KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/kmitl/1

Browse

Settings

Author: search.filters.author.Intaraudom, ChakapongSubject: search.filters.subject.Endophytic actinobacterium

Search Results

Now showing 1 - 2 of 2
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Streptomyces endocoffeicus sp. nov., an endophytic actinomycete isolated from Coffea arabica (L.)
    (2021-11-01)
    Nammali, Achararak
    ;
    Intaraudom, Chakapong
    ;
    Pittayakhajonwut, Pattama
    ;
    Suriyachadkun, Chanwit
    ;
    Tadtong, Sarin
    An aerobic, non-motile, Gram-stain positive actinomycete, designated strain CA3R110<sup>T</sup>, was isolated from the surface-sterilised root of Coffea arabica L. collected from Lampang Province, Thailand. 16S rRNA gene sequence analysis indicated that strain CA3R110<sup>T</sup> was a member of the genus Streptomyces and showed the closest similarities to Streptomyces buecherae AC541<sup>T</sup> (99.2%), followed by Streptomyces rapamycinicus NRRL B-5491<sup>T</sup> (99.1%), Streptomyces luteoverticillatus NBRC 3840<sup>T</sup> (99.1%), Streptomyces coerulescens NBRC 12758<sup>T</sup> (99.1%), and Streptomyces iranensis HM 35<sup>T</sup> (99.0%). Strain CA3R110<sup>T</sup> contained LL-diaminopimelic acid in cell peptidoglycan, MK-9(H<inf>6</inf>), and MK-9(H<inf>8</inf>) as major menaquinone, iso-C<inf>16:0,</inf>iso-C<inf>15:0,</inf> C<inf>16:0</inf> as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside were detected in the cell. The chemotaxonomic characteristics possessed the typical properties of the genus Streptomyces. A low digital DNA–DNA hybridization (< 55.7%) and average nucleotide identity-blast (ANIb) (< 92.2%) values revealed that strain CA3R110<sup>T</sup> could be distinguished from any known Streptomyces species. With the differences in phenotypic and genotypic data, strain CA3R110<sup>T</sup> represents a novel species of genus Streptomyces, for which the name Streptomyces endocoffeicus sp. nov. is proposed. The type strain is CA3R110<sup>T</sup> (= TBRC 11245<sup>T</sup> = NBRC 114296<sup>T</sup>).
      2
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Streptomyces coffeae sp. Nov., an endophytic actinomycete isolated from the root of coffea arabica (l.)
    (2021-01-01)
    Nammali, Achararak
    ;
    Intaraudom, Chakapong
    ;
    Pittayakhajonwut, Pattama
    ;
    Suriyachadkun, Chanwit
    ;
    Tadtong, Sarin
    An endophytic actinobacterium, designated strain CA1R205<sup>T</sup>, was isolated from the surface-sterilized root of Coffea arabica L. collected from Ratchaburi province, Thailand. The taxonomic position of this strain was evaluated using a polyphasic approach. The strain produced light yellowish brown to dark brownish black substrate mycelium and greyish white aerial mycelium. The spiral spore chains were produced directly on aerial mycelium. CA1R205<sup>T</sup> was found to have ll-diaminopimelic acid in the cell peptidoglycan, galactose, glucose, mannose and ribose as whole-cell reducing sugars, MK-10(H<inf>4</inf>), MK-9(H<inf>6</inf>), MK-10(H<inf>2</inf>), MK-9(H<inf>4</inf>), MK-10(H<inf>6</inf>) and MK-10(H<inf>8</inf>) as menaquinones and iso-C<inf>15:0</inf>, anteiso-C<inf>15:0</inf>, iso-C<inf>16:0</inf> and C<inf>16:0</inf> as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were detected in the cells. These characteristics were consistent the typical chemotaxonomic properties of members the genus Streptomyces. The taxonomic affiliation at the genus level of this strain could be confirmed using its 16S rRNA gene sequence data. CA1R205<sup>T</sup> showed the highest 16S rRNA gene sequence similarity value to Streptomyces rapamycinicus NRRL B-5491<sup>T</sup> (98.9%), followed by Streptomyces iranensis HM 35<sup>T</sup> (98.8%). Digital DNA–DNA hybridization and average nucleotide identity-by blast (ANIb) values between CA1R205<sup>T</sup> and S. rapamycinicus NRRL B-5491<sup>T</sup> were 27.2 and 81.5%, respectively. The DNA G+C content of genomic DNA was 70.7 mol%. Due to the differences in physiological, biochemical and genotypic data, CA1R205<sup>T</sup> could be discriminated from its closest neighbour. Thus, CA1R205<sup>T</sup> should be recognized as representing a novel species of the genus Streptomyces, for which the name Streptomyces coffeae sp. nov. is proposed. The type strain is CA1R205<sup>T</sup> (=TBRC 11244<sup>T</sup>=NBRC 114295<sup>T</sup>).