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Author: search.filters.author.Klamchuen, AnnopSubject: search.filters.subject.Gluten

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    Enhancement of Electrochemical Detection of Gluten with Surface Modification Based on Molecularly Imprinted Polymers Combined with Superparamagnetic Iron Oxide Nanoparticles
    (2022-01-01)
    Limthin, Dalawan
    ;
    Leepheng, Piyawan
    ;
    Klamchuen, Annop
    ;
    Phromyothin, Darinee
    Novel molecularly imprinted polymers (MIPs) represent a selectively recognized technique for electrochemical detection design. This rapid and simple method prepared via chemical synthesis consists of a monomer crosslinked with an initiator, whereas low sensitivity remains a drawback. Nanomaterials can improve charge transfer for MIP surface modification in order to overcome this problem. SPIONs have semiconductor and superparamagnetic properties that can enhance carrier mobility, causing high sensitivity of electrochemical detection. In this work, surface modification was achieved with a combination of MIP and SPIONs for gluten detection. The SPIONs were synthesized via the chemical co-precipitation method and mixed with MIPs by polymerizing gluten and methyl methacrylate (MMA), presented as a template and a monomer. Magnetic MIP (MMIP) was modified on a carbon-plate electrode. The morphology of modified electrode surfaces was determined by scanning electron microscopy–energy-dispersive X-ray spectrometry. The performance of the MMIP electrode was confirmed by cyclic voltammetry, amperometry, and electrochemical impedance spectroscopy. The MMIP electrode for gluten detection shows a dynamic linear range of 5–50 ppm, with a correlation coefficient of 0.994 and a low detection limit of 1.50 ppm, which is less than the U.S. Food and Drug Administration requirements (20 ppm); moreover, it exhibits excellent selectivity, sensitivity, stability, and reproducibility.
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    Methyl methacrylate magnetic molecularly imprinted polymer for gluten determination
    (2019-01-01)
    Limthin, Dalawan
    ;
    Chattrairat, Kunanon
    ;
    Leepheng, Pitawan
    ;
    Wisutthipat, Sawita
    ;
    Gansa, Poompun
    The gluten protein is found in some rice and flour. The allergy of gluten, a little bit of gluten in diet cause long-term damage and dangerous to the body. Even tiny amounts of gluten in diet may bring enormous symptoms. The rapid and simple method for gluten detection is molecularly imprinted polymers (MIP) combined with electrochemical analysis. In addition, magnetic molecularly imprinted polymers (MMIP) as known as Fe3O4 magnetic nanoparticles have used in combination with electrochemical measurement as well to improve the sensitivity of detection. In this work, the MMIP was combined with electrochemical technique. The Fe3O4 magnetic nanoparticles were synthesized by chemical reaction and then encapsulated with methyl methacrylate (MMA) as a functional group for gluten detection. Dynamic light scattering measurement clearly illustrates the average size of as-synthesized Fe3O4 nanoparticles as low as 150 nm. Chemical bonding, morphology, crystal structure and magnetic properties were characterized by fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), X-ray diffractometer (XRD), and vibrating sample magnetometer (VSM), respectively.