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Author: search.filters.author.Kanthang, Supha

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    Tobacco randomly inserted TT8 differenly enhance light signals and flavonoid accumulation
    (2015-08-18)
    Sompornpailin, Kanokporn
    ;
    Kanthang, Supha
    The individual lines of tobacco over-expressing TT8, a bHLH gene, were constructed and cultured under tissue culture condition radiating with photosynthetically activation radiation (PAR) or PAR+UVA. They were compared to wild type (WT). Leaf of treated plants was extracted and analyzed for flavonoid accumulations using a spectrophotometer. The extract of TT8 plants significantly contained flavone, flavonol and anthocyanin level, higher than the WT extract did. The petal extracts of mature transgenic under PAR had a similar absorbance profile of each substance, but these extracts had higher flavonoid contents than the leaf extracts did. All flavonoid subgroups and p-coumaric acid biosynthesis were significantly enhanced after the additional UVA radiation to plant. This UVA condition slightly stimulated an accumulation of these substances in normal plant. Some transgenic greatly increased flavonoid accumulation in responding to PAR+UVA, but the others were slightly different compared to WT. The distinct insertion site is directly affected TT8 gene expression. Transgenic seeds had a dark brown color more than WT seed, which indicated high content of polymer flavonoids (proanthocyanins). This over-expressing TT8 in transgenic tobacco may directly or indirectly enhance the signal transductions of PAR and UVA and raise up flavonoid accumulation.
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    Tobacco expressing PAP1 increases the responses to PAR and UV-A by enhancing soluble sugars and flavonoids and elevating plant protections
    (2015-04-01)
    Sompornpailin, Kanokporn
    ;
    Kanthang, Supha
    Five lines of transgenic tobacco over-expressing Production of Anthocyanin Pigment 1 (PAP1) cDNA were analysis of metabolic response against the radiation and their protection of the plant under tissue culture condition. PAP1 transgenic and wild type (WT) plants were treated with the radiations of photosynthetically activate radiation (PAR) or PAR combined with UV-A. All lines of transgenic significantly increased in amounts of p-coumaric acid, naringenin apigenin more than WT under both treatments. Additional UV-A radiating to plant rose up kaempferol content in WT plant (1.5 times) and in PAP1 transgenics (1.8 times). These transgenic plants treated under both conditions had also increased anthocyanin substances (pelargonidin) with significant value after compared to WT. Content of total soluble sugar (TSS) was related to the content of total flavonoids in transgenic. PAR combined with UV-A had a lower induction of the electrolyte leakage percentage and malondialdehyde (MDA) level in the transgenic leaf tissue compared to WT tissue. The metabolic substance levels were considered on its protection of plant cells. In transgenic tissue, the enhancement of apigenin level strongly diminished the increase level of electrolyte leakage while the levels of TSS, p-coumaric acid and naringinin less affected. Moreover, the increase levels of kaempferol and pelargonidin associated with the decrease level of MDA, while the TSS level reversely responded. The PAP1 transgenic increased response of light by adaptation of their metabolites (TSS, p-coumaric acid and flavonoids) consequently enhance parameter indicating protections of the cell.
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    Increasing plant flavonoid biomaterials in response to UV-A light
    (2013-10-29)
    Kanthang, Supha
    ;
    Sompornpailin, Kanokporn
    Flavonoid biomaterials have a protecting function from various stresses. We examined the flavonoid biosynthesis in plant treated under visible light (VL) and additional UV-A light. The transgenic tobacco containing PRODUCTION OF ANTHOCYANIN PIGMENT 1 (PAP1) cDNA, involved in flavonoid biosynthesis from Arabidopsis thaliana, were used for studying the flavonoid biosynthesis under both light conditions comparing to non transgenic tobacco. The flavonoid biomaterials were extracted with acidic methanol and water solvent from treated plant leaves. The absorbance of each biomaterial in the extract was measured under specific wavelength using a spectrophotometer. Additional UV-A radiated to non transgenic and transgenic tobacco affect the increasing of p-coumaric acid, naringenin, apigenin and kaempherol biomaterials from themselves grown under VL (approximately 120-130%). However, PAP1 transgenic tobaccos under additional UV-A radiation enhance the accumulation of these biomaterials up to160-180% higher than non transgenic tobaccos grown under VL condition. Moreover, PAP1 transgenic tobacco radiated with UVA light also significantly increased pelargonidin biomaterial. PAP1 transgenic tobaccos had a similar phenotype with non transgenic tobaccos but the color of fully expanding flower was more pink intensity than non transgenic. © (2013) Trans Tech Publications, Switzerland.