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    Film seeds coating with hexane extracts from Illicium verum Hook.f. and Syzygium aromaticum (L.) Merrill & Perry for controlling Callosobruchus maculatus (F.) and Callosobruchus chinensis L
    (2021-05-01)
    Pumnuan, Jarongsak
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    Sarapothong, Kritima
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    Sikhao, Potjana
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    Pattamadilok, Chutichot
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    Insung, Ammorn
    BACKGROUND: The application of film seed coating with some plant extracts against stored insect pests was an alternative approach for insect protection instead of using chemical insecticides. The overuse of insecticides has contributed to both human health and environmental problems. The hexane extracts of Illicium verum (HS) and Syzygium aromaticum (HC) were evaluated against bruchid beetles (Callosobruchus maculatus and Callosobruchus chinensis). The insecticidal activities and enhancing seed germination after being coated by various concentrations of extracts, purified fraction (PF) of extracts, standard chemicals of PF, fipronil insecticide (FI), and coating agent groups were evaluated after stored. RESULTS: Both HS and HC at 3% concentrations were more effective against C. maculatus than C. chinensis with LT<inf>50</inf> values of 1.60–1.90 and 3.44–3.62 h, respectively. They could also kill those insects with a higher number than that of standards. The HS and HC could inhibit the progeny population of those insects over 95.6% with no significant difference compared to the FI group. The eugenol standard was the most effective and completely inhibited the emergence compared with trans-anethol standard and PFs. The seed that was coated with 1% of HS and stored at 4 °C for 6 months showed no effect on seed germination with no significant difference between FI and control groups. CONCLUSION: The application of 1% of HS incorporated in the film seed coating against bruchid beetles and did not affect seed germination quality after 6 months. © 2021 Society of Chemical Industry.
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    Carbaryl insecticide decomposition after application on oyster and jew’s ear mushrooms cultivations
    (2021-04-01)
    Pumnuan, Jarongsak
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    Insung, Ammorn
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    Phutphat, Sudjai
    One of the major problems in the cultivation of oyster and Jew’ s ear mushrooms is the damage from various insect and mite pests. Farmers occasionally used pesticides to control the pests, resulting in contamination of the product. The purpose of this study was to evaluate the decomposition of carbaryl insecticide found in oyster and Jew’ s ear mushrooms. The carbaryl residue, in both mushrooms and on the associated growing bags, was then investigated. The carbaryl insecticide, at different concentrations (0, 850 (recommendation) and 1,700 ppm) was sprayed 2 times: 3 days before, and during the first cap opening. The samples were collected after final spraying on days 0, 1, 3 and 6. The samples were extracted and analyzed for carbaryl residues by GC-MS. The results showed that the oyster and Jew's ear mushrooms sprayed with the 850 ppm carbaryl had residue concentrations of less than 0. 45 ppm on the 3<sup>rd</sup> day. Additionally, the growing bags presented carbaryl residues on day 0 with 1 . 86 and 0. 13 ppm for oyster and Jew’ s ear mushrooms, respectively. The experiment of 1,700 ppm carbaryl treatment resulted in residues on the 6<sup>th</sup> day that were less than 0. 63 ppm. The contaminations observed in the oyster and Jew’ s ear growing bags on day 0 were 3. 75 and 0. 80 ppm, respectively. Our study would recommend that in cases where carbaryl insecticide use in mushroom cultures is imperative, carbaryl application at the recommendation dose should be made 3 days before the harvesting period, in the interest of consumers safety.
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    Acaricidal activity of essential oil nanoemulsion against the african red mite (Eutetranychus africanus)
    (2021-04-01)
    Doungnapa, Thanaporn
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    Pumnuan, Jarongsak
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    Insung, Ammorn
    The African red mite (Eutetranychus africanus [Tucker]) is an important pest species of some economic plants. The objective of the present study was to evaluate the effectiveness of nanoemulsions consisting of citronella grass, lemongrass, clove, and cinnamon essential oils and their main chemical compounds against the African red mite; their toxicity, egg-laying inhibition, and repellent effect on the mite were determined. Essential oil nanoemulsions (nEOs) were prepared by mixing the essential oils with different surfactants and co-surfactants. Approximately 10 to 15 female mites were transferred to treated leaves placed on soaked cotton and kept in a Petri dish. Mite mortality and number of eggs laid were checked after 24 h. As for the repellency test, the selected test consisted of dipping a half leaf into the nEOs, while the other half leaf was dipped in a control (water). The repellency rate was evaluated after 24 h. Results revealed that citronella grass nEOs exhibited a highly toxic effect on the mite and only a 0.6% concentration caused 100% mite mortality. Geraniol, one of the main chemical compounds of citronella grass, represented the highest egg-laying inhibition at a 0.2% concentration with a mean of 0.1 egg compared with 4.7 eggs in the control. Furthermore, nEOs from citronella grass at a 0.1% concentration were extremely repellent against the mite with 95% repellency after 24 h. Therefore, the citronella grass essential oil nanoemulsion is appropriate for use to effectively control the African red mite.
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    The use of ozone for controlling european house dust mite, dermatophagoides pteronyssinus (Trouessart)
    (2020-09-01)
    Pumnuan, Jarongsak
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    Insung, Ammorn
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    Wangapai, Teerapong
    Hypersensitivity allergies are mostly caused by house dust mite (HDM), Dermatophagoides pteronyssinus (Trouessart). Common control methods for HDM include disposal of bedding sets and fabrics, vacuuming, washing, using plant extracts and applying chemicals. Hence, the application of ozone is a new alternative way to control HDM. The objectives of this experiment were to determine the efficacy of ozone as an HDM killer and to evaluate the allergic levels that remained after the application of ozone. The fumigation method was performed in a laboratory using ozone at the concentrations of 20, 30, 40 and 50 mg l-1 in glass chambers (1 m3) at 1, 2 and 3 h fumigation intervals. The mortality percentages of HDM were observed at 24 h after the treatment. The allergic levels appearing in supernatants were analyzed by an enzyme-linked immunosorbent assay (ELISA) method. Ozone fumigations of 30 mg l-1 concentration for 3 h completely killed HDM. In addition, ozone fumigations of ≥ 40 mg l-1 for 3 h reduced the amount of allergen by >50%, which was a significantly higher reduction than seen at 20-30 mg l-1 (35.8-45.8%). The study suggests the ozone fumigation at 30 mg l-1 concentration treated for at least 3 h, 40 mg l-1 for 2 h, or 50 mg l-1 for 1 h could be used as a new alternative method to control HDM.
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    Effects of seven plant essential oils on mortalities of chicken lice (Lipeurus caponis l.) adult
    (2020-01-01)
    Pumnuan, Jarongsak
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    Insung, Ammorn
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    Klompanya, Ampon
    Chicken lice (Lipeurus caponis L.) are external parasite of chicken and farmers often use malathion and carbaryl insecticides to control this parasite. However, the use of chemical insecticides may result in direct toxicity to chicken or contamination in chicken meats. Thus, this study investigated the effect of seven plant essential oils (EOs) against adult chicken lice. Contact bioassay was performed by laying 10 adult chicken lice on treated filter paper added with EOs at concentrations of 0 (95% ethanol as a control), 0.079, 0.157, 0.236 and 0.314 μl·cm<sup>-2</sup>. Mortality percentages were observed at 3, 6 and 12 h intervals after the treatments. Clove, cinnamon, turmeric and star anise EOs were able to eliminate the lice completely (100% mortalities) at 12 h exposure which were higher than lemon grass, citronella grass and piper EOs. Clove EO was the most effective oil with LC<inf>50</inf> of 0.132, 0.085 and 0.039 μl·cm<sup>-2</sup> at 3, 6 and 12 h, respectively. Furthermore, clove EO at the 0.157 μl·cm<sup>-2</sup> showed the highest mortalities with LT<inf>50</inf> at 1.438 h. Our study suggests that clove EO could be used as an alternative medicinal insecticide to control chicken lice in farms.
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    Insecticidal activity of essential oil formulas and their physiological effects on eggplant
    (2017-05-01)
    Pumnuan, Jarongsak
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    Khurnpoon, Lampan
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    Insung, Ammorn
    This study examined fumigation toxicity of 18 medicinal plant essential oils (EOs) against adult of aphid (Aphid gossypii) and whitefly (Bemisia tabaci). Then, non-target effects of the EO mixtures on physiological changes of eggplant (Solanum melongena) were tested. The insecticidal property and physiological toxicity of the fumigation formulas were also examined and compared to methyl bromide (MB) fumigation. The results showed that the eggplant fumigated with clove (Syzygium aromaticum) and lemon grass (Cymbopogon citratus) EOs mixture at the ratio of 1:3 (Cl1Le3) showed no significant physiological changes when compared to the control treatment. The formula resulted in similarly high mortalities (82-100%) of both insects when compared to MB. However, MB fumigation caused complete senescence appeared before day 3 observations. On the contrary, the eggplant fumigated with Cl1Le3 at 3 μL/L air showed no differences in the physiological changes when compared to the control throughout the 9-day examinations.
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    Fumigant toxicity of plant essential oils in controlling thrips, Frankliniella schultzei (Thysanoptera: Thripidae) and mealybug, Pseudococcus jackbeardsleyi (Hemiptera: Pseudococcidae)
    (2016-01-01)
    Pumnuan, Jarongsak
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    Insung, Ammorn
    Insecticidal activity of 18 plant essential oils against adults of thrips (Frankliniella schultzei (Trybom)) and larvae of mealybug (Pseudococcus jackbeardsleyi Gimpel & Miller) were primarily investigated by using fumigation method. Later, various concentrations of the highly effective essential oils (resulting in more than 80% mortality) were tested. Major constituents in the commonly effective essential oils against both insects were analyzed. Then, fumigant toxicities of the standard chemicals and the selected essential oils were studied and compared. The results showed that the essential oils of clove (Syzygium aromaticum Merr. & L.M. Perry), cinnamon (Cinnamomum bejolghota (Buch.-Ham.) Sweet) and lemon grass (Cymbopogon citratus (Dc.ex. Nees)) showed commonly high fumigant toxicity against the thrips and mealybug with the LC<inf>50</inf> at 1.14-1.48 and 1.12-1.58 μl/L air, respectively. The major chemicals found were eugenol in clove and cinnamon oils (97.100 and 82.054%, respectively) and citral (trans-citral and cis-citral) in lemongrass (69.730%). In general, the essential oil presented higher fumigant toxicity than the standard chemical. Study revealed that a combination of various compounds resulted in synergism and higher insecticidal activity.
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    Effects of insecticidal essential oil fumigations on physiological changes in cut Dendrobium sonia orchid flower
    (2015-09-01)
    Pumnuan, Jarongsak
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    Khurnpoon, Lampan
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    Insung, Ammorn
    This study investigated essential oil (EO) formulas with high insecticidal properties, but low physiological impacts on cut Dendrobium Sonia orchid flower. Fumigation toxicities of EOs from 18 medicinal plants at 2.0 and 3.0 μl/L air were examined against adults of thrips (Frankliniella schultzei) and larvae of mealybug (Pseudococcus jackbeardsleyi). The effective EO mixtures, optimal concentrations fumigation and air circulation periods were investigated. Then, field experiments were conducted, and changes in L*, a* and b* values, percentages of weight loss and anthocyanin contents of the EO-fumigated flower were observed and compared to the methyl bromide and control fumigations. The results showed that clove and cinnamon demonstrated high insecticidal properties against the insects (>85% mortality) and low physiological changes in the flower. In particular, fumigations with 2.0 μl/L air of a mixture between clove and cinnamon EOs (1:3) for 3 hr with 15- min air circulation demonstrated the highest thrips and mealybug mortalities (92.2 and 74.6%, respectively). The EO fumigation formula presented less impact on color change and anthocyanin content than methyl bromide fumigation which showed higher reduction of anthocyanin content (22.9 mg/100g FW) when compared to the control (13.6 mg/100g FW). The percentages of weight loss in the flower fumigated with EO, control and methyl bromide were about 10.4, 7.9 and 14.8%, respectively. In general, applications of EO at higher concentrations resulted in higher insect mortalities and more impacts on physiological changes which involved anthocyanin degradation and higher percentages of weight loss. Further studies might consider applications of clove and cinnamon EO formulas via other methods. In addition, revisions of the EO mixture can also be examined in order to obtain the most effective and environment friendly insect management approach.
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    Investigation of tannin crude extract from cassava leaves for mealy bug control
    (2013-12-01)
    Arirob, Wallop
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    Insung, Ammorn
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    Pumnuan, Jarongsak
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    Won-In, Krit
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    Dararutana, Pisutti
    Different concentrations of tannin crude extract from cassava leaves were used for direct spraying, dipping, choice and no-choice repellent test for controlling cassava mealy bug (Pseudococcus jackbeardsleyi Gimpel and Miller). By direct spray method, it was showed that tannin crude extract could not kill cassava mealy bug as insecticide but remarkably repelled mealy bug. Dipping method at 1,500 mg/l tannin concentration could only kill the mealy bug of 7.4% and seemed to decrease plant damage of 32.52% when compared with control. Field experiment revealed that spraying with 1,000 mg/l tannin extract concentration could keep cassava clean, less damaging of mealy bug as well as cassava could promote new apical bud, axially growth and straight trunk. It was wondering that extract from cassava leaves could be used as cassava mealy bug repellant. © 2013 American Scientific Publishers.
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    Acaricidal activities of plant essential oils from three plants on the mushroom mite, Luciaphorus perniciosus Rack (Acari: Pygmephoridae)
    (2010-04-26)
    Pumnuan, Jarongsak
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    Chandrapatya, Angsumarn
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    Insung, Ammorn
    Essential oils of Litsea cubeba, Litsea salicifolia and Melaleuca cajuputi were tested against Luciaphorus perniciosus by contact and fumigation methods. Contact toxicity bioassay was made by dropping essential oils at rates of 0, 0.06, 0.33, 0.66, 3.3, 6.6, 33, 66 and 99 μl/cm<sup>2</sup> into special mite cages. Fumigation was done in a knockdown chamber of 2.5x10<sup>4</sup> cm <sup>3</sup> in size at the concentrations of 0, 0.0012, 0.006, 0.012, 0.06, 0.12,0.6 and 1.2 μg/cm<sup>3</sup>. The essential oil of L. cubeba was found to be the most toxic to L. perniciosus by both contact and fumigation methods with LD<inf>50</inf> values equaling to 0.932 and 0.166 μg/cm<sup>3</sup>, respectively, followed by essential oil of L. salicifolia whose LD<inf>50</inf> values of 2.793 and 0.410 μg/cm<sup>3</sup> were recorded. Essential oil of M. cajuputi demonstrated a low effect on L. perniciosus as compared to those of L. cubeba and L. salicifolia. Essential oil formulations were delivered by using citronella grass or black pepper oils as major components and L. cubeba as minor component. Mite mortality rate increased when 0.01% L. cubeba oil was added to either citronella grass or black pepper oil at all treatments by contact method. Copyright 2010 Zoological Society of Pakistan.