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Author: search.filters.author.Insung, A.Subject: search.filters.subject.Insecticidal activity

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    Effectiveness of bastard oleaster (Elaeagnus latifolia) extracts against the nymph of mealybug (Phenacoccus manihoti)
    (2019-01-01)
    Pumnuan, J.
    ;
    Sannongmueang, T.
    ;
    Inyod, T.
    ;
    Insung, A.
    Bastard oleaster (Elaeagnus latifolia) is sometimes gathered from subtropical wild for local use as food, medicine and fuel. The objective of this study was to investigate the insecticidal properties of extracts from leaves and fruits of bastard oleaster against the nymph of mealybug (Phenacoccus manihoti) by using leaf dipping method. The different extracts of bastard oleaster as water extract by decoction method, ethanol and hexane extracts by percolation and Soxhlet methods were applied with various concentrations; 0 (5% tween-20 water) 1, 2, 3, 4 and 5% (w/v), and imidacloprid insecticide used as positive control. Then the insect mortality was observed at 24 and 48 h, as well as the repellent index (%RI) was observed at 24 h. The results showed that ethanolic extract from bastard oleaster fruits by Soxhlet method and leaves by percolation method were highly effective in killing the nymph of mealybug with the LC50 at 48 h for 2.64 and 2.83%, respectively. The bastard oleaster leaves extracted by percolation and Soxhlet at 5% concentrations showed rather strong effect in repellence when the nymph of mealybug with >52% RI was found within 24 h. In addition, all extracts of bastard oleaster at 5% concentrations were more effective against the nymph of mealybug than imidacloprid insecticide at 24 h. Therefore, extracts from this wild plant, bastard oleaster leaves would be promoted and used as botanical insecticide in the future.
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    GC-MS analysis and biopesticide properties of different crude extracts of Annona squamosa and Annona muricata
    (2019-01-01)
    Deewatthanawong, R.
    ;
    Kongchinda, P.
    ;
    Deewatthanawong, P.
    ;
    Pumnuan, J.
    ;
    Insung, A.
    Most common compounds identified by GC-MS analysis of ethanolic extracts from leaves of Annona squamosa (sweetsop) and Annona muricata (soursop) were neophytadiene, hexadecanoic acid, and trans-caryophyllene. Hexadecenoic acid and ethyl oleate were identified in seeds of A. muricata, while only methyl-p-tert-butyl phenylacetate was identified in seeds extract of A. squamosa. Crude extracts were tested for their antifungal activities against postharvest plant pathogens, insecticidal activities against brown planthopper, Nilaparvata lugens (Stål) and cassava mealybug, Phenacoccus manihoti Matile-Ferrero. Results showed low inhibitory effect (less than 20%) for the pathogenic fungi insecticidal test against cassava mealybug of all crude brown planthopper treated with 5% seed extract of A. squamosa, indicating its potential as an alternative botanical insecticide. Further studies are required to improve yield and extract quality.