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Author: search.filters.author.Inpota, Prawpan

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    A Novel Cross Injection Analysis for Simultaneous Multi-Determination of Diabetic Nephropathy Biomarkers in Urine
    (2026-05-01)
    Inpota, Prawpan
    ;
    Choengchan, Nathawut
    This work presents, for the first time, a novel cross injection analysis (CIA) system for the simultaneous multi-determination of key biomarkers associated with diabetic nephropathy—namely, albumin, creatinine, and glucose—within a single analytical run. Unlike conventional flow-based techniques that rely on sequential measurements, the proposed CIA platform integrates multiple analytical pathways into a unified design, enabling one-shot multi-analyte analysis without the need for complex separation units or injection valves. The system employs peristaltic pumps and a rectangular platform with orthogonal flow channels, allowing concurrent aspiration and efficient transport of reaction products to compact detectors. Albumin determination was based on ion-association with tetrabromophenolphthalein ethyl ester. Creatinine was measured using the Jaffé reaction. Glucose was colorimetrically detected via its reaction with 3,5-dinitrosalicylic acid. The developed CIA provides enhanced sensitivity through its pre-mixing effect, enabling reliable quantification of trace analytes. Excellent analytical performance was achieved, including wide linear ranges (r<sup>2</sup> > 0.99), good precision (RSD < 7%), and rapid analysis (5 min). The method was validated against established reference methods, showing no significant differences, and successfully applied to urine with satisfactory recoveries (84.8–107.3%). Importantly, the proposed system adheres to green chemistry by minimizing reagent consumption and waste generation, offering a sustainable approach for multi-parameter clinical analysis.
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    Microfluidic analysis with front-face fluorometric detection for the determination of total inorganic iodine in drinking water
    (2018-01-01)
    Inpota, Prawpan
    ;
    Strzelak, Kamil
    ;
    Koncki, Robert
    ;
    Sripumkhai, Wisaroot
    ;
    Jeamsaksiri, Wutthinan
    A microfluidic method with front-face fluorometric detection was developed for the determination of total inorganic iodine in drinking water. A polydimethylsiloxane (PDMS) microfluidic device was employed in conjunction with the Sandell-Kolthoff reaction, in which iodide catalyzed the redox reaction between Ce(IV) and As(III). Direct alignment of an optical fiber attached to a spectrofluorometer was used as a convenient detector for remote front-face fluorometric detection. Trace inorganic iodine (IO <inf>3</inf> <sup>-</sup> and I <sup>-</sup> ) present naturally in drinking water was measured by on-line conversion of iodate to iodide for determination of total inorganic iodine. On-line conversion efficiency of iodate to iodide using the microfluidic device was investigated. Excellent conversion efficiency of 93 - 103% (%RSD = 1.6 - 11%) was obtained. Inorganic iodine concentrations in drinking water samples were measured, and the results obtained were in good agreement with those obtained by an ICP-MS method. Spiked sample recoveries were in the range of 86%(±5) - 128%(±8) (n = 12). Interference of various anions and cations were investigated with tolerance limit concentrations ranging from 10-6 to 2.5 M depending on the type of ions. The developed method is simple and convenient, and it is a green method for iodine analysis, as it greatly reduces the amount of toxic reagent consumed with reagent volumes in the microfluidic scale.