KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/123456789/1

Browse

Search Results

Now showing 1 - 9 of 9

Some of the metrics are blocked by your
consent settings
Stable Nanoemulsion System Development Enabling the Topical Delivery of Synechococcus-Derived Peptides
(2026-03-01)
Keawbankrud, Wannisa
;
Nakyai, Wongnapa
;
Phunpruch, Saranya
;
Sangtanoo, Papassara
;
Karnchanatat, Aphichart
Marine microalgae including Synechococcus sp. VDW offer the potential to serve as a source of bioactive peptides offering valuable antioxidant and antimelanogenic qualities for use in the pharmaceutical and cosmetics industries. At present, however, the use of such peptides is challenging due to their poor physicochemical stability. This research therefore sought to achieve the production and characterization of a stable nanoemulsion system based upon the use of synthetic Synechococcus-derived peptides through the process of high-pressure homogenization (HPH). Preparation of the nanoemulsions involved the use of Tween-80 and caprylic/capric triglyceride at a pressure of 7,500 psi to perform homogenization for varying durations of 15, 30 and 45 min. Using the optimized formulation with 0.1% w/w peptide for a time of 45 min resulted in 122.16 nm droplets while the zeta potential was −80.09 mV and the PDI (polydispersity index) value was 0.13. Colloidal stability could be considered high, while physical stability under thermal cycling, centrifugation and freeze-thaw cycles was very good, with no phase separation. For all testing intervals, the viscosity and refractive index were stable. It can thus be argues that the HPH approach is suitable to produce peptide-loaded nanoemulsions offering good stability and useful physicochemical characteristics. The developed nanoemulsion system has been optimized to offer potential for applications involving the transdermal delivery of marine peptides in the cosmeceutical sector and for a range of dermal therapies.
Some of the metrics are blocked by your
consent settings
Life cycle assessment of spray-drying encapsulation of crude peptides produced from defective green coffee beans
(2025-03-01)
Hunsub, Panusorn
;
Ngamprasertsith, Somkiat
;
Prichapan, Nattapong
;
Sakdasri, Winatta
;
Karnchanatat, Aphichart
The use of agricultural waste for the production of value-added ingredients was investigated. Defective green coffee beans were utilized as alternative feedstocks for the production of peptides. The crude peptides were produced by enzymatic hydrolysis, followed by membrane filtration and centrifugation. Subsequently, hydrolysate peptides were encapsulated via spray drying with maltodextrin as the carrier, facilitating their use as a functional ingredient in food supplements. This study aimed to produce hydrolysate peptides from defatted defective green coffee beans by supercritical carbon dioxide with ethanol as a cosolvent. Additionally, their environmental impacts (gate to gate) were evaluated and analyzed using the life cycle assessment approach. Data obtained from pilot-scale production of 10 L of oil extract and 50 L of peptide extract were used to examine the environmental consequences of the various treatments. Two scenarios of downstream processing were examined for equivalent antioxidant activity: spray-drying without maltodextrin (Scenario 1) and spray-drying with maltodextrin encapsulation (Scenario 2). Scenario 2 consumed less electricity than Scenario 1 due to its shorter operating time and lower environmental impact compared to Scenario 1. Additionally, its encapsulated form is suitable for antioxidant retention and the storage of functional ingredients. The greatest environmental impact arose from supercritical carbon dioxide with ethanol extraction, which affected 8 out of 15 midpoint categories, followed by spray dry encapsulation, which affected 7 out of 15 midpoint categories. Solutions for improvement, such as carbon dioxide and/or ethanol recycling, and alternative heat and electricity sources are also discussed.
Some of the metrics are blocked by your
consent settings
Bee pollen peptides as potent tyrosinase inhibitors with anti-melanogenesis effects in murine b16f10 melanoma cells and zebrafish embryos
(2024-12-01)
Sangtanoo, Papassara
;
Srimongkol, Piroonporn
;
Saisavoey, Tanatorn
;
Puthong, Songchan
;
Buakeaw, Anumart
One important functional food ingredient today, valued for its health properties and ability to prevent disease, is bee pollen, which comprises a combination of nectar, pollen from plants, and the secretions of bees. In this research, the tyrosinase (TYR) inhibiting abilities of the peptides derived from bee pollen protein hydrolysates are investigated. Various proteases were utilized to generate these peptides, followed by testing at different concentrations. Tyrosinase inhibition activity was detected in all cases, while the hydrolysate drawn from 5.0% w/v neutrase exhibited the best IC<inf>50</inf> value and was thus investigated further via ultrafiltration to separate the active fractions. The highest potential for tyrosinase inhibition was recorded for the fractions below 0.65 kDa. Subsequent purification steps via SEC and RP-HPLC led to the identification of the VDGYPAAGY (named VY-9) peptide via LC-Q-TOF-MS/MS in fraction F<inf>1–2</inf>, known for its non-toxic and hydrophobic characteristics albeit poor water solubility. The synthesized VY-9 peptide demonstrated competitive inhibition, with IC<inf>50</inf> values of 0.55 ± 0.03 µM for mono-phenolase and 2.54 ± 0.06 µM for di-phenolase activities, as confirmed by molecular docking analysis revealing dominant hydrogen bond interactions with TYR. Effective concentrations of 0.2–1.6 µM of VY-9 showed negligible cytotoxicity in B16F10 cells. Melanin synthesis suppression was examined via qRT-PCR, and western blot in MITF, TYR, TRP-1, and TRP-2. Cell death in zebrafish embryos was evaluated in vivo using a toxicity assay which revealed no significant influence from VY-9, while anti-melanogenic effects were observed when the concentration was 4 µM, suggesting bee pollen-derived peptides’ potential in cosmetic and pharmaceutical depigmentation applications.
Some of the metrics are blocked by your
consent settings
Optimization, isolation, identification and molecular mechanisms in B16F10 melanoma cells of a novel tyrosinase inhibitory peptide derived from split gill mushrooms
(2024-10-01)
Arnamwong, Sarinya
;
Kuptawach, Kittisak
;
Sangtanoo, Papassara
;
Srimongkol, Piroonporn
;
Saisavoey, Tanatorn
Hyperpigmentation often arises from an imbalance in melanogenesis, primarily due to the overexpression of tyrosinase (TYR). While the inhibition of TYR presents a common approach to skin whitening, it can lead to undesirable side effects. Thus, there is growing interest in safe and natural alternatives for TYR inhibition. Bioactive compounds and peptides sourced from split gill mushrooms hold promise in this regard. This study aims to optimize the conditions for papain-mediated hydrolysis of split gill mushroom protein to inhibit TYR activity, utilizing response surface methodology (RSM) and central composite design (CCD). Optimal conditions were determined at a temperature of 46.70 °C, a hydrolysis time of 217.09 min, and an enzyme-to-substrate ratio (E/S) of 1.1%. Under these conditions, the resulting hydrolysates exhibited significant TYR inhibition, with an IC<inf>50</inf> value of 117.86 μg/mL and a degree of hydrolysis (DH) of 87.97%. Further purification via ultrafiltration and RP-HPLC yielded a peptide, Tyr-Ala-Ser-Ile-Leu-Leu (YASILL or YL-6), identified through LC-Q-TOF-MS/MS, which competitively inhibited TYR. YL-6 demonstrated an IC<inf>50</inf> value of 3.97 mM for mono-phenolase activity and 6.75 mM for di-phenolase activity. Molecular docking analysis revealed hydrogen bonds and hydrophobic interactions between TYR and YL-6. Treatment of B16F10 cells with YL-6 across concentrations ranging from 10-3000 μM showed no cytotoxic effects.The inhibition of melanin synthesis was investigated via qRT-PCR along with Western blot in MITF, TYR, TRP-1, and TRP-2. The results obtained in this research may prove significant in guiding the development of commercially viable cosmetic products to whiten the skin.
Some of the metrics are blocked by your
consent settings
Synechococcus marine microalgae peptide: Melanogenesis inhibition in cellular and zebrafish models
(2024-08-01)
Srimongkol, Piroonporn
;
Sangtanoo, Papassara
;
Saisavoey, Tanatorn
;
Puthong, Songchan
;
Buakeaw, Anumart
In this study, the AILQSYSAGKTK (named AK-12) peptide, also known as AK-12, from the Synechococcus marine microalgae cell extract is examined to determine the mechanism by which tyrosinase inhibition takes place. According to the docking simulation, it is expected that the peptides bind and interact at the tyrosinase active site, with the potential to support the inhibition of tyrosinase. For testing, the required peptides were first synthesized, before the results revealed tyrosinase inhibitory activity for which the respective IC<inf>50</inf> values for mono- and di-phenolase activities were 489.71 ± 0.01 μM, and 765.57 ± 0.01 μM. The characteristics of the different competitive types were shown in a Lineweaver-Burk plot. For the treatment of B16F10 cells, peptide concentrations of 50–400 μM were selected, confirming the absence of cytotoxicity. When the peptide was introduced, both tyrosinase activity and the production of melanin were significantly inhibited. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was then used to assess the suppression of melanin synthesis in MITF (microphthalmia-associated transcription factor, TYR (tyrosinase), TRP-1 (tyrosinase-related protein-1) and TRP-2 (tyrosinase-related protein-2). An in vivo toxicity assay was also carried out to evaluate zebrafish embryo cell death, revealing that AK-12 did not significantly affect cell death, while strong anti-melanogenic activity was observed for the concentration of 50 μM. From these findings it could be concluded that the peptide in question may offer potential in future hyperpigmentation treatments.
Some of the metrics are blocked by your
consent settings
Effects of Defatting Pretreatment on Polysaccharide Extraction from Rambutan Seeds Using Subcritical Water: Optimization Using the Desirability Approach
(2024-07-01)
Nilmat, Kamonthip
;
Hunsub, Panusorn
;
Ngamprasertsith, Somkiat
;
Sakdasri, Winatta
;
Karnchanatat, Aphichart
Rambutan seeds are by-products generated from fruit-processing factories; the leftover seeds are buried in landfills, generating methane emissions. This work aimed to extract polysaccharides (POLS) from rambutan seeds by using subcritical water extraction (SWE). The effects of defatting pretreatment and operating parameters in SWE were investigated using a Box–Behnken design. The results show that defatting pretreatment significantly enriched the POLS yield, while it had no significant effect on the total sugar content. Using the desirability approach, the suitable feedstock for SWE was defatted rambutan seeds. The maximum desirability of 0.86 was found at a temperature range of 145–150 °C, an extraction time of 15 min, and a liquid–solid ratio of 10:1. The POLS yield and total sugar content were in the range of 52.33–55.63 g/100 g feedstock and 83.37–87.45 g/100 g POLS, respectively. The extracted POLS had an equivalent molecular weight of 413.70 kDa that could be used as an extender in plant-based products. In conclusion, the defatting pretreatment of rambutan seeds not only improved the POLS yield obtained via SWE but also generated additional lipids that could be utilized as an unconventional source of specialty fat.
Some of the metrics are blocked by your
consent settings
Production of Coffee oil and Bioactive Peptides from Spent Coffee Grounds via Supercritical Carbon Dioxide Extraction and Enzymatic Hydrolysis
(2024-04-01)
Hunsub, Panusorn
;
Ponmana, Kanokporn
;
Ngamprasertsith, Somkiat
;
Sakdasri, Winatta
;
Karnchanatat, Aphichart
Supercritical carbon dioxide (SCCO<inf>2</inf>) extraction was applied for recovering non-polar compounds in spent coffee grounds (SCGs) before enzymatic hydrolysis was performed. The SCCO<inf>2</inf> extracted oil yield of 11.93 wt% was observed at 30 MPa and 50 °C. The detectable volatile compounds in SCGs oil were aldehydes and flavor compounds classified as furans. Although the degree of hydrolysis of SCGs and defatted SCGs (DFSCGs) were not significantly different, the soluble protein of DFSCGs was higher than that of SCGs. Electrophoretic profiles of SCGs and DFSCGs comprise polypeptide bands at < 20 and ~ 24 kDa, which are mainly derived from 11S globulin subunits. After hydrolysis, the molecular masses of 4–20 and 24 kDa were virtually eliminated, thus releasing polypeptides with molecular masses < 4 kDa. Furthermore, the DFSCGs hydrolysate had higher total phenolic content and antioxidant capacity than that of SCGs hydrolysate. Pretreatment of SCGs with SCCO<inf>2</inf> enhances enzymatic accessibility, improves the quality of protein hydrolysate, and procures SCGs oil as a by-product. Graphical Abstract: (Figure presented.)
Some of the metrics are blocked by your
consent settings
Sequential Process to Valorisation of Rambutan Seed Waste by Supercritical CO2-Ethanol and Subcritical Water Extractions
(2024-01-01)
Nilmat, Kamonthip
;
Ngamprasertsith, Somkiat
;
Sakdasri, Winatta
;
Karnchanatat, Aphichart
;
Sawangkeaw, Ruengwit
This work demonstrates the extraction of crude lipid and crude polysaccharide from rambutan (Nephelium lappaceum Linn.) seed using supercritical CO2 with ethanol as co-solvent (SCE) and subcritical water (SCW) extractions, respectively. As dried rambutan seeds contain 32.48 ± 1.07 g of extractable lipid/100 g dried sample, pre-extraction of 16.65% of the lipid content by a screw pressing machine improved the efficiency of SCE extraction. Moreover, the addition of ethanol as a co-solvent resulted in a fivefold improvement in phenolic compound extraction. The crude lipid extract had a total phenolic content of 163.81 ± 0.47 mg GAE/100 g extract. The SCW extraction was investigated by a Box-Behnken design using extraction yield and total sugar content as responses. The optimal condition was found at temperature range of 150–180°C and extraction time in the range of 15–60 min where both responses were maximized. The solid residue discharged from subcritical water extractor was identified as hydrochar because it contained rich oxygen functional groups. This zero-waste process produced 5.2 kg of SCCO2-extracted lipid, 16.2 kg of crude polysaccharide, and 37.7 kg of hydrochar from 100 kg of fresh rambutan seed.
Some of the metrics are blocked by your
consent settings
Sulfated polysaccharides derived from marine microalgae, Synechococcus sp. VDW, inhibit the human colon cancer cell line Caco-2 by promoting cell apoptosis via the JNK and p38 MAPK signaling pathway
(2023-01-01)
Srimongkol, Piroonporn
;
Songserm, Pajareeya
;
Kuptawach, Kittisak
;
Puthong, Songchan
;
Sangtanoo, Papassara
This study offers an examination of the physicochemical qualities of marine microalgae-derived sulfated polysaccharides (MMPS) from Synechococcus sp. VDW along with an investigation of their antioxidant and antitumor activities. On average, MMPS offered a molecular weight of 190.94 kDa with sulfate content of 16.83 %. There were shown to be four different monosaccharides contained within MMPS. The anticancer characteristics of human colon cancer cell lines (Caco-2) were also investigated. Flow cytometry revealed that MMPS triggered apoptosis at doses of 1 mg mL<sup>−1</sup>. Meanwhile, analysis of gene and apoptosis protein expression demonstrated that MMPS increased apoptosis in Caco-2 cells via the p38 MAPK and JNK signaling pathways. These findings contribute to our current understanding of how MMPS helps promote tumor suppression. In conclusion, the possibility that MMPS may possess anticancer qualities in the treatment of human colon cancer makes it an appealing candidate for anticancer polysaccharide combinations with chemotherapeutic agents and functional foods.

KMITL

Browse

Filters

Settings

Sort By

Results per page

Search Results