KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/123456789/1

Browse

Settings

Author: search.filters.author.Maswanna, ThanapornSubject: search.filters.subject.Biohydrogen

Search Results

Now showing 1 - 2 of 2
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    A newly isolated green alga Chlorella sp. KLSc59: potential for biohydrogen production
    (2020-10-01)
    Sirawattanamongkol, Thanaporn
    ;
    Maswanna, Thanaporn
    ;
    Maneeruttanarungroj, Cherdsak
    Hydrogen production from microalgae has attracted considerable attention due to its high energy content and as a renewable and environmentally friendly energy source. Various strains of microalgae have been reported to produce “biohydrogen”, but screening for new strains is still necessary to discover strains with higher hydrogen yields. A newly isolated hydrogen-producing green alga was screened and labeled Chlorella sp. KLSc59. The effect of extracellular pH, light intensity, external carbon sources, reducing agents, and nutrient deprivation on biohydrogen production of Chlorella sp. KLSc59 were investigated. Hydrogen yield was higher under anaerobic conditions. Under external pH 7.2 with 53.2 μmol photons m<sup>−2</sup> s<sup>−1</sup> light intensity and using acetate as a carbon source, the optimum hydrogen yield was 281 μmol H<inf>2</inf> mg<sup>−1</sup> Chl. Nutrient deprivation reduced the hydrogen yield. Several reducing agents were assessed, and 1 mM ethanol enhanced yield by 3 times for 850 μmol H<inf>2</inf> mg<sup>−1</sup> Chl, and 1 mM sodium dithionite increased yield by 2.7 times for 750 μmol H<inf>2</inf> mg<sup>−1</sup> Chl. Significantly, our new strain showed higher hydrogen yields ranging from 1.5 to 68 times compared with other microalgae. Thus, Chlorella sp. KLSc59 showed valuable potential for biohydrogen production.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Improved biohydrogen production by immobilized cells of the green alga Tetraspora sp. CU2551 incubated under aerobic condition
    (2020-10-01)
    Maswanna, Thanaporn
    ;
    Lindblad, Peter
    ;
    Maneeruttanarungroj, Cherdsak
    The green alga Tetraspora sp. CU2551 was previously investigated and showed enhanced H<inf>2</inf> production under anaerobic (Ar purged) conditions by cells immobilized in a calcium alginate matrix (Maswanna et al., Biomass Bioenergy 111:88–95, 2018). Here, we report successful H<inf>2</inf> production in entrapped cells under aerobic conditions. The most favorable immobilization condition observed was 4% (w/v) final alginate concentration after gelation, 2.80- to 3.35-mm beads, and a biomass content of 0.125 mg DW mL<sup>−1</sup> alginate. H<inf>2</inf> production increased when the immobilized cells were incubated in S-deprived media which could be repeated up to six times when using refreshed media. After six cycles, the H<inf>2</inf> production reached 12.8 ± 0.9 mL H<inf>2</inf> 25 mL<sup>−1</sup> of medium, corresponding to a rate of 182 ± 20 nmol mg<sup>−1</sup> DW h<sup>−1</sup>, which was significantly higher than previously observed for other microalgae. Thus, our results demonstrate a potential for photobiological H<inf>2</inf> production using immobilized Tetraspora sp. CU2551 cells, grown under ambient aerobic conditions.