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Author: search.filters.author.Intaraudom, ChakapongSubject: search.filters.subject.actinomycete

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    Gordonia aquimaris sp. nov., a novel marine actinobacterium isolated from seawater in the upper gulf of Thailand
    (2023-03-01)
    Pansomsuay, Rawirat
    ;
    Duangupama, Thitikorn
    ;
    Pittayakhajonwut, Pattama
    ;
    Intaraudom, Chakapong
    ;
    Suriyachadkun, Chanwit
    An actinobacterium strain, SW21<sup>T</sup>, was isolated from seawater collected in the upper Gulf of Thailand. Cells were Gram-stain-positive, aerobic and rod-shaped. Growth was observed from 15 to 37 °C and at pH 6–8. Maximum NaCl for growth was 14% (w/v). meso-Diaminopimelic acid, arabinose, galactose, glucose, rhamnose and ribose were detected in the whole-cell hydro-lysate. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside were detected as the phospholipids in the cells. The major menaquinones were MK-9(H<inf>2</inf> ) and MK-7(H<inf>2</inf> ). The major cellular fatty acids were C<inf>16:0</inf>, C<inf>18:1</inf> ω9c, C<inf>18:0</inf> and C<inf>18:0</inf> 10-methyl (TBSA). The 16S rRNA gene sequence data supported the assignment of strain SW21<sup>T</sup> to the genus Gordonia and showed that Gordonia mangrovi KCTC 49383<sup>T</sup> (98.7%) was the closest relative. Moreover, the average nucleotide identity-blast (85.5%) and digital DNA–DNA hybridization (30.7%) values between strain SW21<sup>T</sup> and its closest neighbour were below the threshold values for delineation of a novel species. The combination of genotypic and phenotypic data indicated that strain SW21<sup>T</sup> is representative of novel species of the genus Gordonia. The name Gordonia aquimaris sp. nov. is proposed for strain SW21<sup>T</sup>. The type strain is SW21<sup>T</sup> (=TBRC 15691<sup>T</sup>=NBRC 115558<sup>T</sup>).
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    Streptomyces sennicomposti sp. nov., an actinomycete isolated from compost of Senna siamea (Lam.)
    (2022-01-01)
    Duangupama, Thitikorn
    ;
    Pittayakhajonwut, Pattama
    ;
    Intaraudom, Chakapong
    ;
    Suriyachadkun, Chanwit
    ;
    Sirirote, Pramote
    A member of the genus Streptomyces, designated RCPT1-4<sup>T</sup>, was isolated from compost of Senna siamea (Lam.), collected from an agricultural area in Rayong province, Thailand. The spore morphology and the presence of ll-diaminopimelic acid in the peptidoglycan indicate that RCPT1-4<sup>T</sup> shows the typical properties of members of the genus Streptomyces. On the basis of the results of 16S rRNA gene sequence analysis, the strain should be classified as representing a member of the genus Streptomy-ces and was most closely related to Streptomyces fumigatiscleroticus NBRC 12999<sup>T</sup> with the highest 16S rRNA gene sequence similarity of 99.2%, followed by Streptomyces spiralis NBRC 14215<sup>T</sup> (99.0%). In addition, RCPT1-4<sup>T</sup> shared the highest average nucleotide identity by blast (ANIb) (86.0%), and digital DNA–DNA hybridization (dDDH) (32.1%) values with S. spiralis NBRC 14215<sup>T</sup>. Furthermore, several physiological and biochemical differences were observed between RCPT1-4<sup>T</sup> and the closely related type strains of species with validly published names. These taxonomic data indicated that RCPT1-4<sup>T</sup> could be considered to represent a novel species of the genus Streptomyces and the name Streptomyces sennicomposti sp. nov. is proposed for this strain. The type strain is RCPT1-4<sup>T</sup> (=TBRC 11260<sup>T</sup>=NBRC 114303<sup>T</sup>).
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    Streptomyces acidipaludis sp. nov., an actinobacterium isolated from peat swamp forest soil
    (2022-01-01)
    Nammali, Achararak
    ;
    Duangupama, Thitikorn
    ;
    Pittayakhajonwut, Pattama
    ;
    Intaraudom, Chakapong
    ;
    Suriyachadkun, Chanwit
    An actinobacterium strain PLK6-54<sup>T</sup> was isolated from Lankwai peat swamp forest soil collected from Yala province, Thailand. Strain PLK6-54<sup>T</sup> exhibited morphological and chemotaxonomic properties described for the genus Streptomyces. It formed a spiral spore chain directly on aerial mycelium. Growth was observed between 20 and 40 °C and at pH 5–8. The maximum NaCl for growth was 2% (w/v). ll-Diaminopimelic acid, arabinose and ribose were detected in the whole-cell hydrolysate. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside were detected as the phospholipids. The major menaquinones were MK-10(H<inf>2</inf>) and MK-9(H<inf>6</inf>). The major cellular fatty acids were iso-C<inf>16:0</inf>, anteiso-C<inf>15:0</inf> and iso-C<inf>14:0</inf>. 16S rRNA gene sequence data supported the assignment of strain PLK6-54<sup>T</sup> to the genus Streptomyces and showed that Streptomyces rubidus NBRC 102073<sup>T</sup> (99.0%) was the closest relative. Moreover, the average nucleotide identity-blast (85.5%) and digital DNA–DNA hybridization (30.7%) values reported between strain PLK6-54<sup>T</sup> and its closest neighbour were below the threshold values for delineation of a novel species. Strain PLK6-54<sup>T</sup> could be distinguished from related validly described Streptomyces species by several phenotypic properties. The combination of genotypic and phenotypic data indicated that strain PLK6-54<sup>T</sup> is representative of a novel species of the genus Streptomyces. The name Streptomyces acidipaludis sp. nov. is proposed for strain PLK6-54<sup>T</sup>. The type strain is PLK6-54<sup>T</sup> (=TBRC 11250<sup>T</sup>=NBRC 114297<sup>T</sup>).