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Item type:Publication, Improved biohydrogen production by immobilized cells of the green alga Tetraspora sp. CU2551 incubated under aerobic condition(2020-10-01) ;Maswanna, Thanaporn ;Lindblad, PeterManeeruttanarungroj, CherdsakThe green alga Tetraspora sp. CU2551 was previously investigated and showed enhanced H<inf>2</inf> production under anaerobic (Ar purged) conditions by cells immobilized in a calcium alginate matrix (Maswanna et al., Biomass Bioenergy 111:88–95, 2018). Here, we report successful H<inf>2</inf> production in entrapped cells under aerobic conditions. The most favorable immobilization condition observed was 4% (w/v) final alginate concentration after gelation, 2.80- to 3.35-mm beads, and a biomass content of 0.125 mg DW mL<sup>−1</sup> alginate. H<inf>2</inf> production increased when the immobilized cells were incubated in S-deprived media which could be repeated up to six times when using refreshed media. After six cycles, the H<inf>2</inf> production reached 12.8 ± 0.9 mL H<inf>2</inf> 25 mL<sup>−1</sup> of medium, corresponding to a rate of 182 ± 20 nmol mg<sup>−1</sup> DW h<sup>−1</sup>, which was significantly higher than previously observed for other microalgae. Thus, our results demonstrate a potential for photobiological H<inf>2</inf> production using immobilized Tetraspora sp. CU2551 cells, grown under ambient aerobic conditions.
