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Author: search.filters.author.Lee, Jung Sook

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    Item type:Publication,
    Genomic insights into arsenic and antibiotic resistance in Comamonas thiooxydans strains F1-6 and A7-5
    (2026-04-01)
    Sitlaothaworn, Kanchana
    ;
    Phongsopitanun, Wongsakorn
    ;
    Chitpirom, Kitja
    ;
    Kingkaew, Engkarat
    ;
    Lee, Jung Sook
    Two arsenic-resistant, Gram-negative, aerobic rod-shaped bacterial strains, F1-6 and A7-5, which were isolated from agricultural soil in Thailand, were systematically studied. They grew at 30 °C, pH 5–11, and in 3% (w/v) NaCl. Ubiquinone with eight isoprene units (Q-8) and the cellular fatty acids C1<inf>6:0</inf>, C<inf>18:1</inf> ω7c, and C<inf>17:0</inf> CYCLO were the major components. Phylogenetic analysis via 16S rRNA gene sequences revealed that strains F1-6 and A7-5 were affiliated with the genus Comamonas and closely related to C. thiooxydans DF2<sup>T</sup> and C. testosteroni KCTC 2990<sup>T</sup>, with 99.79% and 99.86% similarity, respectively. The average nucleotide identity and digital DNA-DNA hybridization values between F1-6 and C. thiooxydans DF2<sup>T</sup> were 97.89% and 85.7%, respectively, whereas those between A7-5 and C. thiooxydans DF2<sup>T</sup> were 97.23% and 81.3%, respectively. Thus, both the F1-6 and A7-5 were identified as C. thiooxydans. The draft genome sizes of F1-6 and A7-5 were 5.2 and 5.3 Mb, comprising 87 and 84 contigs, with DNA G+C contents of 61.5% and 61.4%, respectively. The genomes of both strains contained ars cluster genes and many genes for growth and resistance to heavy metals and antibiotics, similar to those of C. testosteroni ATCC 11996<sup>T</sup>, C. thiooxydans CNB-1 substr. CNB-2 Chr, and C. terrae NBRC 106524<sup>T</sup>. Nevertheless, the acr3, qacG, and vanH genes in the vanO cluster were found only in C. terrae NBRC 106524<sup>T</sup>. This study provides more comprehensive insight into As-resistant bacteria and could be applied to the bioremediation of As and other heavy metals in the future.
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    Item type:Publication,
    Pisciglobus halotolerans gen. nov., sp. nov., isolated from fish sauce
    (2011-07-01)
    Tanasupawat, Somboon
    ;
    Thongsanit, Jaruwan
    ;
    Thawai, Chitti
    ;
    Lee, Keun Chul
    ;
    Lee, Jung Sook
    Two strains of Gram-stain-positive, catalase-negative, tetrad-forming cocci, C01 <sup>T</sup> and C02, were isolated in Thailand from fish sauce. They were facultatively anaerobic, non-motile and non-sporeforming bacteria. These strains produced L-lactic acid from glucose. They grew at pH 5.0-9.0, at 15-40 deg;C and in the presence of 10% (w/v) NaCl. The dominant fatty acid was C18: 1ω9c. The DNA G+C contents of strains C01 <sup>T</sup> and C02 were 38.6 and 38.7 mol%, respectively. Strain C01T was related most closely to Desemzia incerta DSM 20581 <sup>T</sup>, with a 16S rRNA gene sequence similarity of 96.9 %. The strains could be distinguished clearly from D. incerta DSM 20581 <sup>T</sup> based on cell morphology, physiological and biochemical characteristics and low levels of DNA-DNA relatedness. On the basis of the data presented, strains C01 <sup>T</sup> and C02 are considered to represent a novel species of a new genus in the Bacillus-Lactobacillus cluster, for which the name Pisciglobus halotolerans gen. nov., sp. nov. is proposed. The type strain of Pisciglobus halotolerans is C01T (=KCTC 13150 <sup>T</sup> =TISTR 1958 <sup>T</sup> =PCU 316T). © 2011 IUMS.
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