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Author: search.filters.author.Kanchanadumkerng, Pimpikar

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    Anti-oral streptococci and anti-biofilm properties of Etlingera pavieana essential oil and its bioactive compounds proposed for an alternative herbal mouthwash
    (2024-05-30)
    Wongsariya, Karn
    ;
    Lapirattanakul, Jinthana
    ;
    Chewchinda, Savita
    ;
    Kanchanadumkerng, Pimpikar
    Oral streptococci are the major group of bacteria in the oral cavity. Some of their species cause oral diseases that may lead to tooth loss and quality-of-life reduction, such as dental caries. One of prevention techniques to promote oral health is rinsing mouthwash after toothbrushing. This study aimed to determine the potential uses of local food, also remedy, plant in Thailand called Reaw-Horm or Etlingera pavieana for alternative herbal mouthwash. The essential oil from E. pavieana rhizome (Eo) is used for anti-streptococci including Streptococcus mutans and Streptococcus sobrinus and anti-biofilm activities. The main components of Eo are methyl chavicol (MC) and trans-anethole (TA). The disk diffusion method showed the inhibition zone of Eo in a dose-dependent manner. The minimum inhibitory concentration (MIC) of Eo and TA was >1.6 % v/v, and 0.4 % v/v of MC. Regarding anti-biofilm activities, MC showed nearly equal anti-biofilm formation of S. mutans and S. sobrinus, whereas Eo and TA acted toward S. sobrinus more than S. mutans biofilm. Sub-MIC killing effects on cells under biofilm were observed in Eo and MC. Therefore, MC was recommended as an active compound for anti-streptococci activities. Biocompatibility of Eo and MC were shown to be safe for epidermal cell lines. Herbal mouthwashes containing Eo were developed and had antioxidant and antimicrobial actions with established for 3 months. This study provides in vitro support on the use of herbal mouthwash with antioxidant and antimicrobial activities for dental caries prevention and well-being of individuals.
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    Effects of natural sugar on acidogenic potential, biofilm biomass, and antiseptic resistance of oral streptococci
    (2021-01-01)
    Kanchanadumkerng, Pimpikar
    ;
    Wongsariya, Karn
    Natural sugar is deliberated as ordinary, non-chemical, and healthy alternatives. However, a diet rich in sugar is well documented as a causative agent for dental caries. The purpose of this study was to investigate the effects of natural sugars, including raw cane, palmyra palm and coconut sugar on the acidogenic profiles, biofilm formation and antiseptic treatment efficacy compared with refined sugar. The study was based on single-species and dual-species of Streptococcus mutans ATCC 25175 and Streptococcus sobrinus ATCC 33402. Our results showed that sucrose was a major component of all samples, with percentages of relative content higher than 89.0. Palmyra sugar gave the least pH change at 180 min of 4.84-4.93, which indicated it was the least acidogenic. Coconut sugar formed the lowest level of biofilm biomass compared to refined sugar (p < 0.05) and other samples. Antiseptic treatment was performed to study the level of percent eradication of bacterial plaque using MTT assay to determine cell viability under biofilm in each sugar medium. Biofilm derived from coconut sugar had a susceptibility to antiseptic treatment with 56.2-61.99% eradication which was higher than the biofilm from palmyra and raw cane sugar. As a result, this study points out the effects of various natural sugars (especially different sources of plant material) on cariogenic potential. However, further experiment should be done to confirm the results in vivo and further study the cariogenic effects of diet supplementation of these fermentable sugars.
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    Influence of Gallic Acid and Thai Culinary Essential Oils on Antibacterial Activity of Nisin against Streptococcus mutans
    (2021-01-01)
    Jiamboonsri, Pimsumon
    ;
    Kanchanadumkerng, Pimpikar
    Streptococcus mutans is a well-known oral pathogen commonly associated with a normal dental problem and life-threatening infection. A bacteriocin nisin and the plant-derived compounds including gallic acid (GA) and Thai culinary essential oils (EOs) have been reported to have activity against oral pathogens. However, their synergistic interaction against S. mutans has not been explored. The purposes of this study were primarily to investigate anti-S. mutans properties and the antibiofilm formation of nisin, GA, and five EOs by using the broth microdilution method. Besides, the morphological change, killing rate, and antibacterial synergism were determined by scanning electron microscopy (SEM), time-kill assay, and checkerboard method, respectively. The results demonstrated that kaffir lime leaf (KLL) oil, lemongrass (LG) oil, and GA showed a potent anti-S. mutans activity and inhibited biofilm formation with the possible mechanism targeted on the cell membrane. Additionally, KLL oil revealed anti-S. mutans synergism with GA, LG oil, and chlorhexidine with the fractional inhibitory concentration (FIC) indexes ≤ 0.5. Interestingly, GA displayed a high potential to enhance anti-S. mutans activity of nisin by lowering the minimum inhibitory concentrations (MICs) to at least 8-fold in a bacteriostatic manner. These results suggest that GA and KLL oil may be potentially used as an adjunctive therapy along with nisin and chlorhexidine to control S. mutans infection.