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Author: search.filters.author.Lerdpiriyaskulkij, NateeSubject: search.filters.subject.Gold nanoparticles

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    Smartphone-based disposable cotton-swab sensor loaded with creatinine/Cu–chlorophyllin–stabilized AuNPs for ultrasensitive RGB colorimetric detection of mercury (II) ions in aquatic environments
    (2026-05-01)
    Lerdpiriyaskulkij, Natee
    ;
    Detsri, Ekarat
    ;
    Teerasong, Saowapak
    ;
    Chansai, Sarayute
    ;
    Mathaweesansurn, Arjnarong
    A simple, rapid, selective, and sensitive colorimetric sensor based on a disposable cotton swab loaded with Au nanoparticle–modified Cu-chlorophyllin (Au<sup>0</sup>–NPs<inf>CHL</inf>) and creatinine was developed for mercury (II) detection in aquatic environments using smartphone–based RGB analysis. Au<sup>0</sup>–NPs<inf>CHL</inf> were synthesized by ultrasonic-assisted chemical reduction, employing Cu-chlorophyllin as a stabilizing and NaBH<inf>4</inf> as a reducing agent. A vivid red Au<sup>0</sup>–NPs<inf>CHL</inf> colloidal (7.96 ± 0.29 nm) with a sharp SPR peak at 515 nm was successfully obtained. An aliquot of 75 µL of Au<sup>0</sup>-NPs<inf>CHL</inf> (0.103 ± 0.03 nmol L<sup>−1</sup>) and 25 µL of creatinine solution (40 mg L<sup>−1</sup>) were sequentially loaded into an 8.0 × 0.5 cm cotton swab, separated by a 0.5 cm air gap. Detection began by immersing the swab into Hg<sup>2+</sup>–contaminated samples for 3 min, allowing Hg<sup>2+</sup> adsorption. Breaking the swab generated pressure differential, which, along with gravity, drove the Au<sup>0</sup>–NPs<inf>CHL</inf> to mix with creatinine. The resulting mixture migrated toward the swab tip by capillary action and reacted with adsorbed Hg<sup>2+</sup> through metallophilic 5d<sup>10</sup>–5d<sup>10</sup> interactions. Creatinine acted as a bridging ligand, inducing Au<sup>0</sup>–NPs<inf>CHL</inf> aggregation and shifting the SPR to 620 nm, causing a visible red-to-blue transition. This sensor enables rapid and visual detection and quantitative evaluation via smartphone RGB analysis. The system demonstrated excellent linearity from 1 to 100 µg L<sup>−1</sup> with a low LOD of 0.82 µg L<sup>−1</sup>. Recoveries of 97.6–101.8 % confirmed high accuracy and minimal matrix interference. The disposable cotton swab RGB sensor provides a rapid, portable, and practical tool for on-site primary assessment of Hg<sup>2+</sup> contamination in environmental samples.
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    Ultrasonic-driven chemical reduction synthesis of alizarin complexone-modified gold nanoparticles for dual-signal colorimetric and fluorometric sensing of histamine in seafood products
    (2024-12-01)
    Phoungsiri, Ampika
    ;
    Lerdpiriyaskulkij, Natee
    ;
    Mathaweesansurn, Arjnarong
    ;
    Detsri, Ekarat
    Alizarin complexone-modified gold nanoparticles (Au<sup>0</sup>-NPs<inf>ALz</inf>) were synthesized using a proposed ultrasonic irradiation-assisted chemical reduction method. Ultrasonic irradiation powers, reaction time and alizarin complexone concentration had been proven to be the main parameters for controlling the nucleation and growth of Au<sup>0</sup>-NPs<inf>ALz</inf>. In the synthesized ultrasonic irradiation-assisted chemical reduction conditions, Au<sup>0</sup>-NPs<inf>ALz</inf> had a spherical oriented morphology with a uniform size of 17.84 ± 1.37 nm and are shiny red with a surface plasmon resonance (SPR) of 535 nm. A rapid colorimetric and fluorometric dual-mode detection strategy for selective detection of histamine in seafood was developed based on the self-assembly of Au<sup>0</sup>-NPs<inf>ALz</inf>-Ni (II) complexes. Ni (II) can capture the histamine molecules close to Au<sup>0</sup>-NPs<inf>ALz</inf> surfaces, making changes in the colorimetric and fluorometric responses of the solution. The quantitative analysis of histamine was realized through the variation of dual-signal colorimetric and fluorometric responses. Such Au<sup>0</sup>-NPs<inf>ALz</inf> sensor offered good detection sensitivity for histamine with a detection limit (LOD) of 59.32 μmol L<sup>−1</sup> and 116.20 μmol L<sup>−1</sup> and wide linear response within the range of 10–10000 μmol L<sup>−1</sup> (R<sup>2</sup> = 0.9952) and 100–5000 μmol L<sup>−1</sup> (R<sup>2</sup> = 0.9947) for colorimetric and fluorometric measurement, respectively. Recoveries ranging from 94.99 to 103.29 % and 97.67–106.88 % for colorimetric and fluorometric assay were obtained, showing low levels of matrix effects. Particularly, the results of the dual-mode sensor were also validated by comparing with the HPLC method for improving the assay accuracy and dependability. Ultimately, the developed Au<sup>0</sup>-NPs<inf>ALz</inf> colorimetric and fluorometric probe performs excellently in practical applications, with promising results for detecting histamine in seafood products.