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    Effects of seven plant essential oils on mortalities of chicken lice (Lipeurus caponis l.) adult
    (2020-01-01)
    Pumnuan, Jarongsak
    ;
    Insung, Ammorn
    ;
    Klompanya, Ampon
    Chicken lice (Lipeurus caponis L.) are external parasite of chicken and farmers often use malathion and carbaryl insecticides to control this parasite. However, the use of chemical insecticides may result in direct toxicity to chicken or contamination in chicken meats. Thus, this study investigated the effect of seven plant essential oils (EOs) against adult chicken lice. Contact bioassay was performed by laying 10 adult chicken lice on treated filter paper added with EOs at concentrations of 0 (95% ethanol as a control), 0.079, 0.157, 0.236 and 0.314 μl·cm<sup>-2</sup>. Mortality percentages were observed at 3, 6 and 12 h intervals after the treatments. Clove, cinnamon, turmeric and star anise EOs were able to eliminate the lice completely (100% mortalities) at 12 h exposure which were higher than lemon grass, citronella grass and piper EOs. Clove EO was the most effective oil with LC<inf>50</inf> of 0.132, 0.085 and 0.039 μl·cm<sup>-2</sup> at 3, 6 and 12 h, respectively. Furthermore, clove EO at the 0.157 μl·cm<sup>-2</sup> showed the highest mortalities with LT<inf>50</inf> at 1.438 h. Our study suggests that clove EO could be used as an alternative medicinal insecticide to control chicken lice in farms.
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    Insecticidal activity of essential oil formulas and their physiological effects on eggplant
    (2017-05-01)
    Pumnuan, Jarongsak
    ;
    Khurnpoon, Lampan
    ;
    Insung, Ammorn
    This study examined fumigation toxicity of 18 medicinal plant essential oils (EOs) against adult of aphid (Aphid gossypii) and whitefly (Bemisia tabaci). Then, non-target effects of the EO mixtures on physiological changes of eggplant (Solanum melongena) were tested. The insecticidal property and physiological toxicity of the fumigation formulas were also examined and compared to methyl bromide (MB) fumigation. The results showed that the eggplant fumigated with clove (Syzygium aromaticum) and lemon grass (Cymbopogon citratus) EOs mixture at the ratio of 1:3 (Cl1Le3) showed no significant physiological changes when compared to the control treatment. The formula resulted in similarly high mortalities (82-100%) of both insects when compared to MB. However, MB fumigation caused complete senescence appeared before day 3 observations. On the contrary, the eggplant fumigated with Cl1Le3 at 3 μL/L air showed no differences in the physiological changes when compared to the control throughout the 9-day examinations.
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    Item type:Publication,
    Fumigant toxicity of plant essential oils in controlling thrips, Frankliniella schultzei (Thysanoptera: Thripidae) and mealybug, Pseudococcus jackbeardsleyi (Hemiptera: Pseudococcidae)
    (2016-01-01)
    Pumnuan, Jarongsak
    ;
    Insung, Ammorn
    Insecticidal activity of 18 plant essential oils against adults of thrips (Frankliniella schultzei (Trybom)) and larvae of mealybug (Pseudococcus jackbeardsleyi Gimpel & Miller) were primarily investigated by using fumigation method. Later, various concentrations of the highly effective essential oils (resulting in more than 80% mortality) were tested. Major constituents in the commonly effective essential oils against both insects were analyzed. Then, fumigant toxicities of the standard chemicals and the selected essential oils were studied and compared. The results showed that the essential oils of clove (Syzygium aromaticum Merr. & L.M. Perry), cinnamon (Cinnamomum bejolghota (Buch.-Ham.) Sweet) and lemon grass (Cymbopogon citratus (Dc.ex. Nees)) showed commonly high fumigant toxicity against the thrips and mealybug with the LC<inf>50</inf> at 1.14-1.48 and 1.12-1.58 μl/L air, respectively. The major chemicals found were eugenol in clove and cinnamon oils (97.100 and 82.054%, respectively) and citral (trans-citral and cis-citral) in lemongrass (69.730%). In general, the essential oil presented higher fumigant toxicity than the standard chemical. Study revealed that a combination of various compounds resulted in synergism and higher insecticidal activity.