KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/123456789/1

Browse

Settings

Author: search.filters.author.Lerdpiriyaskulkij, NateeSubject: search.filters.subject.Alizarin complexone

Search Results

Now showing 1 - 1 of 1
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Ultrasonic-driven chemical reduction synthesis of alizarin complexone-modified gold nanoparticles for dual-signal colorimetric and fluorometric sensing of histamine in seafood products
    (2024-12-01)
    Phoungsiri, Ampika
    ;
    Lerdpiriyaskulkij, Natee
    ;
    Mathaweesansurn, Arjnarong
    ;
    Detsri, Ekarat
    Alizarin complexone-modified gold nanoparticles (Au<sup>0</sup>-NPs<inf>ALz</inf>) were synthesized using a proposed ultrasonic irradiation-assisted chemical reduction method. Ultrasonic irradiation powers, reaction time and alizarin complexone concentration had been proven to be the main parameters for controlling the nucleation and growth of Au<sup>0</sup>-NPs<inf>ALz</inf>. In the synthesized ultrasonic irradiation-assisted chemical reduction conditions, Au<sup>0</sup>-NPs<inf>ALz</inf> had a spherical oriented morphology with a uniform size of 17.84 ± 1.37 nm and are shiny red with a surface plasmon resonance (SPR) of 535 nm. A rapid colorimetric and fluorometric dual-mode detection strategy for selective detection of histamine in seafood was developed based on the self-assembly of Au<sup>0</sup>-NPs<inf>ALz</inf>-Ni (II) complexes. Ni (II) can capture the histamine molecules close to Au<sup>0</sup>-NPs<inf>ALz</inf> surfaces, making changes in the colorimetric and fluorometric responses of the solution. The quantitative analysis of histamine was realized through the variation of dual-signal colorimetric and fluorometric responses. Such Au<sup>0</sup>-NPs<inf>ALz</inf> sensor offered good detection sensitivity for histamine with a detection limit (LOD) of 59.32 μmol L<sup>−1</sup> and 116.20 μmol L<sup>−1</sup> and wide linear response within the range of 10–10000 μmol L<sup>−1</sup> (R<sup>2</sup> = 0.9952) and 100–5000 μmol L<sup>−1</sup> (R<sup>2</sup> = 0.9947) for colorimetric and fluorometric measurement, respectively. Recoveries ranging from 94.99 to 103.29 % and 97.67–106.88 % for colorimetric and fluorometric assay were obtained, showing low levels of matrix effects. Particularly, the results of the dual-mode sensor were also validated by comparing with the HPLC method for improving the assay accuracy and dependability. Ultimately, the developed Au<sup>0</sup>-NPs<inf>ALz</inf> colorimetric and fluorometric probe performs excellently in practical applications, with promising results for detecting histamine in seafood products.