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Author: search.filters.author.Leksawasdi, N.Has files: No

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    Pretreated and enzyme-hydrolyzed peanut shell for cultivation of clostridium sp. g10
    (2021-01-01)
    Sanguanchaipaiwong, V.
    ;
    Yoddamnern, P.
    ;
    Matngammnuang, W.
    ;
    Leksawasdi, N.
    Peanut shell (PS) is an agricultural residue and generated in large quantities at the peanut processing location. Normally, these lignocellulosic materials are utilized as feed or disposed of to landfill or burnt. In this research, PS was pretreated, enzymatically hydrolyzed, and utilized as a carbon source for Clostridium sp. G10 to produce butanol through acetone-butanol-ethanol (ABE) fermentation. PS was pretreated with H<inf>2</inf>SO<inf>4</inf> and NaOH (0- 1.0M). The highest reducing sugar concentration in the supernatant obtained from 0.4M H2SO4 was 23.1 g/L. Subsequently, the solid portion of pretreated PS was hydrolyzed at 50 °C for 24 h using commercial cellulose (ACCELLERASE™ 1500) with various treatment times duration and enzyme activity to solid ratios. The result suggested that enzyme activityof pretreated PS weight ratio of 1250 carboxymethyl cellulose (CMC) U:1 g yielded the maximum concentration of reducing sugars(12.74 g/L). Mixture of acidic pretreatment hydrolysate and enzyme hydrolysate contained41.2 g/L glucose concentration. Clostridium sp. G10 was then cultured in three conditions; T6 medium with 45 g/L glucose as a control, T6 medium prepared with 45 g/L reducing sugar equivalent of PS hydrolysate, and PS hydrolysate with 45 g/L reducing sugar equivalent at 37°C for 168 h. The implementation ofPS hydrolysate solely as a carbon source produced the butanol concentration of 10.8 g/L. This was not significantly different (p < 0.05) from using T6 medium with glucose (9.3 g/L). The highest butanol production of 15.2 g/Lwas yielded from T6 medium with PS hydrolysate. It could be concluded that hydrolyzed PS could be utilized as a carbon source for production of butanol and ethanol.
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    Enzymatic hydrolysis of cassava stems for butanol production of isolated Clostridium sp.
    (2020-02-01)
    Saekhow, B.
    ;
    Chookamlang, S.
    ;
    Na-u-dom, A.
    ;
    Leksawasdi, N.
    ;
    Sanguanchaipaiwong, V.
    This research focused on the hydrolysis of cassava stems (CS) and subsequent utilization as a carbon source for the cultivation of isolated Clostridium sp. To yield the highest amount of reducing sugars (RS), the studies on the pretreatment with sodium hydroxide (NaOH) and the hydrolysis with cellulases, amylases, and mixed enzymes were carried out. Afterwards, the hydrolysate was utilized for the cultivation of isolated Clostridium sp. Experimental results revealed that CS after 1.0 M NaOH pretreatment at 121 °C for 15 min and cellulase hydrolysis (Accellerase® 1500, 2500 CMC U/g CS) obtained 10.94 ± 0.29 g/L RS concentration. Hydrolysis of CS with amylases (Termamyl® 120, 1.2 U/g CS and AMG 300L™ 3.5 U/g CS) provided 34.85 ± 0.75 g/L RS and the maximum RS amount of 47.90 ± 0.39 g/L was obtained from the hydrolysis with mixed enzymes (Termamyl® 120, 1.2 U/g CS, AMG 300L™ 3.5 U/g CS followed by Accellerase® 1500, 2500 CMC U/g CS). From the cultivation of Clostridium sp. G10 using CS hydrolysate, the highest dry cell weight concentration of 1.28 ± 0.07 g/L was obtained with 11.68 ± 0.31 g/L butanol. It could be concluded that CS hydrolysate was comparable with glucose for utilization as a carbon source for butanol production.