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Use of Ag-Au-ICG to increase fluorescence image of human hepatocellular carcinoma cell lines
(2023-01-01)
Sittisart, Pattarapol
;
Locharoenrat, Kitsakorn
Indocyanine green (ICG) is effective for a variety of applications including liver tumour imaging and operates in the near-infrared window. Agents for near-infrared imaging are, however, still in clinical development. The present study aimed to prepare and investigate fluorescence emission properties of ICG in combination with Ag-Au in order to enhance their specific interactions with human hepatocellular carcinoma cell lines (HepG-2). The Ag-Au-ICG complex was prepared via physical adsorption, and hence evaluated for fluorescence spectra using a spectrophotometer. Ag-Au-ICG at an optimised dosage (Ag-Au:ICG = 0.0147:1 molar ratio) in Intralipid medium was added to HepG-2 to observe the maximum fluorescence signal intensity, which further enhanced HepG-2 contrast fluorescence. Ag-Au-ICG served as a fluorescence enhancer bound onto the liposome membrane, whilst free Ag, Au, and pure ICG induced low levels of cytotoxicity in HepG-2 and a normal human cell line. Thus, our findings provided new insights for the liver cancer imaging.Highlights Concentration-dependent fluorescence peaking in the near-infrared window revealed ICG aggregation in Ag-Au molecules. Ag-Au-ICG fluorescence intensity depended strongly on the environmental media. Human hepatocellular carcinoma cell lines treated with Ag-Au-ICG in Intralipid enhanced the contrast of fluorescence microscopy images by decreasing the level of scattering in the cell lines with the contrast values being approximately five times those observed in pure ICG in Intralipid.
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Effects of gold nanoparticles on fluorescence polarization and emission spectra of rhodamine 6G solution
(2018-01-01)
Damrongsak, Pattareeya
;
Rammarat, Ekkachai
;
Locharoenrat, Kitsakorn
Fluorescence polarization and emission spectra of Rhodamine 6G solution with gold nanoparticles have been studied. It is found that the emission intensities tend to be low when the gold nanoparticles are added into the solution partly due to the energy transfer from the Rhodamine 6G to gold nanoparticles. The fluorescence polarization increases as the concentration ratio of the gold and Rhodamine 6G is more than 124 and decreases as the ratio is about 1115. The enhancement and reduction of fluorescence polarization values are understood as the shortening of the fluorescence lifetime and a temperature effect from the heating of gold nanoparticles, respectively.
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Studies of concentration dependence of the fluorescent quantum yield from rhodamine 6G and Au–Pd core–shell nanorods, using a response surface methodology
(2017-01-01)
Rammarat, Ekkachai
;
Locharoenrat, Kitsakorn
;
Yindeesuk, Witoon
;
Damrongsak, Pattareeya
We have applied a response surface approach to study the fluorescence quantum yield (FQY) of rhodamine 6G (Rh6G) mixed with Au–Pd core–shell nanorods (Au–Pd NRs). The FQYs have been measured for the Rh6G concentrations varying from 3.53×10–7 to 1.70×10–6 mol/L and the concentrations of Au–Pd NRs from 7.06×10–6 to 1.36×10–4 mol/L. Our experimental results testify that the FQY depends notably on the proportions of Rh6G and Au–Pd NRs. A specific relationship between the FQY and the concentrations has also been confirmed by a response surface plot. It is found that the discrepancy between the experiment and the calculations is less than 2%.

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