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Larvicidal efficacy and morphological abnormalities induced by plant essential oils against housefly (Musca domestica L.)
(2026-03-01)
Passara, H.
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Sittichok, S.
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Moungthipmalai, T.
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Laosinwattana, C.
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Thongsaiklaing, M.
The results showed that the lowest complete pupation rate was 43.67%, indicating a significant inhibition of normal metamorphosis. Additionally, 56.33% of third-instar larvae were classified as malformed larvae (ML), dying before pupation and exhibiting severe morphological and physiological abnormalities. These findings suggest that the star anise nanoemulsion interferes with key developmental pathways, preventing larvae from reaching the pupal stage. Due to its high effectiveness and plant-based origin, this nanoformulation offers a promising, environmentally friendly alternative to conventional chemical insecticides. It has potential for further development into a natural larvicidal agent for use in integrated pest management (IPM) strategies targeting housefly populations. Future research should explore the underlying mechanisms of action and assess the efficacy and safety of this nanoemulsion under real-world conditions to support its application in sustainable vector control.
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Delaying quality changes in ‘Namdokmai Sithong’ mango fruit during low-temperature storage using trisodium phosphate
(2024-06-01)
Ganjana, R.
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Kramchote, S.
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Laosinwattana, C.
This study explored the efficacy of trisodium phosphate (TSP) in maintaining the postharvest quality of ‘Namdokmai Sithong’ mangoes under low-temperature storage conditions. Employing a completely randomized design, mangoes were treated with TSP at 0 (control), 0.25, 0.50, and 0.75 g L-1 and stored at 6±2°C with 85±5% RH for 25 days. Quality assessments, conducted every five days, focused on firmness, peel color, total soluble solids (TSS), hydrogen peroxide (H2O2), titratable acidity (TA), and phenolic content. Results showed that while TSP did not influence firmness, it significantly delayed peel color changes and affected TSS, H2O2, TA, and phenolic levels. The 0.50 and 0.75 g L-1 concentrations were most effective, with no marked difference between them, suggesting a recommendation of 0.50 g L-1 TSP for optimal cost-efficiency in preserving mango quality.
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The effect of microfludization on characteristics and herbicidal potential of peppermint nanoemulsion on Amaranthus tricolor
(2024-01-01)
Dimak, J.
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Somala, N.
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Laosinwattana, C.
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Teerarak, M.
The natural herbicides are friendly environment and human health. Peppermint essential oil nanoemulsion was prepared by a high-energy emulsification method using microfluidization with a non-ionic surfactant Tween 60 and was used to inhibit germination of Amaranthus tricolor seeds. Droplet size of the nanoemulsion was reduced by increasing the pressure of microfluidization from 5000 to 20000 psi (from 130.2 to 69.8 nm). The highest pressure (20000 psi) found the smallest droplet size of the nanoemulsion that droplet size, PI value, and zeta potential were 69.8 nm, 0.277, and -44.17 mV, respectively. Also, each pressure formulation (5000, 10000, 15000, and 20000 psi) was evaluated for the pre-emergence herbicidal activities namely inhibition of seed germination and seedling growth, seed imbibition, and a-amylase of Amaranthus tricolor seed. The obtained results of herbicidal activities correlated with droplet size that the herbicidal activities increased when increasing the pressure of microfluidization. The nanoemulsion formulation of pressure at 20000 psi treatment solution showed the highest herbicidal activities. Thus, these results may promote the optimized nanoemulsion from peppermint essential oil using a microfluidization method as a natural pre-emergence herbicide to inhibit seed germination and seedling growth of A. tricolor.
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Allelopathic potential of secondary metabolites produced by Alternaria brassicicola and physiological mechanisms on Amaranthus tricolor
(2023-05-01)
Manichart, N.
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Somala, N.
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Laosinwattana, C.
The results revealed that all isolated fungal strains significantly reduced germination and early growth of amaranth compared to control. The original metabolites (1.00X) from the fungus coded UNK03, which was identified as a strain of Alternaria brassicicola (98.44% similarity, accession number LC440588.1), showed the highest inhibition rate and suppressed with the maximum inhibitory level (78.75%) compared to control. In a similar pattern, the growth of the tested plant was significantly reduced by the cultural filtrates by 70.45% and 89.38% in shoot and root, respectively. Based on efficacy in the seed bioassay, the A. brassicicola metabolites (CF03) were selected for exposure with the tested seed to determine the mechanism of action (MOA). The results show that the A. brassicicola metabolites inhibited seed imbibition and -amylase activity of the tested seed with dose-dependent effects, strongly suggesting the potential of the metabolites to inhibit seed germination and seedling growth. Therefore, this work indicates that the fungal metabolites contain potent herbicidal compounds that can potentially be used to control A. tricolor.
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Formulation and application of clove essential oil-based nanoemulsion against Ruellia tuberosa L.
(2023-02-01)
Somala, N.
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Laosinwattana, C.
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Dimak, J.
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Teerarak, M.
Clove essential oil, a volatile compound, plays a role in herbicidal action on broadleaf weeds. Synthesis and technology nanoemulsion-based herbicide help protect the effectiveness of oils. The present study focused on the formulation, characteristics of clove essential oil-based nanoemulsion, and their herbicidal activity against Ruellia tuberosa L. The nanoemulsion containing clove essential oil was produced using the high-energy emulsification method with Tween 85 (HLB 11) and Span 85 (HLB 1.8) as an emulsifier agent (non-ionic surfactant), enabling the performance of clove oil nanoparticle size. The characteristic of nanoemulsion after being prepared 1 day showed that the droplet size, polydispersity index (PI) and zeta potential value was 152.5 nm, 0.162 and -32.68 mV, respectively. Even after 28 days, these characteristics have changed slightly. To confirm the droplet size, transmission electron microscope (TEM) images verified the clove oil-based nanoparticle size. Pre-emergence herbicidal activity under laboratory tests showed that the nanoemulsion at a concentration of 100, 200, 400 and 800 µL L-1 of oil reduced the germination and seedling growth of the tested weed. Inhibitory effect was absolutely at 800 µL L-1 of clove oil. Post-emergence application of nanoemulsion at a various concentration of 2000, 4000, 8000 and 10000 µL L-1 of oil was sprayed on 2-4 true leaves stage of tested weed. After foliar-applied nanoemulsion 24 h, the results showed that chlorophyll and carotenoid content decreased. Moreover, an increase in relative electrolyte leakage (REL) and malondialdehyde (MDA) indicated membrane disruption. This research supported that the clove oil-based nanoformulation may have a strong phytotoxic effect on R. tuberosa L. The nanoemulsion from clove oil is friendly to the environment and promote natural herbicide production for sustainable weed control.
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Peppermint essential oil extends the vase life of the cut Dendrobium orchid ‘White Sanan’
(2023-01-01)
Teerarak, M.
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Saetiew, K.
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Laosinwattana, C.
The vase life of cut orchid flowers is significantly extended by preservative solutions containing silver compounds. However, these compounds are toxic and require careful handling and disposal. As a result, the demand is increasing for natural preservation materials such as plant essential oils as they are biodegradable, ecofriendly, and safe alternatives for enhancing vase life and postharvest cut flower quality. The objective of this investigation was to study the effects of an emulsion of glucose and peppermint essential oil on vase life and postharvest quality of the popular cut Dendrobium orchid ‘White Sanan’. The orchids were preserved in vase solutions of distilled water or 4% glucose + 0.005-0.02% peppermint essential oil. The essential oil treatment increased the vase life of the flowers and the inflorescences by more than 6 days longer than the control treatment. The combination of glucose and peppermint essential oil increased water uptake and the fresh weight of cut orchids and delayed wilting time and abscission of floral buds and open florets. Moreover, treatment with the vase solution containing 4% glucose + 0.005% peppermint essential oil resulted in the highest bud opening rate (86.9%). Our results suggest that applying 4% glucose and 0.005-0.02% peppermint essential oil is especially effective as a preservative solution for the Dendrobium orchid ‘White Sanan’.
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Antimicrobial properties of extracts from Carissa carandas L. Fruits and its application in chilled and frozen ground pork
(2019-01-01)
Pilasombut, K.
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Laosinwattana, C.
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Tuyen Nguyen, T. K.
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Ngamyeeesoon, N.
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Teerarak, M.
In vitro antimicrobial activities of Carissa carandas L. fruit extracts dissolved in water and their effect on biological qualities in ground pork during storage period for 8 days at 4 o C and at -20 o C for 12 weeks were investigated. The antimicrobial avctivity was carried out using agar well diffusion method against food pathogenic and spoilage bacteria. For antimicrobial testing, water extracts of C. carandas inhibited both pathogenic and spoilage bacteria including Salmonella Typhimurium TISTR 292, Staphylococcus aureus TISTR 118, Escherichia coli TISTR 780, Aeromonas hydrophila TISTR 1321, Pseudomonas fluorescens TISTR 358 and Bacillus coagulans TISTR 1447, at the concentration of 50 mg/ml. The water extract of C. carandas fruit was applied in ground pork. The ground pork samples were subjected to four treatments including control (non-treated), 0.2 g BHT/kg meat, 2.5 g and 5 g extracts /kg meat. Total microbial count, yeast/mold, psychrophilic bacteria and coliforms were determined in ground pork during storage time for 0, 2, 4, 6 and 8 days at 4 o C and frozen ground pork storage at -20 o C for 0, 4, 8, and 12 weeks. The results showed that pH was not different in each treatments. Total plate count and coliforms bacteria was decreased after adding 5 g extracts /kg meat at 6-8 days in chilled ground pork and up to 8 and 12 weeks in frozen ground pork. This finding indicated that C. carandas fruit extracts could be used for natural antimicrobial in ground pork for prolong shelf-life.
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A simple and rapid transformation of golden apple snail (pomacea canaliculata) shells to calcium carbonate, monocalcium and tricalcium phosphates
(2019-01-01)
Seesanong, S.
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Laosinwattana, C.
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Chaiseeda, K.
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Boonchom, B.
This study was designed to manage golden apple snail shells, the wastes created in large amount daily from the consumption of the meat of golden apple snail (Pomacea canaliculata) shells by transforming them to advanced compounds; calcium carbonate (CaCO3), monocalcium phosphate monohydrate [Ca(H2PO4)2·H2O], and tricalcium phosphate [Ca3(PO4)2]. They were successfully prepared by a rapid, simple, environmentally benign method using easily available and low-cost instrument. All synthesized samples were characterized by X-ray fluorescence, X-ray powder diffraction, FTIR spectroscopy and scanning electron microscopy to confirm the identities with the standard materials. The reproducibility and low-cost method suggest that it could be used in industry for a large-scale production of calcium carbonate, monocalcium phosphate monohydrate and tricalcium phosphate from golden apple snail shells as a replacement of natural mineral resources and be a good way to manage these shell wastes.
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In vitro antimicrobial properties of different solvent extracts from carissa fruts Carissa carandas L
(2018-12-01)
Pilasombut, K.
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Laosinwattana, C.
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Tuyen Nguyen, T. K.
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Teerarak, M.
In vitro antimicrobial activities of various solvent systems (0%, 25%, 50%, 75% and 100% ethanol in water) of carissa fruit extracts (Carissa carandas) was reported. Agar well diffusion, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and killing time were determined. The results found that absolute ethanol and 75% ethanol extracts inhibited all 11 strains of both pathogenic and spoilage bacteria at the concentration of 100 mg/ml. The absolute ethanol extract was selected as an optimal extraction solvent for the following study showed the strongest antimicrobial activity. The MIC values of absolute ethanol extract to inhibit Pseudomonas fluorescens TISTR 358, Staphylococcus aureus TISTR 118, Salmonella Typhimurium TISTR 292, Escherichia coli TISTR 780 were 25, 25, 50, and 50 mg/ml, whereas the MBC values to inhibit these bacteria were 100, 25, 50, and 100 mg/ml, respectively. Moreover, S. aureus TISTR 118 and S. Typhimurium TISTR 292 were completely killed at 20 and 6 minutes exposure time by absolute ethanol extract from C. carandas fruits at the concentration of 25 mg/ml and 50 mg/ml, respectively.
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Optimal extraction solvents use for extraction of Thunbergia laurifolia Linn. Leaves and its mode of action on weed control
(2018-12-01)
Thinh, N. H.
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Laosinwattana, C.
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Wichittrakarn, P.
Thunbergia laurifolia Lindl. leaves were extracted by various ratios (0, 25, 50, 75 and 100%) of ethanol in water as solvents for crude extraction. The highest crude extract yield was obtained by using 25% ethanol solvent with three time extractions. The inhibitory effect of each extract on seed germination and seedling growth of Amaranthus gracilis was investigated. The results indicated that the 100% ethanol extract expressed the best inhibitory effect. At concentration of 1250 ppm, the 100% ethanol extract completely inhibited seed germination of A. gracilis. The 100% ethanol extract also inhibited α-amylase activities in A. gracilis seed during seed germination. However, the extract does not show effect on seed imbibition. The inhibition of induction of α-amylase increased with increasing concentration of the 100% ethanol extract.

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