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Physiological and Environmental Factors Influencing Hydrogen Production by Unicellular Green Alga Monoraphidium sp. KMITL-1
(2025-10-01)
Krutpan, Varanya
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Supakriangkrai, Thaninthorn
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Lohasupthawee, Pana
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Phunpruch, Saranya
With the growing global energy demand and the urgent need to reduce carbon emissions, hydrogen (H<inf>2</inf>) has emerged as a promising clean energy carrier. Among various biological H<inf>2</inf> production, green algae present a sustainable and eco-friendly alternative due to their ability to produce H<inf>2</inf> via photobiological pathways. This study aimed to investigate H<inf>2</inf> production by unicellular green alga Monoraphidium sp. KMITL-1, isolated from hydroponic water at the Plant Tissue Culture Laboratory, King Mongkut’s Institute of Technology Ladkrabang. The taxonomic identity of the strain, belonging to the genus Monoraphidium within the Selenastraceae family, was confirmed through morphological observation and molecular characterization using 23S plastid rRNA gene sequencing. Various physiological and environmental parameters influencing H<inf>2</inf> production were evaluated, including cell age, cell density, nutrient deprivation, carbon source, pH, temperature, and light intensity. A 24-hour-old culture with an OD<inf>750</inf> of 0.8 exhibited a significant increase in H<inf>2</inf> production. The optimal medium was potassium-deprived Tris-acetate-phosphate (TAP-K) supplemented with glucose at a concentration of 350 mmol C-atom L-1. The ideal environmental conditions for H<inf>₂</inf> production were pH 7.2, a temperature of 30 °C, and a light intensity of 60 μmol photons m-2 s-1. Under these optimized conditions, Monoraphidium sp. KMITL-1 achieved a maximum H<inf>2</inf> production rate of 67.976 ± 1.096 μmol H<inf>2</inf> mg Chl-1 h-1 and a cumulative H<inf>2</inf> yield of 3,190.436 ± 2.219 μmol H<inf>2</inf> mg Chl-1 after 72 h of incubation. These results highlight the potential of Monoraphidium sp. KMITL-1 for large-scale biohydrogen production and its applicability in the development of sustainable energy technologies.
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Development of a Broad-Spectrum Pan-Mpox Vaccine via Immunoinformatic Approaches
(2025-08-01)
Puagsopa, Japigorn
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Jumpalee, Panuwid
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Dechanun, Sittichoke
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Choengchalad, Sukanya
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Lohasupthawee, Pana
Monkeypox virus (MPXV) has caused 148,892 confirmed cases and 341 deaths from 137 countries worldwide, as reported by the World Health Organization (WHO), highlighting the urgent need for effective vaccines to prevent the spread of MPXV. Traditional vaccine development is low-throughput, expensive, time consuming, and susceptible to reversion to virulence. Alternatively, a reverse vaccinology approach offers a rapid, efficient, and safer alternative for MPXV vaccine design. Here, MPXV proteins associated with viral infection were analyzed for immunogenic epitopes to design multi-epitope vaccines based on B-cell, CD4+, and CD8+ epitopes. Epitopes were selected based on allergenicity, antigenicity, and toxicity parameters. The prioritized epitopes were then combined via peptide linkers and N-terminally fused to various protein adjuvants, including PADRE, beta-defensin 3, 50S ribosomal protein L7/12, RS-09, and the cholera toxin B subunit (CTB). All vaccine constructs were computationally validated for physicochemical properties, antigenicity, allergenicity, safety, solubility, and structural stability. The three-dimensional structure of the selected construct was also predicted. Moreover, molecular docking and molecular dynamics (MD) simulations between the vaccine and the TLR-4 immune receptor demonstrated a strong and stable interaction. The vaccine construct was codon-optimized for high expression in the E. coli and was finally cloned in silico into the pET21a (+) vector. Collectively, these results could represent innovative tools for vaccine formulation against MPXV and be transformative for other infectious diseases.
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Mulberroside A Accumulation in Mulberry Plantlets from In Vitro Culture and Hydroponic System
(2023-12-29)
Damrongmongcolkul, Natvara
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Phunpruch, Saranya
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Lohasupthawee, Pana
In this study, nodal explants cultured in MS medium supplemented with BAP 4mg/l and NAA 0.5 mg/l induced 3.33 shoots per explant within 4 weeks. The elongated multiple shoots were transferred to rooting media. The best root development was found on MS medium without PGRs within 10 days. The acclimatized plants were transferred to a deep-water hydroponic system with different concentrations of MS nutrient solution without sucrose addition. The results showed that mulberry plants cultivated in 1/8 MS nutrient solution gave the highest growth index. For mulberroside A determination, the results showed that mulberroside A accumulation in roots were higher than in shoots of mulberry plants both for in vitro culture and in the hydroponic system. In the case of in vitro culture, mulberroside A accumulation in roots was 20.61 mg/g DW, and in shoots was 11.98 mg/g DW. In hydroponic system, mulberroside A accumulation in roots was 17.58 mg/g DW, and in shoots was 2.34 mg/g DW. We also found that the higher concentration of MS nutrient solution, the more mulberroside A accumulation in roots of mulberry plants cultivated in the hydroponic system.
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Mass Propagation System of Strawberry (Fragaria × ananassa) Microshoots by Liquid Shake Culture
(2022-01-01)
Chomboon, Prakarn
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Lohasupthawee, Pana
The future of strawberry propagation and sales is very positive, so an efficient mass propagation system for strawberry (Fragaria × ananassa) was developed in this study. Strawberry leaf explants successfully induced green compact calli in semi-solid MS medium supplemented with 1 mg/l 2,4-D and 0.5 mg/l BA within 2 weeks. Subculture the calli in semi-solid MS medium supplemented with 1 mg/l TDZ and 0.2 mg/l 2,4-D produced 3.8 microshoots per callus within 49-56 days. These leafderived calli with adventitious shoots were cultured with 0.1 mg/l GA<inf>3</inf> and shaken at 120 rpm. The number of shoots increased from 15.3 shoots to 43.6 shoots per culture (100 ml) within 2 weeks, but all were hyperhydric shoots. Hyperhydric shoots recovered to normal shoots within 1 month when cultured on semi-solid MS medium with 8 g/l agar. Shoots formed roots on semi-solid MS medium supplemented with 0.5 mg/l IBA within 2 weeks. Rooted plantlets were acclimatized for 1 week before being transferred to the field at Bendito farm, where the strawberry plants were grown until maturity.

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