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    The Effects of Hormone Diets with Different 17β-Estradiol Levels on Growth and Feminization in Long-Whiskered Catfish (Mystus gulio) Larvae Using Conventional and Microencapsulated Feed
    (2026-01-01)
    Dokkaew, Sahabhop
    ;
    Songdum, Kritchavat
    ;
    Prachom, Noratat
    ;
    Boonyung, Wiwiththanon
    ;
    Kitikiew, Suwaree
    Feminization is an important biotechnological approach in aquaculture for species in which females exhibit superior growth and higher market value. The long-whiskered catfish (Mystus gulio), a euryhaline species cultivated in both monoculture and co-culture systems, contributes to sustainable aquaculture by grazing on uneaten feed and maintaining pond cleanliness. This study evaluated the effects of dietary 17β-estradiol (E2) at 0, 10, 30, and 60 mg/kg, incorporated into conventional and microencapsulated feeds, on the feminization and early growth of M. gulio larvae. Treatments were administered during the weaning stage for 14 and 21 days under controlled rearing conditions. Results showed that larvae fed microencapsulated feed containing 60 mg/kg E2 achieved the highest specific growth rate (26.91 ± 1.92%/day), feed efficiency (164.76 ± 33.23%), and feminization success (99.73 ± 0.04%). Hormonal assays confirmed elevated estradiol and reduced testosterone levels, consistent with ovarian development observed in histological sections. Gene expression analysis further supported these findings through the significant upregulation of cyp19a, erb1, and erb2 mRNA levels. Overall, this study demonstrates that microencapsulated hormone feeding is an effective and environmentally responsible strategy for achieving monosex female populations in M. gulio, enhancing productivity, reproductive performance, and sustainability in aquaculture systems.
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    Item type:Publication,
    Status of Aquarium Fish and Plants in Asia
    (2026-01-01)
    Kantha, Phunsin
    ;
    Dokkaew, Sahabhop
    ;
    Sirisuay, Soranuth
    ;
    Kitikiew, Suwaree
    ;
    Nhan, Hua Thai
    The ornamental aquarium market thrives on live organisms from wild captures and breeding programs, with over 90% of freshwater species and half of traded species sourced from breeding initiatives. Breeding marine species such as clownfish, marine angelfish, tang/surgeonfish, and corals 20has recently expanded, reflecting a shift towards sustainability and ecological conservation. In Asia, aquarium hobbyists blend ornamental fish and aquatic plants into miniature aquatic ecosystems, marking aquascaping’s evolution from traditional fishkeeping to intricate underwater landscapes. Rich biodiversity, complex layouts, and integration of natural processes like photosynthesis and the nitrogen cycle characterize Asian aquascaping. International recognition of Asian aquascapers at competitions like the World Aquascape Championships (IAPLC) underscores the global community’s pivotal role in the art’s continuous growth and innovation. This chapter further examines breeding techniques, ecological roles, and aesthetic contributions of aquatic life in Asia, highlighting sustainable practices and responsible stewardship essential for industry viability. Emphasizing ecological conservation, the chapter advocates for a balanced approach respecting both artistic expression and environmental sustainability, urging continued research, collaboration, and active community involvement to ensure an ecologically responsible future for this unique intersection of art and nature.
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    Item type:Publication,
    The Effect of Light-Emitting Diodes (LEDs) on the Development of Duckweed (Lemna minor) in Co-Culture with Red Tilapia (Oreochromis spp.)
    (2023-05-01)
    Luo, Jie
    ;
    Chien, Yew Hu
    ;
    Taparhudee, Wara
    ;
    Kitikiew, Suwaree
    ;
    Kantha, Phunsin
    The effects of artificial light source and photoperiod on duckweed (Lemna minor) growth and the impact of duckweed on red tilapia (Oreochromis spp.) growth in an aquaponic culture are not well understood. This study aimed to investigate: 1) effects of light source and photoperiod on growth of duckweed and water quality, and 2) the effects of feeding red tilapia with different ratios of duckweed: artificial feed. In Phase I, three different artificial light sources (LED white light, T5 fluorescent white light, and LED blue light) were used and then a consecutive experiment was conducted using LED white light with three different photoperiods: 24:0, 16:8, and 12:12 (light:dark). In Phase II, red tilapia were fed with three feeding regimes made up of different percentages (by weight) of duckweed (0%, 5%, and 10%) to artificial pellet feed. Overall, our results suggest that using LED white light with a photoperiod of 16:8 (light:dark) was effective for maximizing duckweed growth in aquaponic culture and consequently resulted in lowest total ammonia nitrogen (TAN) and NO<inf>2</inf>-, and that replacing pellet feed with duckweed at 5% or 10% can increase the growth rate of red tilapia.