KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/123456789/1

Browse

Search Results

Now showing 1 - 10 of 10

Some of the metrics are blocked by your
consent settings
Evaluation of bio-formulation from Chaetomium elatum ChE01 to control banana anthracnose caused by Colletotrichum musae
(2019-01-01)
Soytong, K.
;
Suthammarit, S.
;
Kanokmedhakul, S.
Anthracnose of banana is proved to cause by Colletotrichum musae. Chaetomium elatum ChE01 inhibited the growth of C. musae within 30 days in bi-culture plates over 60 % and inhibited the spore production of C. musae of 57 %. The metabolites from C. elatum ChE01 actively fungal activity against C. musae for spore inhibition which crude methanol, crude ethyl acetate and crude hexane extracts at the ED<inf>50</inf> value was 5.43, 7.43 and 19.62 ppm., respectively. The new bio-formulation of C. elatum ChE01 reduced disease incidence of banana anthracnose of 13.34 % compared to benomyl that reduced the disease incidence of 13.05 %. Moreover, the crude extract mixture of C. elatum ChE01 and spore suspension of C. elatum ChE01 reduced the anthracnose incidence of 2.13 and 2.04 %, respectively. The research finding is reported for the first time that bioformulations of C. elatum ChE01 could control banana anthracnose caused by C. musae.
Some of the metrics are blocked by your
consent settings
In vitro efficacy of Chaetomium brasiliense against Pythium spp. Causing root rot disease of tangerine
(2018-12-01)
Udompongsuk, M.
;
Soytong, K.
;
Kanokmedhakul, S.
;
Kanokmedhakul, K.
Root rot is one of the most serious disease in Tangerine which caused by Pythium spp. This study tested efficacy of Ch. brasiliense to control Pythium sp. by bi - culture, crude extracts and nano particles testing from Ch. brasiliense to control Pythium sp., in vitro. The results from the bi - culture testing, Ch. brasiliense inhibited mycelium growth and sporangia production by 42.50% and 48.41%, respectively. In crude extracts from Ch. brasiliense gave ED <inf>50</inf> values of 30.15, 58.71 and 37.25 ppm for the hexane, EtOAc and MeOH, respectively. The efficacy of nano particles against Pythium sp. with the ED <inf>50</inf> values of 2.69, 3.00 and 3.96 ppm for the hexane, EtOAc and MeOH, respectively.
Some of the metrics are blocked by your
consent settings
Nano-particles from Chaetomium brasiliense to control Phytophthora palmivora caused root rot disease in durian var Montong
(2018-12-01)
Tongon, R.
;
Soytong, K.
;
Kanokmedhakul, S.
;
Kanokmedhakul, K.
The antagonistic fungus, Chaetomium brasiliense was tested to control Phytophthora palmivora causing rot disease of Durian (Durio zibithenus L.) var Montong by crude extracts and Nano-particles derived from Ch. brasiliense. Crude extracts of antagonistic fungus was tested for antifungal biological activities. The crude extracts from antagonistic fungus with hexane, ethyl acetate and methanol were tested against P. palmivora. Crude ethyl acetate from Ch. brasiliense gave significantly against P. palmivora which the ED <inf>50</inf> values was 17.46 µg/ml. Testing Nano-particles were tested for antifungal activities. The results showed nano - particles from Ch. brasiliense gave effectively significantly inhibition of colony growth and spore production which the ED <inf>50</inf> values were 1.08 µg/ml, and 8.68 µg/ml respectively. Application of Nano - particles to control the P. palmivora causing root rot disease of durian in pot experiment was successfully done. The results showed nano-particles from Ch. brasiliense reduced the root rot disease on durain of 40%. The nano-particles from Ch. brasilense gave significiantly high plant growth which were 79.5 cm when compared to the non-trated control.
Some of the metrics are blocked by your
consent settings
Nano-particles from Cheatomium brasiliense against brown spot of rice
(2018-12-01)
Vareeket, R.
;
Soytong, K.
;
Kanokmedhakul, S.
;
Kanokmedhakul, K.
Chaetomium brasiliense was used in this study to control rice brown spot disease pathogen, Drechslera oryzae. The result of bi-culture test showed that Ch. brasiliense gave the hightest percentage of growth inhibition at 26.38% and had the highest spore inhibition at 23.81%. In crude extracts test, it was found that crude methanol extract obtained from Ch. brasiliense at 1000 ppm showed the highest inhibitory effect on colony growth and spore production. Ch. brasiliense gave a growth inhibition and spore inhibition rates at 83.50 and 99.78% respectively. The effective dose (ED <inf>50</inf> ) on growth and spore inhibition of Ch. brasiliense was 80.54 and 0.35 µg/ml, respectively. It was also found that Nano-particles obtained from crude methanol extract of Ch. brasiliense (nano CBM) at 10 ppm had the best inhibitory effect in terms of growth and spore inhibition. Nano CBM can inhibit the growth at 70.00% and spore production at 79.92%. The ED <inf>50</inf> values for spore inhibition of Ch. brasiliense was 2.86 µg/ml.
Some of the metrics are blocked by your
consent settings
Nano-particles from Chaetomium lucknowense to inhibit rice blast pathogen causded by Pyricularia oryzae in pot experiment
(2018-12-01)
Song, J. J.
;
Soytong, K.
;
Kanokmedhakul, S.
;
Kanokmedhakul, K.
Nano-particles derived from Chaetomium lucknowense proved to be antagonized Pyricularia oryzae causing rice blast disease var. RD57. Result showed that nano-ECL, nano-MCL and nano-HCL expressed antifungal activities against P. oryzae (rice blast disease) at the ED <inf>50</inf> values were 82, 114, 181ppm, respectively. In pot experiment, the nano-CL gave significantly better to control rice blast than the chemical fungicide (Tricyclazole) in rice var. RD57. Rice blast disease showed that nano-CL gave the highest reduction of 54 %, when compared to the chemical fungicide that the disease decreased 29.26 %. Application of nano-CL gave the highest plant strands of 87.62 cm when compared to Tricyclazole (74.91 cm). Nano-particles from C. lucknowense is being developed to be nano-elicitors for plant immunity.
Some of the metrics are blocked by your
consent settings
Nano-particles derived from Chaetomium elatum against Phytophthora rot of durian
(2018-12-01)
Thongkham, D.
;
Soytong, K.
;
Kanokmedhakul, S.
;
Kanokmedhakul, K.
Durian are the economically fruit trees in Thailand. The important problem of durian is root rot disease caused by Phytophthora palmivora. This study was used Chaetomium elatum to control the P. palmivora causing root rot disease of durian by dual culture method, crude extract test and nano particles test derived from Ch. elatum. Dual-culture test showed that Ch. elatum gave efficiency to inhibit of spore and colony growth of P. palmivora which were 46.13 and 38.89%, respectively. Testing efficacy of crude extract from Ch. elatum to control P. palmivora found that crude ethyl acetate from Ch. elatum gave significantly highest against pathogen of P. palmivora at the concentration of 1000 ppm which the ED <inf>50</inf> of 175.31 ppm. Nano particles testing, nano particles of crude hexane, ethyl acetate and methanol from Ch. elatum showed the ED <inf>50</inf> values of 3.49, 3.47 and 3.41 ppm.
Some of the metrics are blocked by your
consent settings
Application of nano-particles derived from chaetomium elatum che01 to control pyricularia oryzae causing rice blast
(2018-11-01)
Song, J. J.
;
Kanokmedhakul, S.
;
Kanokmedhalkul, K.
;
Soytong, K.
Pyricularia oryzae causing rice blast was isolated and proved for pathogenicity. Chaetomium elatum ChE01 was proved to be antagonized P. oryzae in bi-culture antagonistic test which averaged inhibition of 60.40 % within 15 days. Fungal metabolites from C. elatum ChE01 were extracted and tested to inhibit P. oryzae. Results showed that crude ethyl acetate expressed antifungal activity against P. oryzae which the effective dose <inf>50</inf> (ED <inf>50</inf> ) was 231 ppm., followed by crude methanol and crude hexane which the ED <inf>50</inf> were 460 and 2,122 ppm. respectively. It was shown that nano-CCE gave the highest inhibition P. oryzae which the ED <inf>50</inf> was 8.25 ppm, and followed by nano-CEM and nano-CEH which the ED <inf>50</inf> values were 11.21 and 65.52 ppm, respectively. Further research findings are investigated in pot and field experiments.
Some of the metrics are blocked by your
consent settings
Antifungal metabolites from antagonistic fungi used to control tomato wilt fungus Fusarium oxysporum f. sp. lycopersici
(2011-12-28)
Sibounnavong, P.
;
Charoenporn, C.
;
Kanokmedhakul, S.
;
Soytong, K.
Chaetomium elatum strain ChE01, Chaetomium lucknowense strain CLT01 and Emericella rugulosa strain ER01, which were isolated from soil in Thailand, effectively controlled the most virulent isolate of Fusarium oxysporum f. sp. lycopersici NKSC02 causing wilt of tomato (Lycopersicon esculentum var Sida). Two antifungal substances were purified: Chaetoglobosin-C from Ch. elatum and Ch. lucknowense and tajixanthone from E. rugulosa. Chaetoglobosin-C showed greater antifungal activity against F. oxysporum f. sp. lycopersici, with an effective dose (ED <inf>50</inf>) of 5.98 μg/ml, compared with tajixanthone (ED <inf>50</inf> of 167 μg/ml). These results suggest that the disease control mechanism of these antagonistic fungi involves antibiosis. The inoculating tomato seedlings var. Sida with conidia of F. oxysporum f.sp. lycopersici, mixed with either a solution of chaetoglobosin-C or tajixanthone showed no wilt symptom. The conidia of F. oxysporum f. sp. lycopersici treated with these two compounds appeared abnormal and lost pathogenicity. © 2011 Academic Journals.
Some of the metrics are blocked by your
consent settings
Evaluation of bio-agent formulations to control Fusarium wilt of tomato
(2010-09-06)
Charoenporn, C.
;
Kanokmedhakul, S.
;
Lin, F. C.
;
Poeaim, S.
;
Soytong, K.
The ED<inf>50</inf> value of antagonistic substance from Chaetomium globosum N0802 extracted with hexane was 157 μg/ml. This gave the highest inhibition of conidial production of Fusarium oxysporum f. sp. lycopersici causing tomato wilt var Sida. Crude hexane from Chaetomium lucknowense CLT and crude methanol from Trichoderma harzianum PC01 gave ED<inf>50</inf> values of 188 and 192 μg/ml. It clearly demonstrated that antagonistic substances from all tested fungi could be deformed and this could break the conidial cells. The bio-agent formulations namely N0802, CLT and PC01 gave significantly highest disease reduction of tomato wilt which were 44.68, 36.28 and 41.01%, respectively, followed by prochoraz (21.95%). All tested bio-agent formulations could significantly increase the yield of tomato when compared to prochoraz and inoculated control. It is concluded that C. globosum, C. lucknowense and T. harzianum developed as bio-agent formulations namely N0802, CLT and PC01 and showed their abilities to control tomato wilt. © 2010 Academic Journals.
Some of the metrics are blocked by your
consent settings
Application of Chaetomium species (Ketomium®) as a new broad spectrum biological fungicide for plant disease control: A review article
(2001-12-01)
Soytong, K.
;
Kanokmedhakul, S.
;
Kukongviriyapa, V.
;
Isobe, M.
Research and development of biological control agents for use against plant diseases have been undertaken for several years in both government and private sectors, as natural agents are needed to take the place of chemical fungicides. The problem associated with the use of hazardous chemicals for disease control has received increasing attention worldwide, due to the fact that pathogens become resistant to chemical fungicides and the resulting environmental pollution and ecological imbalances. The implementation of practical integrated biological control technology to control plant diseases has been successfully introduced to growers in China, Philippines, Russia, Thailand and Vietnam by using new broad spectrum biological fungicides from Chaetomium (Thailand Patent No. 6266, International Code: AO1N 25/12 and registered as Ketomium® mycofungicide). Since 1989, the biological product has been developed and improved from 22-strains of Chaetomium cupreum CC01-CC10 and Chaetomium globosum CG01-CG12 in the form of pellet and powder formulation. The formulation has successfully been applied to infested field-soils with integrated with cultural control measures and organic amendments for the long-term protection of Durian (Durio zibethinus L.) and Black Pepper (Piper nigram L.) caused by Phytophthora palmivora, Tangerine (Citrus reticulata Blanco) caused by Phytophthora parasitica and Strawberry (Fragaria spp.) caused by Phytophthora cactorum, Wilt of Tomato (Lycopersicon esculentum L.) caused by Fusarium oxysporum f. sp. lycopersici and Basal rot of Corn (Zea mays L.) caused by Sclerotium rolfsii against Phytophthora rot. Bio-control technology of plant diseases has successfully been demonstrated, either completely or partially, when integrated with other control measures for appropriate disease management. Biological products are useful, not only for the protection against plant diseases, but can also be used for curative effects of plant diseases, and also promote plant growth.

KMITL

Browse

Filters

Settings

Sort By

Results per page

Search Results