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Author: search.filters.author.Kittibanpacha, K.Has files: No

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    Efficiency of cytokinin for propagation of Gluta usitata (Na-pong3) in vitro
    (2018-12-01)
    Rakrawee, R.
    ;
    Kittibanpacha, K.
    ;
    Chareonsap, P. P.
    ;
    Poeaim, A.
    The result was explained an effect of Plant Growth Regulators (PGRs) for young seeds induction and shoot multiplication of Gluta usitata (Na-pong3) by plant tissue culture technique to preserve and increased plant propagation. The young seeds were induced by comparison to 0.5, 1, 1.5, 2 and 3 mg/L 6-Benzyl amino purine (BAP), Thidiazuron (TDZ) or meta-Topolin (mT) on Woody Plant Medium (WPM). The results showed the highest percentage of seed regeneration was 1.5 mg/L BAP which was100% germination. The shoots was highest induced by 0.5 mg/L BAP after culture for 6 weeks. The shoots were transferred to WPM medium containing with BAP, TDZ or mT for multiple shoot induction. The maximum number of shoots were observed on WPM medium combined with 1.5 mg/L BAP (about 6 shoots per explant) after subcultured for three to four times. The best of root induction was observed on WPM medium without PGRs.
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    Efficiency of antioxidant and absorbent on browning and the optimal factors of plant regeneration from young seed of gluta usitata (217 Mae Ka) by Tissue Culture
    (2018-11-01)
    Rakrawee, R.
    ;
    Kittibanpacha, K.
    ;
    Chareonsap, P. P.
    ;
    Poeaim, A.
    Gluta usitata (Anacardiaceae) is a lacquer tree and create lacquer varnish can be utilized in many aspects especially art and culture. Recently, its harvest faced troublesome and yields of varnish tree are decreased. Consequently, the conservation of plant has reduced, and this plant has become extinct. The experiment was chosen to use plant tissue culture technique to preserve and increase this plant. The experiment was compared the effect of Woody Plant Medium (WPM) combined with different concentration of antioxidants were 2, 4, 6, 8 g/L Poly Vinyl Pyrrolidone (PVP), 20, 30, 40, 60 mg/L ascorbic acid or citric acid and absorbents of 1, 2, 3 g/L activated charcoal to reduce browning of phenolic compound in the explants. The results showed that 2 g/L activated charcoal gave the lowest of browning on seed and medium (about 0.9±0.2 points). The addition of 40 mg/L ascorbic acid was generated the highest of shoots (approximately 1.88±0.4 shoots/seed) after culture for 4 weeks. in vitro propagation of seed germination on WPM supplemented with 0.5, 1, 1.5, 2 and 3 mg/L 6-Benzyl amino purine (BAP), Thidiazuron (TDZ) and meta-Topolin (mT) 3% sucrose and 0.2% phytagel was efficiently resulted. Other result revealed that 1.5 mg/L BAP, 2 mg/L TDZ and 2 mg/L mT gave the highest seed germination at 1.6±0.2, 1.8±0.2 and 1.6±0.2 shoots/seed, respectively. The best of plant growth regulators for seed germination was 2 mg/L of TDZ after 6 weeks.