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Author: search.filters.author.Khunchuay, ChonnikarnHas files: No

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    A proper concentration of carbon black nanoparticles enhances growth of the regenerated vetiver grass
    (2017-01-01)
    Khunchuay, Chonnikarn
    ;
    Sompornpailin, Kanokporn
    Carbon black (CB) nanoparticles were used in enhancing efficiency of the plant regeneration process under tissue culture condition. The calli of vetiver grass (Vetiveria zizanioides L. Nash) were induced by using axillary bud as the explant. These calli were subjected onto plant regeneration medium, Murashige & Skoog (MS) medium supplemented with 1 mgL<sup>-1</sup> α-naphthalene acetic acid (NAA), 2 mgL<sup>-1</sup> 6-benzylaminopurine (BAP) and 2 mgL<sup>-1</sup> kinetin, adding 0-70 mgL<sup>-1</sup> concentrations of carbon black (CB) nanoparticles. After six week incubation, vetiver calli developed on the medium supplemented with 40 mgL<sup>-1</sup> CB nanoparticles showed the rather high frequencies of plant regeneration (93.75%), nevertheless no statistically different with other treatment did. Media containing CB nanoparticles in the range 40-60 mgL<sup>-1</sup> significantly affected on enhancing the average of shoot number per callus more than other CB concentration did. Especially, the average shoot lengths of plantlets derived from 40 and 50 mgL<sup>-1</sup> CB media (1.73 and 2.19 cm) are longer than those from non CB or other CB concentration media (0.88<sup>-1</sup>.05 cm) at the significant level. Adding CB nanoparticles in regeneration medium effects on enhancing electrical conductivity and decreasing the pH value. The concentration of CB nanoparticle effected the ratio of colloidal sizes which was found in the medium. The proper concentration of CB generates the optimum property of the regeneration medium which can promote the average of shoot number per callus, and enhanced shoot elongation of vetiver grass in the regeneration process.
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    Synergistic effects of BAP and kinetin media additives on regeneration of vetiver grass (Vetiveria zizanioides L. Nash)
    (2016-05-01)
    Sompornpailin, Kanokporn
    ;
    Khunchuay, Chonnikarn
    Regeneration efficiency was enhanced by adding auxin and cytokinin at different concentrations to the media. It was found that the two highest frequencies-90 and 85%-of callus induction occured when the buds were cultured in an MS medium supplemented with 2 mgL<sup>-1</sup> a-naphthalene acetic acid (NAA) plus 1 mgL<sup>-1</sup> N<sup>6</sup>-benzylaminopurine (BAP) (CIM2) and in the same medium supplemented with 2 mgL<sup>-1</sup> NAA plus 1 mgL<sup>-1</sup> kinetin (CIM5), respectively. When cultured further in a plant regeneration medium supplemented with 1 mgL<sup>-1</sup> NAA and 2 mgL<sup>-1</sup> BAP (PRM1), these calli regenerated with high efficiencies, 80% for calli grown in CIM2 and 85% for calli grown in CIM5. The low BAP concentration in CIM1 (2 mgL<sup>-1</sup> NAA and 0.5 mgL<sup>-1</sup> BAP) resulted in a low regeneration efficiency and fewer shoots per callus even though the PRM3 regeneration medium had a high concentration of cytokinins (2 mgL<sup>-1</sup> BAP and 2 mgL<sup>-1</sup> kinetin) . However, the calli cultured in CIM2 without kinetin but with a suitable level of BAP still regenerated with high efficiencies in PRM2 and PRM3 that contained 1 and 2 mgL<sup>-1</sup> kinetin, respectively. Similarly, when calli cultured in CIM5 without BAP but with kinetin were regenerated in PRM1 with BAP alone, they also regenerated with a high regeneration efficiency. These results showed that culturing a specific tissue in a medium with suitable concentrations of NAA and BAP or kinetin resulted in a large number of multiple shoots, and BAP and kinetin worked synergistically to produce a high plant regeneration efficiency.