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Author: search.filters.author.Klaypradit, WanwimolSubject: search.filters.subject.Calcium carbonate

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    Effects of cuttlebone powders and their structured calcium compounds against glucocorticoid-induced osteoporosis via MAPK signaling-mediated bone formation
    (2026-04-01)
    Woonnoi, Wanwipha
    ;
    Chinfak, Narainrit
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    Tanasawet, Supita
    ;
    Saetan, Jirawat
    ;
    Klaypradit, Wanwimol
    The effects of dried and calcined cuttlebone powders (DCB and CCB, respectively) and their structured calcium compounds (DCB-OH, DCB-Lac, DCB-CiMa, CCB-OH, CCB-Lac, and CCB-CiMa) on MC3T3-E1 osteoblasts were investigated in vitro . Cytotoxicity analysis using the MTT assay revealed that lower concentrations (10–100 μg/mL) of all compounds were non-toxic, whereas higher concentrations (250–1000 μg/mL) showed dose-dependent cytotoxic effects. Using dexamethasone-induced osteoporosis-like MC3T3-E1 cells, cuttlebone-derived compounds significantly enhanced matrix mineralization as well as alkaline phosphatase (ALP) mRNA expression and activity, indicating restoration of osteoblast function. Additionally, treatment with all cuttlebone compounds reduced dexamethasone-induced apoptosis, demonstrating cytoprotective effects. Gene expression analysis showed suppression of ER stress markers ( CHOP, GRP78, ATF4 ) by most compounds, suggesting attenuation of ER stress-related osteoblast dysfunction. Osteoblast-related genes linked to matrix remodeling and bone formation ( MMP2, MMP9, COL1A1 ) were modulated; treatments generally downregulated MMP2 and upregulated COL1A1 expression. Key osteogenic transcription factors and markers ( BMP4, OC, OSX, RUNX2 ) decreased by dexamethasone were significantly upregulated by cuttlebone compounds, supporting enhanced osteoblast differentiation. Protein phosphorylation studies revealed that these compounds decreased the expression of pERK and p-p38, pathways that are often associated with impaired osteogenesis under glucocorticoid stress. Collectively, these findings demonstrate that cuttlebone powders and their structured calcium compounds mitigate dexamethasone-induced osteoporotic changes in MC3T3-E1 cells by enhancing cell viability, promoting osteoblast differentiation, and reducing apoptosis and ER stress, effects that are associated with suppression of MAPK pathway activation. This finding suggests a possible role in alleviating glucocorticoid-induced dysfunction in MC3T3-E1 osteoblasts and indicates potential relevance for future development as functional food ingredients or nutraceuticals for bone health.
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    Effect of fortified calcium compounds from oyster shell on the quality of tapioca pearls
    (2025-01-15)
    Meeparn, Parinda
    ;
    Aenglong, Chakkapat
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    Ratanasumawong, Savitree
    ;
    Klaypradit, Wanwimol
    ;
    Kerdpiboon, Soraya
    This study aimed to determine the effect of calcium fortification from dried oyster shells (DOS) and calcined oyster shells (COS) at concentrations of 2, 4 and 6 %(w/w) on physical and chemical properties of tapioca pearls. The results showed that the optimal cooking time of TP-COS decreased compared to TP-DOS and TP (control). The TP-DOS and TP-COS exhibited a remaining calcium content ranging from 8.39 to 41.03 mg/g. During seven days of refrigerated storage, TP-COS showed delayed hardness along with decreases in both the enthalpy of gelatinization and retrogradation. The functional groups observed in TP-DOS and TP-COS showed varying intensities compared to TP. Morphology images depicted the distribution of DOS and COS within tapioca pearls, revealing that TP-DOS and TP-COS possessed a denser and more compact structure. The results suggest that COS fortification could improve the nutritional value and delay the change in the texture of tapioca pearls after storage.
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