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Author: search.filters.author.Kudan, SanyaSubject: search.filters.subject.Saccharomyces carlsbergensis RU01

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    Stimulation of beta-glucan production from Saccharomyces carlsbergensis RU01 by tannin
    (2021-10-01)
    Chotigavin, Natthaporn
    ;
    Yaiyen, Surachai
    ;
    Kudan, Sanya
    ;
    Sriphochanart, Wiramsri
    This study aimed to evaluate the tannin-stimulated production of β-glucan from Saccharomyces carlsbergensis RU01 in molasses medium. Central composite design was used for the experiment design. The optimum concentrations of molasses (X<inf>1</inf>), ammonium sulfate (X<inf>2</inf>), and tannin (X<inf>3</inf>), which produced the highest biomass, were determined. Optimization analysis revealed that the optimum concentration of substrates for biomass was 3% (w/v) of molasses, 0.1% (w/v) of ammonium sulfate and 0.1% (w/v) of tannin. The maximum biomass production was 2.64 g/L. Meanwhile, the experimental validation was 2.84±0.10 g/L, and the highest β-glucan production was 119.47 mg/g of dry cell weight. Carbohydrate content in yeast cell walls was detected by Congo red staining. The cell wall of yeast grown in the optimized medium with tannin showed higher intensity than that grown in yeast malt medium. These results suggested that tannin addition can enhance β-glucan production with a high β-1,3-glucans content in the cell wall of S. carlsbergensis RU01.
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    Increasing the Production of β-Glucan from Saccharomyces carlsbergensis RU01 by Using Tannic Acid
    (2021-08-01)
    Chotigavin, Natthaporn
    ;
    Sriphochanart, Wiramsri
    ;
    Yaiyen, Surachai
    ;
    Kudan, Sanya
    In this study, we increased β-glucan production from brewer’s yeast, Saccharomyces carlsbergensis RU01, by using tannic acid. High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone. The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid. The results showed that MDS medium supplemented with 0.1% w/v tannic acid significantly increased the dry cell weight (DCW), and the β-glucan production was 0.28±0.01% w/v and 11.99±0.04% w/w. Tannic acid enhanced the β-glucan content by up to 42.23%. β-Glucan production in the stirred tank reactor (STR) was 1.4-fold higher than that in the shake flask (SF) culture. Analysis of the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy showed that the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid had a higher proportion of polysaccharide than that of the control. In addition, β-glucans from brewer’s yeast can be used as prebiotic and functional foods for human health and in animal feed.