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Author: search.filters.author.Janyasupab, MetiniSubject: search.filters.subject.Biosensor

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    Study of ni2+/ni3+ redox couple and electrocatalytic responses of ni on nitrogen-doped carbon for urea non-enzymatic detection
    (2021-01-01)
    Asavakijthananont, Narawee
    ;
    Janyasupab, Metini
    A novel redox couple of metallic nickel (Ni) catalyst can become a great candidate of non-enzymatic detection. By taking advantage of fast electron transfer, Ni redox couples can be tailored as pseudo-enzyme in urea measurement. In this study, Ni catalyst on nitrogen doped carbon (Ni-NC) was synthesized and characterized morphological, elemental, and electrocatalytic properties in comparison to different configuration of pure nickel (Ni), Ni with carbon (Ni-C), and bare carbon electrode, assessed by cyclic voltammetry and differential pulse voltammetry. By examining various Ni redox couples in rapid electron transfer process, the prominent anodic and cathodic peaks of Ni<sup>2+</sup>/Ni<sup>3+</sup> were applicable to detect urea in the detection range of 1-20 mM, with an excellent sensitivity and relative standard deviation of 1.634 µA.mM<sup>-1</sup> (R<sup>2</sup> of 0.989) and 4.89%, respectively. Therefore, Ni-NC can find practical applications for material sensing device toward non-enzymatic urea measurement.
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    Development of wireless based potentiostat in biomedical applications
    (2019-03-01)
    Janyasupab, Metini
    ;
    Asavakijthananont, Narawee
    Wirelessly integrated and portable electronics designs of analytical systems are of great importance to support future technological platforms including internet of things (IoT), artificial intelligence (AI), precision medicine, and wearable chemical/biological sensing systems. In this study, a simple and robust prototype of the so-called potentiostat, electrochemical instrumental system, with a wireless data acquisition and user control is demonstrated. With a wide range of potential applications, the developed system can be conveniently customizable to detect chemical of interest in food safety, continuous at-home diagnosis, environmental monitoring, and low-power energy storage. The device is comprised of two major units: a modified front-end sensor (Texas Instrument, LMP91000EVM), and a development board (Espressif, ESP32) implementing with a programmable saw tooth waveform to assess cyclic voltammetry (CV), the most common electrochemical measurement with the three-electrode configuration, namely working electrode, reference electrode and auxiliary electrode. In particular, the prototype shows a good agreement of CV in a standard electron transfer experiment by monitoring the interchange state of Fe<sup>2+</sup> and Fe<sup>3+</sup> ion in ferricyanide solution with potassium chloride supported, in comparison to that of the commercial potentiostat. Furthermore, the effects of different scan rate (0.05 to 0.15 V/s) and different ferricyanide concentrations from 1 to 7.5 millimolar are also evaluated to quantify sensing performance. Based on the obtained results, the calibration curve of anodic peak and cathodic peak current are linearly correlated with R<sup>2</sup> of 0.9987 and 0.9990, respectively, providing a cost-effective (less than USD 80) and portable solution to the future integration of sensing technology.
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    Electrocatalytic study of low-cost bimetallic cobalt/iron catalyst on carbon for non-enzymatic glucose sensor in human urine
    (2019-01-01)
    Janyasupab, Metini
    ;
    Liu, Chen Wei
    This study investigated an electrocatalytic behavior of non-enzymatic glucose detection in urine by using low-cost non-precious metal CoFe catalyst on carbon (C) supported. The bimetal catalyst was prepared by the reduction of oleic acid and loaded 10% wt. metal onto the activated carbon. Due to the synergistic effect, CoFe exhibited its intrinsic electrocatalytic property, suitable for the chemisorption of glucose molecule and the d-electron of metal. For morphology and elemental composition, CoFe/C was characterized by Transmission Electron Microscopy (TEM), and X-ray Energy Dispersive Spectroscopy (EDS) and X-ray Diffraction (XRD), exhibiting Co(111) and Fe2O3(104) with the nanocluster average diameter of 25 nm. More importantly, electrocatalytic behaviors of CoFe/C were assessed by cyclic voltammetry (CV) and Differential Pulse Voltammetry (DPV) on the glassy carbon rotating disk electrode for glucose detection (0-3 mM) in modified artificial urine (mAUM), and human urine specimens. In particular, excellent sensitivities from the lower range of glucose level (< 1 mM) and the higher level by DPV in mAUM were estimated to be 318.42 and 82.20 µA.cm<sup>-2</sup>.mM<sup>-1</sup> with the correlation coefficient (R<sup>2</sup> ) values of 0.90 and 0.94, respectively. Furthermore, the as-prepared CoFe/C biosensor also demonstrated practical measurement in human urine sample with the sensitivity of 59.72 µA.cm<sup>-2</sup>.mM<sup>-1</sup> (R<sup>2</sup> = 0.99) without any electron facilitators (e.g. sodium hydroxide), thereby providing a promising cost-effective catalyst design for future technology of non-enzymatic glucose sensing applications in urine.