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Author: search.filters.author.Ji, Dongqing

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    RhIr@MoS2 nanohybrids based disposable microsensor for the point-of-care testing of NADH in real human serum
    (2020-08-01)
    Ji, Dongqing
    ;
    Ying, Zi
    ;
    Zhang, Yuan
    ;
    Chen, Wei
    ;
    Janyasupab, Metini
    Dihydronicotinamide adenine dinucleotide (NADH) is an important enzyme in all living cells, which is found to be abnormally expressed in cancer cells. Since it is redox-active, an electrochemical detection method would be suitable for monitoring its concentration in biological fluids. Here we present a strategy for specific determination of NADH in real human serum by using RhIr@MoS<inf>2</inf> nanohybrids based microsensor. To implement the protocol, RhIr nanocrysrals are in-situ grown onto MoS<inf>2</inf> interlayers forming a nanohybrid structure (RhIr@MoS<inf>2</inf>). After being locally deposited on an electrochemical microsensor, it could be used for the analysis of NADH. The developed RhIr@MoS<inf>2</inf> nanohybrids based microsensor possesses the ability for analyzing NADH at the applied potential of 0.07 V (much lower than most reported values). The detection limit is evaluated as low as 1 nmol/L even in bovine serum albumin (BSA) media. In addition, the sampling analysis of human serum from cancer patients and health controls shows that the microsensor displays good diagnostic sensitivity and specificity, illustrating that this developed detection technique is a relatively accurate method for measuring NADH in biological fluids. The proposed electrochemical microsensor assay also owns the benefits of convenience, disposable and easy processing, which make it a great possibility for future point-of-care cancer diagnosis.
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    Phase-Regulated Sensing Mechanism of MoS2 Based Nanohybrids toward Point-of-Care Prostate Cancer Diagnosis
    (2020-05-01)
    Ying, Zi
    ;
    Feng, Lingyan
    ;
    Ji, Dongqing
    ;
    Zhang, Yuan
    ;
    Chen, Wei
    Alpha-methylacyl-CoA racemase (AMACR) has been proven to be consistently overexpressed in prostate cancer epitheliums, and is expected to act as a positive biomarker for the diagnosis of prostate carcinoma in clinical practice. Here, a strategy for specific determination of AMACR in real human serum by using an electrochemical microsensor system is presented. In order to implement the protocol, a self-organized nanohybrid consisting of metal nanopillars in a 2D MoS<inf>2</inf> matrix is developed as material for the sensing interface. The testing signal outputs are strongly enhanced with the presence of the nanohybrids owing to that the metal pillars provide an efficient mass difussion and electron transfer path to the MoS<inf>2</inf> film surface. Furthermore, the phase-regulated sensing mechanism over MoS<inf>2</inf> is noticed and demonstrated by density functional theory calculation and experiments. The explored MoS<inf>2</inf> based nanohybrids are employed for the fabrication of an electrochemical microsensor, presenting good linear relationship in both ng µL<sup>−1</sup> and pg µL<sup>−1</sup> ranges for AMACR quantification. The sampling analysis of human serum indicates that this microsensor has good diagnostic specificity and sensitivity toward AMACR. The proposed electrochemical microsensor system also demonstrates the advantages of convenience, cost-effectiveness, and disposability, resulting in a potential integrated microsystem for point-of-care prostate cancer diagnosis.