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Item type:Publication, Synergistic effects of BAP and kinetin media additives on regeneration of vetiver grass (Vetiveria zizanioides L. Nash)(2016-05-01); Khunchuay, ChonnikarnRegeneration efficiency was enhanced by adding auxin and cytokinin at different concentrations to the media. It was found that the two highest frequencies-90 and 85%-of callus induction occured when the buds were cultured in an MS medium supplemented with 2 mgL<sup>-1</sup> a-naphthalene acetic acid (NAA) plus 1 mgL<sup>-1</sup> N<sup>6</sup>-benzylaminopurine (BAP) (CIM2) and in the same medium supplemented with 2 mgL<sup>-1</sup> NAA plus 1 mgL<sup>-1</sup> kinetin (CIM5), respectively. When cultured further in a plant regeneration medium supplemented with 1 mgL<sup>-1</sup> NAA and 2 mgL<sup>-1</sup> BAP (PRM1), these calli regenerated with high efficiencies, 80% for calli grown in CIM2 and 85% for calli grown in CIM5. The low BAP concentration in CIM1 (2 mgL<sup>-1</sup> NAA and 0.5 mgL<sup>-1</sup> BAP) resulted in a low regeneration efficiency and fewer shoots per callus even though the PRM3 regeneration medium had a high concentration of cytokinins (2 mgL<sup>-1</sup> BAP and 2 mgL<sup>-1</sup> kinetin) . However, the calli cultured in CIM2 without kinetin but with a suitable level of BAP still regenerated with high efficiencies in PRM2 and PRM3 that contained 1 and 2 mgL<sup>-1</sup> kinetin, respectively. Similarly, when calli cultured in CIM5 without BAP but with kinetin were regenerated in PRM1 with BAP alone, they also regenerated with a high regeneration efficiency. These results showed that culturing a specific tissue in a medium with suitable concentrations of NAA and BAP or kinetin resulted in a large number of multiple shoots, and BAP and kinetin worked synergistically to produce a high plant regeneration efficiency.