Subsequently, hydrolysate peptides were encapsulated via spray drying with maltodextrin as the carrier, facilitating their use as a functional ingredient in food supplements. This study aimed to produce hydrolysate peptides from defatted defective green coffee beans by supercritical carbon dioxide with ethanol as a cosolvent. Additionally, their environmental impacts (gate to gate) were evaluated and analyzed using the life cycle assessment approach. Data obtained from pilot-scale production of 10 L of oil extract and 50 L of peptide extract were used to examine the environmental consequences of the various treatments. Two scenarios of downstream processing were examined for equivalent antioxidant activity: spray-drying without maltodextrin (Scenario 1) and spray-drying with maltodextrin encapsulation (Scenario 2). Scenario 2 consumed less electricity than Scenario 1 due to its shorter operating time and lower environmental impact compared to Scenario 1. Additionally, its encapsulated form is suitable for antioxidant retention and the storage of functional ingredients. The greatest environmental impact arose from supercritical carbon dioxide with ethanol extraction, which affected 8 out of 15 midpoint categories, followed by spray dry encapsulation, which affected 7 out of 15 midpoint categories. Solutions for improvement, such as carbon dioxide and/or ethanol recycling, and alternative heat and electricity sources are also discussed.
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    Effects of Defatting Pretreatment on Polysaccharide Extraction from Rambutan Seeds Using Subcritical Water: Optimization Using the Desirability Approach
    (2024-07-01)
    Nilmat, Kamonthip
    ;
    Hunsub, Panusorn
    ;
    Ngamprasertsith, Somkiat
    ;
    Sakdasri, Winatta
    ;
    Karnchanatat, Aphichart
    Rambutan seeds are by-products generated from fruit-processing factories; the leftover seeds are buried in landfills, generating methane emissions. This work aimed to extract polysaccharides (POLS) from rambutan seeds by using subcritical water extraction (SWE). The effects of defatting pretreatment and operating parameters in SWE were investigated using a Box–Behnken design. The results show that defatting pretreatment significantly enriched the POLS yield, while it had no significant effect on the total sugar content. Using the desirability approach, the suitable feedstock for SWE was defatted rambutan seeds. The maximum desirability of 0.86 was found at a temperature range of 145–150 °C, an extraction time of 15 min, and a liquid–solid ratio of 10:1. The POLS yield and total sugar content were in the range of 52.33–55.63 g/100 g feedstock and 83.37–87.45 g/100 g POLS, respectively. The extracted POLS had an equivalent molecular weight of 413.70 kDa that could be used as an extender in plant-based products. In conclusion, the defatting pretreatment of rambutan seeds not only improved the POLS yield obtained via SWE but also generated additional lipids that could be utilized as an unconventional source of specialty fat.
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    Production of Coffee oil and Bioactive Peptides from Spent Coffee Grounds via Supercritical Carbon Dioxide Extraction and Enzymatic Hydrolysis
    (2024-04-01)
    Hunsub, Panusorn
    ;
    Ponmana, Kanokporn
    ;
    Ngamprasertsith, Somkiat
    ;
    Sakdasri, Winatta
    ;
    Karnchanatat, Aphichart
    Supercritical carbon dioxide (SCCO<inf>2</inf>) extraction was applied for recovering non-polar compounds in spent coffee grounds (SCGs) before enzymatic hydrolysis was performed. The SCCO<inf>2</inf> extracted oil yield of 11.93 wt% was observed at 30 MPa and 50 °C. The detectable volatile compounds in SCGs oil were aldehydes and flavor compounds classified as furans. Although the degree of hydrolysis of SCGs and defatted SCGs (DFSCGs) were not significantly different, the soluble protein of DFSCGs was higher than that of SCGs. Electrophoretic profiles of SCGs and DFSCGs comprise polypeptide bands at < 20 and ~ 24 kDa, which are mainly derived from 11S globulin subunits. After hydrolysis, the molecular masses of 4–20 and 24 kDa were virtually eliminated, thus releasing polypeptides with molecular masses < 4 kDa. Furthermore, the DFSCGs hydrolysate had higher total phenolic content and antioxidant capacity than that of SCGs hydrolysate. Pretreatment of SCGs with SCCO<inf>2</inf> enhances enzymatic accessibility, improves the quality of protein hydrolysate, and procures SCGs oil as a by-product. Graphical Abstract: (Figure presented.)